Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:160043
Ng EL, et al., Rab22B is expressed in the CNS astroglia lineage and plays a role in epidermal growth factor receptor trafficking in A431 cells. J Cell Physiol. 2009 Dec;221(3):716-28
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:7495936
|
|
Gene symbol:
|
Rab31
|
|
Gene name:
|
RAB31, member RAS oncogene family
|
|
Antibody:
|
anti-Rab22B
|
|
Detection system:
|
Secondary antibody coupled to fluorescein
|
|
|
Results
|
Specimen
1B Rab22B (upper):
embryonic day 14.0
(more  )
Note: Double labeled: green - Rab31 (Rab22B); red - Nes (assay MGI:7495938).
Specimen
1B Rab22B (upper):
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 14.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Rab31 (Rab22B); red - Nes (assay MGI:7495938).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: brain |
radial glial cell |
Present |
|
1B Rab22B (upper)
,
1B Merge (upper)
|
Expression in nestin-expressing fibers.
|
Specimen
1B Rab22B (middle):
embryonic day 14.0
(more )
Note: Double labeled: green - Rab31 (Rab22B); red - Nes (assay MGI:7495939).
Specimen
1B Rab22B (middle):
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 14.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Rab31 (Rab22B); red - Nes (assay MGI:7495939).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: brain |
radial glial cell |
Present |
|
1B Rab22B (middle)
,
1B Merge (middle)
|
Expression in RC2-positive fibers.
|
Specimen
1B Rab22B (lower):
embryonic day 14.0
(more )
Note: Double labeled: green - Rab31 (Rab22B); red - A2B5.
Specimen
1B Rab22B (lower):
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 14.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Rab31 (Rab22B); red - A2B5.
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: brain |
radial glial cell |
Present |
Regionally restricted |
1B Merge (lower)
,
1B Rab22B (lower)
|
Prominent expression in cells negative for A2B5.
|
| TS22: brain |
oligodendrocyte |
Absent |
|
1B Merge (lower)
,
1B Rab22B (lower)
|
Expression largely absent in A2B5-positive cell bodies.
|
Specimen
E16 not shown:
embryonic day 16.0
(more )
Specimen
E16 not shown:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 16.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS24: brain |
radial glial cell |
Present |
|
|
|
| TS24: brain |
neural progenitor cell |
Absent |
|
|
No expression in neuron precursors.
|
Specimen
S1 Rab22B (upper):
embryonic day 18.0
(more )
Note: Double labeled: green - Rab31 (Rab22B); red - Nes (assay MGI:7495938).
Specimen
S1 Rab22B (upper):
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 18.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Rab31 (Rab22B); red - Nes (assay MGI:7495938).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: brain |
radial glial cell |
Weak |
|
S1_Merge (upper)
,
S1_Rab22B (upper)
|
Expression diminished at late embryonic stage. Expression in nestin-expressing cells
|
Specimen
S1 Rab22B (lower):
postnatal newborn
(more )
Note: Double labeled: green - Rab31 (Rab22B); red - Gfap (assay MGI:7495940).
Specimen
S1 Rab22B (lower):
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
postnatal newborn
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Rab31 (Rab22B); red - Gfap (assay MGI:7495940).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: brain |
radial glial cell |
Weak |
|
S1_Rab22B (lower)
,
S1_Merge (lower)
|
Expression diminished at newborn stage.
|
| TS27: brain |
astrocyte |
Absent |
|
S1_Merge (lower)
,
S1_Rab22B (lower)
|
No expression in Gfap-expressing cells.
|
Specimen
2 Rab22B Midbrain:
postnatal adult
(more )
Note: Double labeled: green - Rab31 (Rab22B); red - Gfap (assay MGI:7495940).
Specimen
2 Rab22B Midbrain:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Rab31 (Rab22B); red - Gfap (assay MGI:7495940).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: midbrain |
astrocyte |
Present |
|
2_Midbrain Merge
,
2_Midbrain Rab22B
|
Expression almost exclusively in Gfap-expressing astrocytes.
|
Specimen
2 Rab22B Cortex:
postnatal adult
(more )
Note: Double labeled: green - Rab31 (Rab22B); red - Gfap (assay MGI:7495940).
Specimen
2 Rab22B Cortex:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Rab31 (Rab22B); red - Gfap (assay MGI:7495940).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: cerebral cortex |
astrocyte of the cerebral cortex |
Present |
|
2_Cortex Merge
,
2_Cortex Rab22B
|
Expression almost exclusively in Gfap-expressing astrocytes.
|
Specimen
2 Rab22B Hippocampus:
postnatal adult
(more )
Note: Double labeled: green - Rab31 (Rab22B); red - Gfap (assay MGI:7495940).
Specimen
2 Rab22B Hippocampus:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Rab31 (Rab22B); red - Gfap (assay MGI:7495940).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: hippocampus |
hippocampal astrocyte |
Present |
|
2_Hippocampus Merge
,
2_Hippocampus Rab22B
|
Expression almost exclusively in Gfap-expressing astrocytes.
|
Specimen
2 Rab22B Cerebellum:
postnatal adult
(more )
Note: Double labeled: green - Rab31 (Rab22B); red - Gfap (assay MGI:7495940).
Specimen
2 Rab22B Cerebellum:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Rab31 (Rab22B); red - Gfap (assay MGI:7495940).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: cerebellum |
astrocyte of the cerebellum |
Present |
|
2_Cerebellum Rab22b
,
2_Cerebellum Merge
|
Expression almost exclusively in Gfap-expressing astrocytes.
|
Specimen
2 Rab22B Lateral ventricle:
postnatal adult
(more )
Note: Double labeled: green - Rab31 (Rab22B); red - Gfap (assay MGI:7495940).
Specimen
2 Rab22B Lateral ventricle:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Rab31 (Rab22B); red - Gfap (assay MGI:7495940).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: lateral ventricle |
astrocyte |
Present |
|
2_Lateral ventricle Rab22B
,
2_Lateral ventricle Merge
|
Expression almost exclusively in Gfap-expressing astrocytes.
|
Specimen
2 Rab22B Corpus callosum:
postnatal adult
(more )
Note: Double labeled: green - Rab31 (Rab22B); red - Gfap (assay MGI:7495940).
Specimen
2 Rab22B Corpus callosum:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Rab31 (Rab22B); red - Gfap (assay MGI:7495940).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: corpus callosum |
astrocyte |
Present |
|
2_Corpus callosum Rab22B
,
2_Corpus callosum Merge
|
Expression almost exclusively in Gfap-expressing astrocytes.
|
Specimen
3 Rab22B (upper):
postnatal adult
(more )
Note: Double labeled: green - Rab31 (Rab22B); red - Cnp (assay MGI:7495941).
Specimen
3 Rab22B (upper):
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Rab31 (Rab22B); red - Cnp (assay MGI:7495941).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: brain |
oligodendrocyte |
Absent |
|
3_Merge (upper)
,
3_Rab22B (upper)
|
No expression in CNP-expressing mature oligodendrocytes.
|
Specimen
3 Rab22B (middle):
postnatal adult
(more )
Note: Double labeled: green - Rab31 (Rab22B); red - Tubb3 (assay MGI:7495942).
Specimen
3 Rab22B (middle):
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Rab31 (Rab22B); red - Tubb3 (assay MGI:7495942).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: brain |
neuron |
Absent |
|
3_Merge (middle)
,
3_Rab22B (middle)
|
No expression in Tubb3-positive neuronal cell bodies.
|
Specimen
3 Rab22B (lower):
postnatal adult
(more )
Note: Double labeled: green - Rab31 (Rab22B); red - Gfap (assay MGI:7495940).
Specimen
3 Rab22B (lower):
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Rab31 (Rab22B); red - Gfap (assay MGI:7495940).
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: brain |
astrocyte |
Present |
Regionally restricted |
3_Merge (lower)
,
3_Rab22B (lower)
|
Expression confined to cell bodies, not in astrocytic processes.
|
|
Analysis Tools