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Gene Expression Data
Assay Details
Assay
Reference: J:238636 Fededa JP, et al., MicroRNA-34/449 controls mitotic spindle orientation during mammalian cortex development. EMBO J. 2016 Nov 15;35(22):2386-2398
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7482549
Gene symbol: Tubg2
Gene name: tubulin, gamma 2
Antibody: anti-gamma-tubulin
Assay notes: The specificity of the antibody used was not detailed; both family members have been annotated.
Results
Specimen 3K gamma-tubulin: embryonic day 14.0; Mir34atm1.1Aven/Mir34a+, Mirc21tm1.1Aven/Mirc21+, Mirc34tm1.1Cxu/Mirc34+ (more )
Note: Heterozygous littermate control mice were used but the specific genotype for specimen shown was not detailed. Triple labeled: green - Tubg1 and Tubg2; red - phospho-vimentin (assay MGI:7482544); magenta - phalloidin.
Structure Cell Type Level Pattern Image Note
TS22: neocortex forebrain radial glial cell Present 3K
Specimen not shown: embryonic day 14.0; Mir34atm1.1Aven/Mir34atm1.1Aven, Mirc21tm1.1Aven/Mirc21tm1.1Aven, Mirc34tm1.1Cxu/Mirc34tm1.1Cxu (more )
Note: Double (DKO) and triple (TKO) knockout littermates were grouped in this study. The specific genotype of the specimen shown was not reported. Embryos may or may not have expressed the Mir34a wild type allele.
Structure Cell Type Level Pattern Image Note
TS22: neocortex forebrain radial glial cell Present Regionally restricted Authors report radial glial cells have significantly reduced angle relative to ventricular surface compared with heterozygous littermates.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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