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Gene Expression Data
Assay Details
Assay
Reference: J:286586 Vyssokikh MY, et al., Mild depolarization of the inner mitochondrial membrane is a crucial component of an anti-aging program. Proc Natl Acad Sci U S A. 2020 Mar 24;117(12):6491-6501
Assay type: RT-PCR
MGI Accession ID: MGI:7469897
Gene symbol: Hk2
Gene name: hexokinase 2
Probe: HK-II-pF, HK-II-pR
Probe preparation: labelled with SYBR green
Assay notes: Quantitative RT-PCR. Relative expression was normalized to beta-actin.
Results
Image: 9B ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (b) Amount Genetic BackgroundMutant Allele(s)Sex
Embryo skel. musc. E18.0 (a) TS26: skeletal muscle Present 0.5 µg; total RNA (C57BL/6 x CBA)F1 Not Specified
Embryo brain E18.0 (a) TS26: brain Present 0.5 µg; total RNA (C57BL/6 x CBA)F1 Not Specified
Embryo liver E18.0 (a) TS26: liver Present 0.5 µg; total RNA (C57BL/6 x CBA)F1 Not Specified
Embryo kidney E18.0 (a) TS26: metanephros Present 0.5 µg; total RNA (C57BL/6 x CBA)F1 Not Specified
Embryo heart E18.0 (a) TS26: heart Present 0.5 µg; total RNA (C57BL/6 x CBA)F1 Not Specified
Embryo diaphragm E18.0 (a) TS26: diaphragm Present 0.5 µg; total RNA (C57BL/6 x CBA)F1 Not Specified
Embryo lung E18.0 (a) TS26: lung Present 0.5 µg; total RNA (C57BL/6 x CBA)F1 Not Specified
Embryo spleen E18.0 (a) TS26: spleen Present 0.5 µg; total RNA (C57BL/6 x CBA)F1 Not Specified
3 month skel. musc. P m 3 TS28: skeletal muscle Present 0.5 µg; total RNA (C57BL/6 x CBA)F1 Not Specified
3 month brain P m 3 TS28: brain Present 0.5 µg; total RNA (C57BL/6 x CBA)F1 Not Specified
3 month liver P m 3 TS28: liver Ambiguous 0.5 µg; total RNA (C57BL/6 x CBA)F1 Not Specified
3 month kidney P m 3 TS28: metanephros Present 0.5 µg; total RNA (C57BL/6 x CBA)F1 Not Specified
3 month heart P m 3 TS28: heart Present 0.5 µg; total RNA (C57BL/6 x CBA)F1 Not Specified
3 month diaphragm P m 3 TS28: diaphragm Present 0.5 µg; total RNA (C57BL/6 x CBA)F1 Not Specified
3 month lung P m 3 TS28: lung Present 0.5 µg; total RNA (C57BL/6 x CBA)F1 Not Specified
3 month spleen P m 3 TS28: spleen Present 0.5 µg; total RNA (C57BL/6 x CBA)F1 Not Specified
12 month skel. musc. P m 12 TS28: skeletal muscle Present 0.5 µg; total RNA (C57BL/6 x CBA)F1 Not Specified
12 month brain P m 12 TS28: brain Present 0.5 µg; total RNA (C57BL/6 x CBA)F1 Not Specified
12 month liver P m 12 TS28: liver Ambiguous 0.5 µg; total RNA (C57BL/6 x CBA)F1 Not Specified
12 month kidney P m 12 TS28: metanephros Present 0.5 µg; total RNA (C57BL/6 x CBA)F1 Not Specified
12 month heart P m 12 TS28: heart Present 0.5 µg; total RNA (C57BL/6 x CBA)F1 Not Specified
12 month diaphragm P m 12 TS28: diaphragm Present 0.5 µg; total RNA (C57BL/6 x CBA)F1 Not Specified
12 month lung P m 12 TS28: lung Present 0.5 µg; total RNA (C57BL/6 x CBA)F1 Not Specified
12 month spleen P m 12 TS28: spleen Present 0.5 µg; total RNA (C57BL/6 x CBA)F1 Not Specified
24 month skel. musc. P m 24 TS28: skeletal muscle Present 0.5 µg; total RNA (C57BL/6 x CBA)F1 Not Specified
24 month brain P m 24 TS28: brain Present 0.5 µg; total RNA (C57BL/6 x CBA)F1 Not Specified
24 month liver P m 24 TS28: liver Ambiguous 0.5 µg; total RNA (C57BL/6 x CBA)F1 Not Specified
24 month kidney P m 24 TS28: metanephros Present 0.5 µg; total RNA (C57BL/6 x CBA)F1 Not Specified
24 month heart P m 24 TS28: heart Present 0.5 µg; total RNA (C57BL/6 x CBA)F1 Not Specified
24 month diaphragm P m 24 TS28: diaphragm Present 0.5 µg; total RNA (C57BL/6 x CBA)F1 Not Specified
24 month lung P m 24 TS28: lung Present 0.5 µg; total RNA (C57BL/6 x CBA)F1 Not Specified
24 month spleen P m 24 TS28: spleen Present 0.5 µg; total RNA (C57BL/6 x CBA)F1 Not Specified
30 month skel. musc. P m 30 TS28: skeletal muscle Present 0.5 µg; total RNA (C57BL/6 x CBA)F1 Not Specified
30 month brain P m 30 TS28: brain Present 0.5 µg; total RNA (C57BL/6 x CBA)F1 Not Specified
30 month liver P m 30 TS28: liver Ambiguous 0.5 µg; total RNA (C57BL/6 x CBA)F1 Not Specified
30 month kidney P m 30 TS28: metanephros Present 0.5 µg; total RNA (C57BL/6 x CBA)F1 Not Specified
30 month heart P m 30 TS28: heart Present 0.5 µg; total RNA (C57BL/6 x CBA)F1 Not Specified
30 month diaphragm P m 30 TS28: diaphragm Present 0.5 µg; total RNA (C57BL/6 x CBA)F1 Not Specified
30 month lung P m 30 TS28: lung Present 0.5 µg; total RNA (C57BL/6 x CBA)F1 Not Specified
30 month spleen P m 30 TS28: spleen Present 0.5 µg; total RNA (C57BL/6 x CBA)F1 Not Specified
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Expression was increased in skeletal muscle at 3 and 12 mo and strongly decreased thereafter. Among other tissues, only the lung showed a stable increase in hexokinase II mRNA up to 30 mo.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory