Probe:	miR-194 probe
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	The probe used for this assay detects the expression of both family members.

Structure	Level	Pattern	Image	Note
TS27: cochlea epithelium	Present		2G Whole cochlea	Relatively weak expression in sensory epithelia in cochlea.
TS27: vestibulo-cochlear ganglion	Present		2G Whole cochlea	Robust expression in statoacoustic ganglion, including spiral ganglion.

Structure	Level	Pattern	Image	Note
TS27: cochlea epithelium	Present		2G Basilar membrane	Relatively weak expression in sensory epithelia in cochlea.

Structure	Level	Pattern	Image	Note
TS27: cochlear ganglion	Present		2G Spiral ganglion	Robust expression in statoacoustic ganglion, including spiral ganglion.

Structure	Level	Pattern	Image	Note
TS27: macula of saccule	Absent		2G Macula/Crista	No expression in vestibule, including saccular macula.

Structure	Level	Pattern	Image	Note
TS20: brain ventricular layer	Present		3A	Expression limited to statoacoustic ganglion and sensory epithelium of vestibule and cochlea.
TS20: retina	Present		3A	Expression limited to statoacoustic ganglion and sensory epithelium of vestibule and cochlea.
TS20: trigeminal ganglion	Present		3A	Expression limited to statoacoustic ganglion and sensory epithelium of vestibule and cochlea.

Structure	Level	Pattern	Image	Note
TS20: cochlea epithelium	Present		3A Sa	Expression in sensory epithelium
TS20: saccule	Present		3A Sa	Expression in sensory epithelium

Structure	Level	Pattern	Image	Note
TS20: vestibulo-cochlear ganglion	Present		3A SAG	Expression in statoacoustic ganglion.

Structure	Level	Pattern	Image	Note
TS23: inner ear epithelium	Present	Regionally restricted	3B	Expression confined to statoacoustic ganglion and sensory epithelium of inner ear.
TS23: vestibulo-cochlear ganglion	Present	Regionally restricted	3B	Expression confined to statoacoustic ganglion and sensory epithelium of inner ear.

Structure	Level	Pattern	Image	Note
TS23: cochlea epithelium	Present	Regionally restricted	3B Co	Expression limited in neural lateral region of sensory epithelia of cochlea.

Structure	Level	Pattern	Image	Note
TS23: cochlear ganglion	Present		3B SG	Expression in spiral ganglion.

Structure	Level	Pattern	Image	Note
TS23: saccule	Present		3B SA	Expression in vestibular sensory epithelia.
TS23: vestibular component epithelium	Present		3B SA	Expression in vestibular sensory epithelia.

Structure	Level	Pattern	Image	Note
TS23: utricle	Present		3B Ut	Expression in vestibular sensory epithelia.
TS23: vestibular component epithelium	Present		3B Ut	Expression in vestibular sensory epithelia.

Structure	Level	Pattern	Image	Note
TS26: cochlea epithelium	Present		3C	Expression limited to spiral ganglion, vestibular ganglion and sensory epithelium of cochlea.
TS26: cochlear ganglion	Present		3C	Expression limited to spiral ganglion, vestibular ganglion and sensory epithelium of cochlea.
TS26: vestibular ganglion	Present		3C	Expression limited to spiral ganglion, vestibular ganglion and sensory epithelium of cochlea.

Structure	Level	Pattern	Image	Note
TS26: cochlea epithelium	Present	Regionally restricted	3C Co	Expression limited in neural lateral region of sensory epithelium.
TS26: organ of Corti	Present		3C Co	Clear expression in organ of Corti.

Structure	Level	Pattern	Image	Note
TS26: cochlear ganglion	Present		3C SG	Expression in spiral ganglion.

Structure	Level	Pattern	Image	Note
TS26: vestibular ganglion	Present		3C VG

Structure	Level	Pattern	Image	Note
TS27: vestibulo-cochlear ganglion	Present	Regionally restricted	3D	Specifically expressed in statoacoustic ganglion, including spiral and vestibular ganglia.

Structure	Level	Pattern	Image	Note
TS27: cochlea epithelium	Weak		3D Co	Relatively weak expression in sensory epithelium.

Structure	Level	Pattern	Image	Note
TS27: cochlear ganglion	Strong		3D SG	Robust expression in spiral ganglion.

Structure	Level	Pattern	Image	Note
TS27: vestibular ganglion	Strong		3D VG