Antibody:	anti-STk2
Detection system:	Secondary antibody coupled to Texas Red

Structure	Level	Pattern	Image	Note
TS22: skeletal muscle	Present	Regionally restricted	1A	Expression is in myotubes distributed throughout the neuraxis, including the trunk and limb, as well as various cervical and facial muscles anlagen.
TS22: cardiac muscle tissue	Absent		1A
TS22: smooth muscle tissue	Absent		1A

Structure	Level	Pattern	Image	Note
TS22: skeletal muscle	Present		1B	Expression is in myotubes distributed throughout the neuraxis, including the trunk and limb, as well as various cervical and facial muscles anlagen.
TS22: cardiac muscle tissue	Absent		1B
TS22: smooth muscle tissue	Absent		1B

Structure	Level	Pattern	Image	Note
TS25: skeletal muscle	Present	Regionally restricted	1C second , 1C fourth	Expression is in a subpopulation of myotubes with migratory morphology in the caudal muscle anlagen.

Structure	Level	Pattern	Image	Note
TS25: cranial musculature	Present	Regionally restricted	1D fourth , 1D second	Expression is in a subpopulation of myotubes in the occipital muscle anlagen.