Gene Expression Data
Assay Details
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Assay
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Reference:
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J:37463
Farinas I, et al., Lack of neurotrophin-3 results in death of spinal sensory neurons and premature differentiation of their precursors. Neuron. 1996 Dec;17(6):1065-78
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:7439190
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Gene symbol:
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Ntf3
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Gene name:
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neurotrophin 3
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Reporter:
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lacZ
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Direct Detection
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Results
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Specimen
5A:
embryonic day 10.5; Ntf3tm1Lfr/Ntf3 +
(more  )
Note: Double labeled: blue - Ntf3 reporter; brown - Tubb3 (assay MGI:7439195).
Specimen
5A:
(close  )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Ntf3tm1Lfr/Ntf3+
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Age:
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embryonic day 10.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: blue - Ntf3 reporter; brown - Tubb3 (assay MGI:7439195).
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Specimen
5B:
embryonic day 11.5; Ntf3tm1Lfr/Ntf3 +
(more )
Specimen
5B:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Ntf3tm1Lfr/Ntf3+
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Age:
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embryonic day 11.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Regionally restricted |
5B
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Expression is in the proximal part.
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| TS19: hindlimb bud |
Present |
Regionally restricted |
5B
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Expression is in the proximal part.
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| TS19: embryo |
Present |
Regionally restricted |
5B
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Expression extends caudally.
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Specimen
5C:
embryonic day 11.5; Ntf3tm1Lfr/Ntf3 +
(more )
Specimen
5C:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Ntf3tm1Lfr/Ntf3+
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Age:
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embryonic day 11.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: neural tube basal columns |
Strong |
Regionally restricted |
5C
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Expression is as two longitudinal stripes of staining that correspond to the lateral motor columns and is stronger at the level of the forelimb.
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Specimen
5D:
embryonic day 11.5; Ntf3tm1Lfr/Ntf3 +
(more )
Note: Double labeled: blue - Ntf3 reporter; brown - Tubb3 (assay MGI:7439195).
Specimen
5D:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Ntf3tm1Lfr/Ntf3+
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Age:
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embryonic day 11.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: blue - Ntf3 reporter; brown - Tubb3 (assay MGI:7439195).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
Regionally restricted |
5D
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Expression is in the proximal part.
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Specimen
5E:
embryonic day 10.5; Ntf3tm1Lfr/Ntf3 +
(more )
Specimen
5E:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Ntf3tm1Lfr/Ntf3+
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Age:
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embryonic day 10.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5F:
embryonic day 11.5; Ntf3tm1Lfr/Ntf3 +
(more )
Note: Double labeled: blue - Ntf3 reporter; brown - Nefm (assay MGI:7439188).
Specimen
5F:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Ntf3tm1Lfr/Ntf3+
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Age:
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embryonic day 11.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: blue - Ntf3 reporter; brown - Nefm (assay MGI:7439188).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Very strong |
Regionally restricted |
5F
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Expression is in areas where sensory and motor axons are actively growing. Expression is strongest surrounding the branch projecting dorsally toward the skin. Absent in the most distal part of the limb.
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| TS19: neural tube basal columns |
Strong |
Regionally restricted |
5F
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Expression is in the lateral motor column.
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Specimen
5G:
embryonic day 13.5; Ntf3tm1Lfr/Ntf3 +
(more )
Note: Double labeled: blue - Ntf3 reporter; brown - Tubb3 (assay MGI:7439195).
Specimen
5G:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Ntf3tm1Lfr/Ntf3+
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Ntf3 reporter; brown - Tubb3 (assay MGI:7439195).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: skin |
Present |
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5G
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| TS21: muscle tissue |
Present |
Regionally restricted |
5G
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Expression is in some muscles in the trunk.
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| TS21: forelimb mesenchyme |
Present |
Regionally restricted |
5G
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Expression is in association with the growing tips of the sensory-motor projection.
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Specimen
5H:
embryonic day 15.5; Ntf3tm1Lfr/Ntf3 +
(more )
Note: Double labeled: blue - Ntf3 reporter; brown - Tubb3 (assay MGI:7439195).
Specimen
5H:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Ntf3tm1Lfr/Ntf3+
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Cryosection
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Note:
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Double labeled: blue - Ntf3 reporter; brown - Tubb3 (assay MGI:7439195).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: forelimb mesenchyme |
Present |
Regionally restricted |
5H
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Expression is at the sites of axonal projections and is strong in the tip and is downregulated in the proximal area.
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Specimen
6A:
embryonic day 11.5; Ntf3tm1Lfr/Ntf3 +
(more )
Note: Double labeled: blue - Ntf3 reporter; brown - Nefm (assay MGI:7439188).
Specimen
6A:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Ntf3tm1Lfr/Ntf3+
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Age:
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embryonic day 11.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: blue - Ntf3 reporter; brown - Nefm (assay MGI:7439188).
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Specimen
6B:
embryonic day 11.5; Ntf3tm1Lfr/Ntf3 +
(more )
Note: Double labeled: blue - Ntf3 reporter; brown - Nefm (assay MGI:7439188).
Specimen
6B:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Ntf3tm1Lfr/Ntf3+
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Age:
|
embryonic day 11.5
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|
Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
|
4% Paraformaldehyde
|
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Note:
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Double labeled: blue - Ntf3 reporter; brown - Nefm (assay MGI:7439188).
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Specimen
6C:
embryonic day 15.5; Ntf3tm1Lfr/Ntf3 +
(more )
Note: Double labeled: blue - Ntf3 reporter; brown - Nefm (assay MGI:7439188).
Specimen
6C:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Ntf3tm1Lfr/Ntf3+
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: blue - Ntf3 reporter; brown - Nefm (assay MGI:7439188).
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Specimen
6D:
embryonic day 13.5; Ntf3tm1Lfr/Ntf3 +
(more )
Note: Double labeled: blue - Ntf3 reporter; brown - Nefm (assay MGI:7439188).
Specimen
6D:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Ntf3tm1Lfr/Ntf3+
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: blue - Ntf3 reporter; brown - Nefm (assay MGI:7439188).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: epidermis |
Present |
Regionally restricted |
6D
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Expression is in axons that invade the epidermis.
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| TS21: dermis |
Absent |
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6D
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Specimen
6E:
embryonic day 17.5; Ntf3tm1Lfr/Ntf3 +
(more )
Note: Double labeled: blue - Ntf3 reporter; brown - Nefm (assay MGI:7439188).
Specimen
6E:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Ntf3tm1Lfr/Ntf3+
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Age:
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embryonic day 17.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: blue - Ntf3 reporter; brown - Nefm (assay MGI:7439188).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS25: skeletal muscle |
myoblast |
Present |
Regionally restricted |
6E
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Expression is in myoblasts and is absent in muscle fibers.
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| TS25: dermis |
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Present |
Regionally restricted |
6E
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Expression is restricted to a subset of innervated muscles.
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Specimen
6F:
embryonic day 15.5; Ntf3tm1Lfr/Ntf3 +
(more )
Note: Double labeled: blue - Ntf3 reporter; brown - Nefm (assay MGI:7439188).
Specimen
6F:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Ntf3tm1Lfr/Ntf3+
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: blue - Ntf3 reporter; brown - Nefm (assay MGI:7439188).
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Specimen
6G:
embryonic day 17.5; Ntf3tm1Lfr/Ntf3 +
(more )
Note: Double labeled: blue - Ntf3 reporter; brown - Nefm (assay MGI:7439188).
Specimen
6G:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Ntf3tm1Lfr/Ntf3+
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Age:
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embryonic day 17.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: blue - Ntf3 reporter; brown - Nefm (assay MGI:7439188).
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Specimen
not shown:
embryonic day 9.5; Ntf3tm1Lfr/Ntf3 +
(more )
Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Ntf3tm1Lfr/Ntf3+
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Age:
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embryonic day 9.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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