Gja8 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:277247 Vetrivel S, et al., Mutation in the mouse histone gene Hist2h3c1 leads to degeneration of the lens vesicle and severe microphthalmia. Exp Eye Res. 2019 Apr 13;
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7461916
Gene symbol:	Gja8
Gene name:	gap junction protein, alpha 8

Antibody:	anti-Connexin 50 (PA511644)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

Specimen 7 E11.5 C3H wild type: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: lens vesicle	Present	Widespread	7_E11.5 C3H wild type	Expressing covering entire lens vesicle.

Specimen 7 E11.5 Aey69 mutant: embryonic day 11.5; Gja8^Aey69/Gja8^Aey69, H3c14^Aey69/H3c14^Aey69 (more

)

Structure	Level	Pattern	Image	Note
TS19: lens vesicle	Absent		7_E11.5 Aey69 mutant

Specimen 7 E11.5 aphakia mutant: embryonic day 11.5; Pitx3^ak/Pitx3^ak (more

)

Structure	Level	Pattern	Image	Note
TS19: lens vesicle	Present	Regionally restricted	7_E11.5 aphakia mutant	Highly irregular expression restrictred to one part of lens vesicle.

Specimen 7 E12.5 C3H wild type: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: lens vesicle	Present	Regionally restricted	7_E12.5 C3H wild type	Expression more restricted to region beneath future lens epithelial layer.

Specimen 7 E12.5 Aey69 mutant: embryonic day 12.5; Gja8^Aey69/Gja8^Aey69, H3c14^Aey69/H3c14^Aey69 (more

)

Structure	Level	Pattern	Image	Note
TS20: lens vesicle	Absent		7_E12.5 Aey69 mutant

Specimen 7 E12.5 aphakia mutant: embryonic day 12.5; Pitx3^ak/Pitx3^ak (more

)

Structure	Level	Pattern	Image	Note
TS20: lens vesicle	Present	Regionally restricted	7_E12.5 aphakia mutant	Continued one-sided expression in disorganlized lens structure.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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