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Gene Expression Data
Assay Details
Assay
Reference: J:256987 Shi B, et al., MORC2B is essential for meiotic progression and fertility. PLoS Genet. 2018 Jan;14(1):e1007175
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7509245
Gene symbol: Sycp2
Gene name: synaptonemal complex protein 2
Antibody: anti-SYCP2 (1918)
Detection system: Secondary antibody coupled to Texas Red
Assay notes: Two Sycp2 antibodies were described, but the one used in this assay was not detailed. Anti-SYCP2 (GP21) MGI:5785015 may have been used in this assay.
Results
Specimen 4A SYCP2 +/+: postnatal (more )
Note: Double labeled: red - Sycp2; green - Sycp1 (assay MGI:7509244).
Structure Level Pattern Image Note
TS28: spermatocyte Present 4A +/+
Specimen 4A SYCP2 -/-: postnatal; Morc2btm1Jw/Morc2btm1Jw (more )
Note: Double labeled: red - Sycp2; green - Sycp1 (assay MGI:7509244).
Structure Level Pattern Image Note
TS28: spermatocyte Present 4A -/-
Specimen 4B SYCP2 +/+: embryonic day 17.5 (more )
Note: Double labeled: red - Sycp2; green - Sycp1 (assay MGI:7509244).
Structure Level Pattern Image Note
TS25: oocyte Present 4B +/+
Specimen 4B SYCP2 -/-: embryonic day 17.5; Morc2btm1Jw/Morc2btm1Jw (more )
Note: Double labeled: red - Sycp2; green - Sycp1 (assay MGI:7509244).
Structure Level Pattern Image Note
TS25: oocyte Present 4B -/-
Specimen 5A SYCP2 leptotene: postnatal day 25 (more )
Note: Double labeled: red - Sycp2; green - H2ax (assay MGI:7509248).
Structure Level Pattern Image Note
TS28: spermatocyte Present 5A leptotene merged , 5A leptotene SYCP2
Specimen 5A SYCP2 zygotene: postnatal day 25 (more )
Note: Double labeled: red - Sycp2; green - H2ax (assay MGI:7509248).
Structure Level Pattern Image Note
TS28: spermatocyte Present 5A zygotene merged , 5A zygotene SYCP2
Specimen 5A SYCP2 pachytene: postnatal day 25 (more )
Note: Double labeled: red - Sycp2; green - H2ax (assay MGI:7509248).
Structure Level Pattern Image Note
TS28: spermatocyte Present 5A pachytene SYCP2 , 5A pachytene merged
Specimen 5A SYCP2 diplotene: postnatal day 25 (more )
Note: Double labeled: red - Sycp2; green - H2ax (assay MGI:7509248).
Structure Level Pattern Image Note
TS28: spermatocyte Present 5A diplotene merged , 5A diplotene SYCP2
Specimen 5B SYCP2 leptotene: postnatal day 25; Morc2btm1Jw/Morc2btm1Jw (more )
Note: Double labeled: red - Sycp2; green - H2ax (assay MGI:7509248).
Structure Level Pattern Image Note
TS28: spermatocyte Present 5B leptotene merged , 5B leptotene SYCP2
Specimen 5B SYCP2 zygotene-like: postnatal day 25; Morc2btm1Jw/Morc2btm1Jw (more )
Note: Double labeled: red - Sycp2; green - H2ax (assay MGI:7509248).
Structure Level Pattern Image Note
TS28: spermatocyte Present 5B zygotene-like merged , 5B zygotene-like SYCP2
Specimen 5B SYCP2 pachytene-like: postnatal day 25; Morc2btm1Jw/Morc2btm1Jw (more )
Note: Double labeled: red - Sycp2; green - H2ax (assay MGI:7509248).
Structure Level Pattern Image Note
TS28: spermatocyte Present 5B pachytene-like SYCP2 , 5B pachytene-like merged
Specimen 6A SYCP2 +/-: postnatal day 18; Morc2btm1Jw/Morc2b+ (more )
Note: Double labeled: red - Sycp2; green - Rpa2 (assay MGI:7509249).
Structure Level Pattern Image Note
TS28: spermatocyte Present 6A +/- merged , 6A +/- SYCP2
Specimen 6A SYCP2 -/-: postnatal day 18; Morc2btm1Jw/Morc2btm1Jw (more )
Note: Double labeled: red - Sycp2; green - Rpa2 (assay MGI:7509249).
Structure Level Pattern Image Note
TS28: spermatocyte Present 6A -/- SYCP2 , 6A -/- merged
Specimen 6B SYCP2 +/-: postnatal day 18; Morc2btm1Jw/Morc2b+ (more )
Note: Double labeled: red - Sycp2; green - Meiob (assay MGI:7509251).
Structure Level Pattern Image Note
TS28: spermatocyte Present 6B +/- merged , 6B +/- SYCP2
Specimen 6B SYCP2 -/-: postnatal day 18; Morc2btm1Jw/Morc2btm1Jw (more )
Note: Double labeled: red - Sycp2; green - Meiob (assay MGI:7509251).
Structure Level Pattern Image Note
TS28: spermatocyte Present 6B -/- merged , 6B -/- SYCP2
Specimen 6C SYCP2 +/-: postnatal day 18; Morc2btm1Jw/Morc2b+ (more )
Note: Double labeled: red - Sycp2; green - Rad51 (assay MGI:7509252).
Structure Level Pattern Image Note
TS28: spermatocyte Present 6C +/- merged , 6C +/- SYCP2
Specimen 6C SYCP2 -/-: postnatal day 18; Morc2btm1Jw/Morc2btm1Jw (more )
Note: Double labeled: red - Sycp2; green - Rad51 (assay MGI:7509252).
Structure Level Pattern Image Note
TS28: spermatocyte Present 6C -/- merged , 6C -/- SYCP2
Specimen 6D SYCP2 +/-: postnatal day 18; Morc2btm1Jw/Morc2b+ (more )
Note: Double labeled: red - Sycp2; green - Dmc1 (assay MGI:7509253).
Structure Level Pattern Image Note
TS28: spermatocyte Present 6D +/- merged , 6D +/- SYCP2
Specimen 6D SYCP2 -/-: postnatal day 18; Morc2btm1Jw/Morc2btm1Jw (more )
Note: Double labeled: red - Sycp2; green - Dmc1 (assay MGI:7509253).
Structure Level Pattern Image Note
TS28: spermatocyte Present 6D -/- merged , 6D -/- SYCP2
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory