Crb3 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:237224 Kim JY, et al., Yap is essential for retinal progenitor cell cycle progression and RPE cell fate acquisition in the developing mouse eye. Dev Biol. 2016 Nov 15;419(2):336-347
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7438260
Gene symbol:	Crb3
Gene name:	crumbs family member 3

Antibody:	Anti-Crb3
Detection system:	Secondary antibody coupled to Cy5
Assay notes:	The specificity of the antibody used was not detailed; all family members have been annotated.

Results

5B ©

Specimen 5B: embryonic day 15.5; Yap1^tm1.1Eno/Yap1^tm1.1Eno (more

)

Structure	Level	Pattern	Image	Note
TS23: neural retina	Present	Regionally restricted	5B	Expressed in outer limiting membrane in the apical surface.

5D ©

Specimen 5D: embryonic day 15.5; Tg(rx3-icre)1Mjam/0, Yap1^tm1.1Eno/Yap1^tm1.1Eno (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS23: neural retina	Present	Regionally restricted	5D	Abrupt discontinuation of primitive outer limiting membrane at the apical surface.

5E ©

5E1 ©

5E3 ©

Specimen 5E: embryonic day 16.5; Tg(rx3-icre)1Mjam/0, Yap1^tm1.1Eno/Yap1^tm1.1Eno (more

)
Note: Conditional mutant. Double labeled: red - pan-Crb; green - Yap1 (assay MGI:7437575).

Structure	Level	Pattern	Image	Note
TS24: neural retina	Present	Regionally restricted	5E3 , 5E , 5E1	The area showing a break in the presumptive outer limiting membrane correlated with a decrease in Yap1 expression.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 05/07/2024 MGI 6.23