Lipe Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:236180 Zhou H, et al., Berardinelli-Seip Congenital Lipodystrophy 2/Seipin Is Not Required for Brown Adipogenesis but Regulates Brown Adipose Tissue Development and Function. Mol Cell Biol. 2016 Aug 1;36(15):2027-38
Assay type:	Western blot
MGI Accession ID:	MGI:7447314
Gene symbol:	Lipe
Gene name:	lipase, hormone sensitive

Antibody:

anti-phospho-HSL (Ser563) (4139)

Results

Image: 6B pHSL

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Genetic Background	Mutant Allele(s)	Sex	Note
E16.5 C	E16.5	TS24: brown fat	Absent (a)	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Bscl2^tm1Lchan/Bscl2^tm1Lchan	Not Specified
E16.5 K	E16.5	TS24: brown fat	Absent	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Bscl2^tm1Lchan/Bscl2^tm1Lchan, Myf5^tm1(cre)Mrc/Myf5⁺	Not Specified	Conditional mutant.
E18.5 C	E18.5	TS26: brown fat	Trace (b)	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Bscl2^tm1Lchan/Bscl2^tm1Lchan	Not Specified
E18.5 K	E18.5	TS26: brown fat	Present (c)	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Bscl2^tm1Lchan/Bscl2^tm1Lchan, Myf5^tm1(cre)Mrc/Myf5⁺	Not Specified	Conditional mutant.
P0 C	P0	TS27: brown fat	Strong (d)	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Bscl2^tm1Lchan/Bscl2^tm1Lchan	Not Specified
P0 K	P0	TS27: brown fat	Strong (e)	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Bscl2^tm1Lchan/Bscl2^tm1Lchan, Myf5^tm1(cre)Mrc/Myf5⁺	Not Specified	Conditional mutant.
P2 C	P2	TS27: brown fat	Present	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Bscl2^tm1Lchan/Bscl2^tm1Lchan	Not Specified
P2 K	P2	TS27: brown fat	Strong (e)	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Bscl2^tm1Lchan/Bscl2^tm1Lchan, Myf5^tm1(cre)Mrc/Myf5⁺	Not Specified	Conditional mutant.
P5 C	P5	TS28: brown fat	Present (f)	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Bscl2^tm1Lchan/Bscl2^tm1Lchan	Not Specified
P5 K	P5	TS28: brown fat	Strong (e)	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	Bscl2^tm1Lchan/Bscl2^tm1Lchan, Myf5^tm1(cre)Mrc/Myf5⁺	Not Specified	Conditional mutant.

Notes:
(a) Expression undetectable at E16.5.
(b) Very low expression at E18.5.
(c) Enhanced expression detected at E18.5.
(d) Expression sharply activated upon birth.
(e) Authors report cAMP/PKA-mediated phosphorylated overtly potentiated between P0 and P5.
(f) Expression returned to basal levels by P5.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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