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Gene Expression Data
Assay Details
Assay
Reference: J:333869 Nourse JL, et al., Piezo1 regulates cholesterol biosynthesis to influence neural stem cell fate during brain development. J Gen Physiol. 2022 Oct 3;154(10)
Assay type: RNA in situ
MGI Accession ID: MGI:7481975
Gene symbol: Piezo1
Gene name: piezo-type mechanosensitive ion channel component 1
Probe: Mm-Piezo1 (400181)
Probe preparation: Antisense
Visualized with: Fluorescence
Assay notes: RNAscope assay.
Results
Specimen 2A - WT forebrain: embryonic day 10.5 (more )
Note: Triple labeled: yellow - Piezo1; magenta - Tubb3 (assay MGI:7481980); cyan - Hoechst.
Structure Level Pattern Image Note
TS17: forebrain Present Punctate 2A WT Forebrain Piezo1 , 2A WT Forebrain merge Expression spans the width of the neuroepithelium.
Specimen 2A - WT midbrain: embryonic day 10.5 (more )
Note: Triple labeled: yellow - Piezo1; magenta - Tubb3 (assay MGI:7481980); cyan - Hoechst.
Structure Level Pattern Image Note
TS17: midbrain Present Punctate 2A WT Midbrain Piezo1 , 2A WT Midbrain merge Expression spans the width of the neuroepithelium.
Specimen 2A/S1 - WT hindbrain: embryonic day 10.5 (more )
Note: Triple labeled: yellow - Piezo1; magenta - Tubb3 (assay MGI:7481980); cyan - Hoechst.
Structure Level Pattern Image Note
TS17: hindbrain Present Punctate S1_WT merge , S1_WT Piezo1 , 2A WT Hindbrain Piezo1 , 2A WT Hindbrain merge Expression spans the width of the neuroepithelium.
TS17: brain blood vessel Strong 2A WT Hindbrain merge , S1_WT merge , 2A WT Hindbrain Piezo1 , S1_WT Piezo1
Specimen 2A - KO midbrain: embryonic day 10.5; Piezo1Gt(RST343)Byg/Piezo1Gt(RST343)Byg (more )
Note: Triple labeled: yellow - Piezo1; magenta - Tubb3 (assay MGI:7481980); cyan - Hoechst.
Structure Level Pattern Image Note
TS17: midbrain Absent 2A KO Midbrain Piezo1 , 2A KO Midbrain merge
Specimen S1 - KO hindbrain: embryonic day 10.5; Piezo1Gt(RST343)Byg/Piezo1Gt(RST343)Byg (more )
Note: Triple labeled: yellow - Piezo1; magenta - Tubb3 (assay MGI:7481980); cyan - Hoechst.
Structure Level Pattern Image Note
TS17: hindbrain Absent S1_KO Piezo1 , S1_KO merge
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory