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Gene Expression Data
Assay Details
Assay
Reference: J:328290 Nakagawa T, et al., A multistate stem cell dynamics maintains homeostasis in mouse spermatogenesis. Cell Rep. 2021 Oct 19;37(3):109875
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7461477
Gene symbol: Gfra1
Gene name: glial cell line derived neurotrophic factor family receptor alpha 1
Antibody: anti-GFR alpha-1 (AF560)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 1G: postnatal adult (more )
Note: Triple labeled: cyan - Gfra1; magenta - Plvap (assay MGI:7461459); green - Sox3 (assay MGI:7461480).
Structure Level Pattern Image Note
TS28: seminiferous tubule Present Regionally restricted 1G MERGE , 1G GFRa1 Expression is seen in the least differentiated spermatogonia.
TS28: spermatogonium Present Regionally restricted 1G MERGE , 1G GFRa1 Expression is seen in the least differentiated spermatogonia.
Specimen S2D: postnatal adult; Ttm1(cre/ERT2)Hssk/T+ (more )
Note: Multi-labeled: cyan - Gfra1 (with Pdx1); magenta - Plvap (assay MGI:7461459); green - T (assay MGI:7461503); yellow - Pdx1 (assay MGI:7461502).
Structure Level Pattern Image Note
TS28: seminiferous tubule Present Regionally restricted S2D GFRa1 plus PDX1 , S2D Merge Expression is seen in the least differentiated spermatogonia.
TS28: spermatogonium Present Regionally restricted S2D GFRa1 plus PDX1 , S2D Merge Expression is seen in the least differentiated spermatogonia.
Specimen S3B: postnatal adult; Plvaptm1.1(cre/ERT2)Tng/Plvap+ (more )
Note: Triple labeled: cyan - Gfra1; green - Plvap reporter (assay MGI:7461237); magenta - Plvap protein (assay MGI:7461459).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted S3B MERGE , S3B GFRa1
TS28: testis Present Regionally restricted S3B MERGE , S3B GFRa1
Specimen S3C: postnatal adult (more )
Note: Wild type. Double labeled: cyan - Gfra1; magenta - Plvap protein (assay MGI:7461459).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted S3C MERGE , S3C Gfra1
TS28: testis Present Regionally restricted S3C MERGE , S3C Gfra1
Specimen S3D: postnatal adult; Plvaptm1.1(cre/ERT2)Tng/Plvap+ (more )
Note: Triple labeled: cyan - Gfra1; green - Plvap reporter (assay MGI:7461237); magenta - Plvap protein (assay MGI:7461459).
Structure Level Pattern Image Note
TS28: seminiferous tubule Present Regionally restricted S3D MERGE , S3D GFRa1
TS28: spermatogonium Present Regionally restricted S3D MERGE , S3D GFRa1
Specimen S3E: postnatal adult (more )
Note: Wild type. Double labeled: cyan - Gfra1; magenta - Plvap protein (assay MGI:7461459).
Structure Level Pattern Image Note
TS28: seminiferous tubule Present Regionally restricted S3E GFRa1 , S3E MERGE
TS28: spermatogonium Present Regionally restricted S3E GFRa1 , S3E MERGE
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
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