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Gene Expression Data
Assay Details
Assay
Reference: J:178176 Laforest B, et al., GATA5 interacts with GATA4 and GATA6 in outflow tract development. Dev Biol. 2011 Oct 15;358(2):368-78
Assay type: RT-PCR
MGI Accession ID: MGI:7461185
Gene symbol: Gata5
Gene name: GATA binding protein 5
Probe: Gata5-pF, Gata5-pR
Probe preparation: labelled with SYBR green
Assay notes: Quantitative RT-PCR. Relative expression was normalized to Gapdh. The authors did not specify which primers were used and the following primers could have been used as well: MGI:7461149.
Results
Image: 4B ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
WT E12.5 TS20: heart Present 2 µg; total RNA involves: C57BL/6 Not Specified
G4+/- E12.5 TS20: heart Present 2 µg; total RNA involves: 129S7/SvEvBrd * C57BL/6 Gata4tm1Eno/Gata4+Not Specified
G5+/- E12.5 TS20: heart Present 2 µg; total RNA involves: C57BL/6 Gata5tm1.2Nemr/Gata5+Not Specified
G4/G5 E12.5 TS20: heart Present (a) 2 µg; total RNA involves: 129S7/SvEvBrd * C57BL/6 Gata4tm1Eno/Gata4+, Gata5tm1.2Nemr/Gata5+Not Specified
Notes:
(a) 1 - In this mutant transcripts were reduced by 60% compared to wild-type littermates.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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