Hbp1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:247327 Spiller CM, et al., Mice Lacking Hbp1 Function Are Viable and Fertile. PLoS One. 2017;12(1):e0170576
Assay type:	RT-PCR
MGI Accession ID:	MGI:7464893
Gene symbol:	Hbp1
Gene name:	high mobility group box transcription factor 1

Probe:	Fl_qPCR-pF, Fl_qPCR-pR
Probe preparation:	labelled with SYBR green
Assay notes:	Quantitative RT-PCR. Relative expression was normalized to 18S RNA. The primer set used in this assay is designed to recognize the full length transcript.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	115 bp	Amount	Genetic Background	Mutant Allele(s)	Sex
Wildtype XY	E13.5	TS21: testis	Present	Not Specified; total RNA	Not Specified		Male
Wildtype XX	E13.5	TS21: ovary	Present	Not Specified; total RNA	Not Specified		Female
W^e/W^e XY	E13.5	TS21: testis	Present (a)	Not Specified; total RNA	involves: 101 * C3H * Quackenbush Swiss	Kit^W-e/Kit^W-e	Male
W^e/W^e XX	E13.5	TS21: ovary	Present (b)	Not Specified; total RNA	involves: 101 * C3H * Quackenbush Swiss	Kit^W-e/Kit^W-e	Female

Notes:
(a) Expression levels are significantly reduced in the male mutants.
(b) Expression levels are not significantly reduced in the female mutants, suggesting there is more somatic expression in the females.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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