Lipe Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:236180 Zhou H, et al., Berardinelli-Seip Congenital Lipodystrophy 2/Seipin Is Not Required for Brown Adipogenesis but Regulates Brown Adipose Tissue Development and Function. Mol Cell Biol. 2016 Aug 1;36(15):2027-38
Assay type:	Western blot
MGI Accession ID:	MGI:7447323
Gene symbol:	Lipe
Gene name:	lipase, hormone sensitive

Antibody:

anti-phospho-HSL (Ser563) (4139)

Results

Image: 10B pHSL

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Genetic Background	Mutant Allele(s)	Sex	Note
Mo.3 C	P m 3	TS28: interscapular fat pad	Present	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Bscl2^tm1Lchan/Bscl2^tm1Lchan	Male
Mo.3 C	P m 3	TS28: interscapular fat pad	Present	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Bscl2^tm1Lchan/Bscl2^tm1Lchan	Male
Mo.3 K	P m 3	TS28: interscapular fat pad	Strong (a)	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Bscl2^tm1Lchan/Bscl2^tm1Lchan, Myf5^tm1(cre)Mrc/Myf5⁺	Male	Conditional mutant.
Mo.3 K	P m 3	TS28: interscapular fat pad	Strong (a)	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Bscl2^tm1Lchan/Bscl2^tm1Lchan, Myf5^tm1(cre)Mrc/Myf5⁺	Male	Conditional mutant.
Mo.4 C	P m 4	TS28: interscapular fat pad	Present	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Bscl2^tm1Lchan/Bscl2^tm1Lchan	Male
Mo.4 C	P m 4	TS28: interscapular fat pad	Present	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Bscl2^tm1Lchan/Bscl2^tm1Lchan	Male
Mo.4 K	P m 4	TS28: interscapular fat pad	Present (b)	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Bscl2^tm1Lchan/Bscl2^tm1Lchan, Myf5^tm1(cre)Mrc/Myf5⁺	Male	Conditional mutant.
Mo.4 K	P m 4	TS28: interscapular fat pad	Present (b)	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Bscl2^tm1Lchan/Bscl2^tm1Lchan, Myf5^tm1(cre)Mrc/Myf5⁺	Male	Conditional mutant.

Notes:
(a) Authors report cAMP/PKA-mediated HSL phosphorylation remained elevated at 3 months.
(b) Authors report cAMP/PKA-mediated HSL phosphorylation was normalized by 4 months.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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