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Gene Expression Data
Assay Details
Assay
Reference: J:324832 Sun Y, et al., Single-cell transcriptomic landscapes of the otic neuronal lineage at multiple early embryonic ages. Cell Rep. 2022 Mar 22;38(12):110542
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7493605
Gene symbol: Myt1
Gene name: myelin transcription factor 1
Antibody: anti-Myt1
Detection system: Secondary antibody
Results
Specimen 3E/F: embryonic day 11.5; Foxg1tm1.1(cre)Ddmo/Foxg1+, Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Multi-labeled: white - Myt1; green - Insm1 (assay MGI:7493531); red - Cre reporter; blue - Hoechst.
Structure Cell Type Level Pattern Image Note
TS20: vestibulo-cochlear ganglion Present 3E''' , 3E'' , 3F''' , 3F''
TS20: hindbrain neuron Present 3E''' , 3E''
Specimen 4A/B: embryonic day 9.5 (more )
Note: Multi-labeled: red - Myt1; green - Insm1 (assay MGI:7493531); white - Neurod1 (assay MGI:7493524); blue - Hoechst.
Structure Cell Type Level Pattern Image Note
TS16: cranial ganglion Absent 4B' , 4B''' , 4A''' , 4A' There is no expression in cells committed to be cochleo-vestibular ganglion cells.
TS16: future hindbrain neuron Present 4A''' , 4A'
Specimen 4C: embryonic day 10.5 (more )
Note: Triple labeled: green - Myt1; red - Neurod1 (assay MGI:7493524); blue - Hoechst.
Structure Level Pattern Image Note
TS18: cranial ganglion Present Regionally restricted 4C'' , 4C Expression is seen in cells committed to be cochleo-vestibular ganglion cells.
Specimen 4G: embryonic day 15.5 (more )
Note: Double labeled: red - Myt1; blue - Hoechst.
Structure Level Pattern Image Note
TS23: cochlear ganglion Present 4G
Specimen 4H: embryonic day 15.5 (more )
Note: Multi-labeled: red - Myt1; white - Sox2 (assay MGI:7493756); green - Tubb3 (assay MGI:7493858); blue - Hoechst.
Structure Cell Type Level Pattern Image Note
TS23: cochlear ganglion spiral ganglion neuron Present 4H''' , 4H
TS23: cochlear ganglion glial cell Absent 4H''' , 4H
Specimen 4I: postnatal day 1 (more )
Note: Double labeled: green - Myt1; blue - Hoechst.
Structure Level Pattern Image Note
TS27: cochlear ganglion Present 4I
TS27: vestibular ganglion Present 4I
Specimen 4I inset: postnatal day 1 (more )
Note: Triple labeled: green - Myt1; red - Pvalb (assay MGI:7493859); blue - Hoechst.
Structure Cell Type Level Pattern Image Note
TS27: cochlea epithelium cochlear inner hair cell Present 4I inset
TS27: cochlea epithelium cochlear outer hair cell Present 4I inset
Specimen 4K: postnatal day 30 (more )
Note: Multi-labeled: red - Myt1; green - Gata3 (assay MGI:7493860); white - Tubb3 (assay MGI:7493858); blue - Hoechst.
Structure Cell Type Level Pattern Image Note
TS28: cochlear ganglion type 1 spiral ganglion neuron Present 4K , 4K'''
TS28: cochlear ganglion type 2 spiral ganglion neuron Present 4K , 4K'''
Specimen Not shown: postnatal adult (more )
Structure Cell Type Level Pattern Image Note
TS28: vestibular ganglion neuron Present
Specimen S3D: embryonic day 15.5 (more )
Note: Double labeled: red - Myt1; blue - Hoechst.
Structure Cell Type Level Pattern Image Note
TS23: cochlear ganglion spiral ganglion neuron Present S3D'' , S3D'
Specimen S3E: embryonic day 15.5; Myt1tm1(EGFP/icre)Ldh/Myt1tm1(EGFP/icre)Ldh (more )
Note: Triple labeled: red - Myt1 protein; green - Myt1 reporter (assay MGI:7493750); blue - Hoechst.
Structure Level Pattern Image Note
TS23: cochlear ganglion Absent S3E' , S3E'' , S3F' , S3F''
Specimen 5E: embryonic day 13.5; Foxg1tm1.1(cre)Ddmo/Foxg1+, Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Multi-labeled: green - Myt1; white - Insm1 (assay MGI:7493531); red - Cre reporter; blue - Hoechst.
Structure Cell Type Level Pattern Image Note
TS22: vestibular ganglion neuron Present Regionally restricted 5F' , 5E''' , 5E' , 5F''' Expression is seen in superior, not inferior, vestibular ganglion cells.
Specimen S6D: embryonic day 13.5; Foxg1tm1.1(cre)Ddmo/Foxg1+, Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Multi-labeled: green - Myt1; white - Insm1 (assay MGI:7493531); red - Cre reporter; blue - Hoechst.
Structure Cell Type Level Pattern Image Note
TS22: cochlear ganglion neuron Present S6D'' , S6D'''
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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