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Gene Expression Data
Assay Details
Assay
Reference: J:334839 Chuang CK, et al., The Role of SCL Isoforms in Embryonic Hematopoiesis. Int J Mol Sci. 2023 Mar 29;24(7)
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7466317
Gene symbol: Spn
Gene name: sialophorin
Antibody: anti-CD43 [DF-T1] (GTX73633)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 4A CD43 (lower): embryonic day 9.5 (more )
Note: Double labeled: red - Spn; green - Pecam1 (CD31; assay MGI:7466302).
Structure Cell Type Level Pattern Image Note
TS15: hematopoietic system hematopoietic stem cell Present Scattered 4A Merge (lower) , 4A CD43 (lower) Expression in dispersed cells in vessel cavities.
Specimen 4B CD43 (lower): embryonic day 9.5 (more )
Note: Double labeled: red - Spn; green - Pecam1 (CD31; assay MGI:7466302).
Structure Cell Type Level Pattern Image Note
TS15: hematopoietic system hematopoietic stem cell Present Scattered 4B CD43 (lower) , 4B Merge (lower) Expression in dispersed cells in vessel cavities.
Specimen 5A CD43 (lower): embryonic day 10.5 (more )
Note: Double labeled: red - Spn; green - Pecam1 (CD31; assay MGI:7466302).
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta endothelium Present Regionally restricted 5A CD 43 (lower) , 5A Merge (lower) Co-expression with Cd31 emerging from endothelial cell layer of aorta.
TS17: hematopoietic system hematopoietic stem cell Present Regionally restricted 5A Merge (lower) , 5A CD 43 (lower) Co-expression with Cd31 in a few cells in other cavities beyond aorta.
Specimen 5B CD43 (lower): embryonic day 10.5; Tal1em1Ckc/Tal1+ (more )
Note: Double labeled: red - Spn; green - Pecam1 (CD31; assay MGI:7466302).
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta endothelium Present Regionally restricted 5B CD43 (lower) , 5B Merge (lower) Co-expression with Cd31 emerging from endothelial cell layer of aorta.
TS17: hematopoietic system hematopoietic stem cell Present Regionally restricted 5B CD43 (lower) , 5B Merge (lower) Co-expression with Cd31 in a few cells in other cavities beyond aorta.
Specimen 5C CD43 (lower): embryonic day 10.5; Tal1em1Ckc/Tal1em1Ckc (more )
Note: Double labeled: red - Spn; green - Pecam1 (CD31; assay MGI:7466302).
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta endothelium Absent 5C CD43 (lower) , 5C Merge (lower) No expression in endothelial cell layer of aorta.
TS17: hematopoietic system hematopoietic stem cell Present Regionally restricted 5C Merge (lower) , 5C CD43 (lower) Co-expression with Cd31 in a few cells in other cavities beyond aorta.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory