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Gene Expression Data
Assay Details
Assay
Reference: J:334839 Chuang CK, et al., The Role of SCL Isoforms in Embryonic Hematopoiesis. Int J Mol Sci. 2023 Mar 29;24(7)
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7466302
Gene symbol: Pecam1
Gene name: platelet/endothelial cell adhesion molecule 1
Antibody: anti-CD31 (CM303)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Assay notes: Two CD31 antibodies were described, but the one used in this assay was not detailed. Anti-CD31 (GTX130274) MGI:7466288 may have been used in this assay.
Results
Specimen 2A CD31: embryonic day 10.0 (more )
Note: Double labeled: green - Pecam1; red - Cd34 (assay MGI:7466310).
Structure Cell Type Level Pattern Image Note
TS16: yolk sac blood vessel blood vessel endothelial cell Present Regionally restricted 2A
TS16: dorsal aorta endothelium aortic endothelial cell Present 2A
Specimen 2B CD31: embryonic day 10.0 (more )
Note: Double labeled: green - Pecam1; red - Cd34 (assay MGI:7466310).
Structure Level Pattern Image Note
TS16: yolk sac Present Regionally restricted 2B CD31/CD34 , 2B CD31 , 2B CD31/CD34/DiD Expression in defined tubular structures in yolk sac.
Specimen 4A CD31 (upper): embryonic day 9.5 (more )
Note: Double labeled: green - Pecam1; red - Cd34 (assay MGI:7466310).
Structure Cell Type Level Pattern Image Note
TS15: dorsal aorta endothelium Absent 4A Merge (upper) , 4A CD31 (upper) No expression in endothelial layer of aorta at E9.5.
TS15: hematopoietic system hematopoietic stem cell Present Scattered 4A CD31 (upper) , 4A Merge (upper) Expression in dispersed cells in vessel cavities.
Specimen 4A CD31 (lower): embryonic day 9.5 (more )
Note: Double labeled: green - Pecam1; red - Spn (Cd43; assay MGI:7466317).
Structure Cell Type Level Pattern Image Note
TS15: dorsal aorta endothelium Absent 4A CD31 (lower) , 4A Merge (lower) No expression in endothelial layer of aorta at E9.5.
TS15: hematopoietic system hematopoietic stem cell Present Scattered 4A Merge (lower) , 4A CD31 (lower) Expression in dispersed cells in vessel cavities.
Specimen 4B CD31 (upper): embryonic day 9.5 (more )
Note: Double labeled: green - Pecam1; red - Cd34 (assay MGI:7466310).
Structure Cell Type Level Pattern Image Note
TS15: hematopoietic system hematopoietic stem cell Present Scattered 4B Merge (upper) , 4B CD31 (upper) Expression in dispersed cells in vessel cavities.
Specimen 4B CD31 (lower): embryonic day 9.5 (more )
Note: Double labeled: green - Pecam1; red - Spn (Cd43; assay MGI:7466317).
Structure Cell Type Level Pattern Image Note
TS15: hematopoietic system hematopoietic stem cell Present Scattered 4B Merge (lower) , 4B CD31 (lower) Expression in dispersed cells in vessel cavities.
Specimen 4C CD31 (lower): embryonic day 9.5 (more )
Note: Double labeled: green - Pecam1; red - Kit (assay MGI:7466323).
Structure Cell Type Level Pattern Image Note
TS15: hematopoietic system hematopoietic stem cell Present Scattered 4C CD31 (lower) , 4C Merge (lower) Expression in dispersed cells in vessel cavities.
Specimen 5A CD31 (upper): embryonic day 10.5 (more )
Note: Double labeled: green - Pecam1; red - Cd34 (assay MGI:7466310).
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta endothelium Present Regionally restricted 5A Merge (upper) , 5A CD 31 (upper) Co-expression with Cd34 in cells emerging from endothelial cell layer of aorta.
TS17: hematopoietic system hematopoietic stem cell Present Regionally restricted 5A Merge (upper) , 5A CD 31 (upper) Co-expression with Cd34 in cells in other lumens.
Specimen 5A CD31 (lower): embryonic day 10.5 (more )
Note: Double labeled: green - Pecam1; red - Spn (Cd43; assay MGI:7466317).
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta endothelium Present Regionally restricted 5A Merge (lower) , 5A CD31 (lower) Co-expression with Cd43 (Spn) emerging from endothelial cell layer of aorta.
TS17: hematopoietic system hematopoietic stem cell Present Regionally restricted 5A CD31 (lower) , 5A Merge (lower) Co-expression with Cd43 in cells in other lumens
Specimen 5B CD31 (upper): embryonic day 10.5; Tal1em1Ckc/Tal1+ (more )
Note: Double labeled: green - Pecam1; red - Cd34 (assay MGI:7466310).
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta endothelium Present Regionally restricted 5B CD31 (upper) , 5B Merge (upper) Co-expression with Cd34 emerging from endothelial cell layer of aorta.
TS17: hematopoietic system hematopoietic stem cell Present Regionally restricted 5B CD31 (upper) , 5B Merge (upper) Co-expression with Cd34 in cells in other lumens
Specimen 5B CD31 (lower): embryonic day 10.5; Tal1em1Ckc/Tal1+ (more )
Note: Double labeled: green - Pecam1; red - Spn (Cd43; assay MGI:7466317).
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta endothelium Present Regionally restricted 5B Merge (lower) , 5B CD31 (lower) Co-expression with Cd43 (Spn) emerging from endothelial cell layer of aorta.
TS17: hematopoietic system hematopoietic stem cell Present Regionally restricted 5B Merge (lower) , 5B CD31 (lower) Co-expression with Cd43 in cells in other lumens.
Specimen 5C CD31 (upper): embryonic day 10.5; Tal1em1Ckc/Tal1em1Ckc (more )
Note: Double labeled: green - Pecam1; red - Cd34 (assay MGI:7466310).
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta endothelium Present Regionally restricted 5C CD31 (upper) , 5C Merge (upper) Expression in cells not expressing Cd43 (Spn) in endothelial cell layer of aorta.
TS17: hematopoietic system hematopoietic stem cell Present Regionally restricted 5C CD31 (upper) , 5C Merge (upper) Co-expression with Cd34 in a few cells in other cavities beyond aorta.
Specimen 5C CD31 (lower): embryonic day 10.5; Tal1em1Ckc/Tal1em1Ckc (more )
Note: Double labeled: green - Pecam1; red - Spn (Cd43; assay MGI:7466317).
Structure Cell Type Level Pattern Image Note
TS17: dorsal aorta endothelium Present Regionally restricted 5C CD31 (lower) , 5C Merge (lower) Expression in cells not expressing Cd43 (Spn) in endothelial cell layer of aorta.
TS17: hematopoietic system hematopoietic stem cell Present Regionally restricted 5C Merge (lower) , 5C CD31 (lower) Co-expression with Cd43 in a few cells in other cavities beyond aorta.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
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