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Gene Expression Data
Assay Details
Assay
Reference: J:320624 Tan BSN, et al., Regulation of amino acid transporters in pluripotent cell populations in the embryo and in culture; novel roles for sodium-coupled neutral amino acid transporters. Mech Dev. 2016 Aug;141:32-39
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7506230
Gene symbol: Slc38a2
Gene name: solute carrier family 38, member 2
Antibody: anti-SNAT2 (C-12; sc-33444)
Detection system: Secondary antibody coupled to Alexa Fluor 568
Results
Specimen 1B 2-cell: embryonic day 1.5 (more )
Note: 2-cell embryo. Double labeled: red - Slc38a2; green - Pou5f1 (assay MGI:7506229).
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present 1B 2-cell SNAT2 , 1B 2-cell merge Expression is cytoplasmic and nuclear.
Specimen 1B 4-cell: embryonic day 2.0 (more )
Note: 4-cell embryo. Double labeled: red - Slc38a2; green - Pou5f1 (assay MGI:7506229).
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Present 1B 4-cell Merge , 1B 4-cell SNAT2 Expression is cytoplasmic and nuclear.
Specimen 1B Morula: embryonic day 2.25 (more )
Note: Morula stage embryo. Double labeled: red - Slc38a2; green - Pou5f1 (assay MGI:7506229).
Structure Level Pattern Image Note
TS3: compacted morula Present 1B Morula Merge , 1B Morula SNAT2 There is diffuse expression throughout the cytoplasm.
Specimen 2B E3.5i: embryonic day 3.5 (more )
Note: Early blastocyst stage embryo. Double labeled: red - Slc38a2; green - Pou5f1 (assay MGI:7506229).
Structure Level Pattern Image Note
TS4: trophectoderm Present 2B E3.5i Merge , 2B E3.5i SNAT2
TS4: inner cell mass Weak 2B E3.5i SNAT2 , 2B E3.5i Merge
Specimen 2B E3.5ii: embryonic day 3.5 (more )
Note: Fully expanded blastocyst stage embryo. Double labeled: red - Slc38a2; green - Pou5f1 (assay MGI:7506229).
Structure Level Pattern Image Note
TS4: inner cell mass Present 2B E3.5ii SNAT2 , 2B E3.5ii Merge
TS4: trophectoderm Present 2B E3.5ii SNAT2 , 2B E3.5ii Merge
Specimen 2B E4.5: embryonic day 4.5 (more )
Note: Blastocyst stage embryo. Double labeled: red - Slc38a2; green - Pou5f1 (assay MGI:7506229).
Structure Level Pattern Image Note
TS5: inner cell mass Present 2B E4.5 Merge , 2B E4.5 SNAT2
TS5: trophectoderm Present 2B E4.5 Merge , 2B E4.5 SNAT2
Specimen 3A: embryonic day 3.5 (more )
Note: Blastocyst stage embryo.
Structure Level Pattern Image Note
TS4: trophectoderm Present 3A Expression is nuclear.
Specimen 4A: embryonic day 5.5 (more )
Structure Level Pattern Image Note
TS8: embryo Present 4A SNAT2 , 4A SNAT2 detail , 4A SNAT2 right
TS8: primitive endoderm Present 4A SNAT2 , 4A SNAT2 detail , 4A SNAT2 right
TS8: extraembryonic component Weak 4A SNAT2 detail , 4A SNAT2 , 4A SNAT2 right
Specimen S1A 2-cell: embryonic day 1.5 (more )
Note: 2-cell embryo. Double labeled: red - Slc38a2; green - Pou5f1 (assay MGI:7506229).
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present S1B 2-cell SNAT2 , S1B 2-cell merge Expression is cytoplasmic and nuclear.
Specimen S1A 4-cell: embryonic day 2.0 (more )
Note: 4-cell embryo. Double labeled: red - Slc38a2; green - Pou5f1 (assay MGI:7506229).
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Present S1B 4-cell Merge , S1B 4-cell SNAT2 Expression is cytoplasmic and nuclear.
Specimen S1A Morula: embryonic day 2.25 (more )
Note: Morula stage embryo. Double labeled: red - Slc38a2; green - Pou5f1 (assay MGI:7506229).
Structure Level Pattern Image Note
TS3: compacted morula Present S1B Morula Merge , S1B Morula SNAT2 There is diffuse expression throughout the cytoplasm.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
MGI 6.23 		The Jackson Laboratory