Gene Expression Data
Assay Details
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Assay
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Reference:
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J:333149
Zhao T, et al., Sertoli cell PUMILIO proteins modulate mouse testis size through translational control of cell cycle regulators. Biol Reprod. 2022 Jul 25;107(1):135-147
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7437573
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Gene symbol:
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Pum1
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Gene name:
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pumilio RNA-binding family member 1
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Antibody:
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anti-Pumilio1 {EPR3795] (ab92545)
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Assay notes:
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Fixation was with Hartman's fixative.
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Results
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Specimen
1A E17.5 Control:
(close  )
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Genetic Background:
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involves: 129 * C57BL/6 * SJL
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Mutant Allele(s):
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Pum1tm1.1Eyx/Pum1tm1.1Eyx
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Age:
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embryonic day 17.5
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Sex:
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Male
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS25: germ cell of testis |
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Present |
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1A E17.5 Control
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Expression is seen in spermatocytes.
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| TS25: Sertoli cell |
Sertoli cell |
Strong |
Regionally restricted |
1A E17.5 Control
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Highly expressed in cytoplasm of Sertoli cells.
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Specimen
1A E17.5 AcP1KO:
(close )
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Genetic Background:
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involves: 129 * C57BL/6 * SJL
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Mutant Allele(s):
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Plekha5Tg(AMH-cre)1Flor/0
Pum1tm1.1Eyx/Pum1tm1.1Eyx
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Age:
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embryonic day 17.5
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Sex:
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Male
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Conditional mutant.
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Specimen
1A P3 Control:
(close )
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Genetic Background:
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involves: 129 * C57BL/6 * SJL
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Mutant Allele(s):
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Pum1tm1.1Eyx/Pum1tm1.1Eyx
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Age:
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postnatal day 3
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Sex:
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Male
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: spermatocyte |
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Present |
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1A P3 Control
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| TS27: Sertoli cell |
Sertoli cell |
Strong |
Regionally restricted |
1A P3 Control
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Highly expressed in cytoplasm of Sertoli cells.
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Specimen
1A P3 AcP1KO:
(close )
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Genetic Background:
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involves: 129 * C57BL/6 * SJL
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Mutant Allele(s):
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Plekha5Tg(AMH-cre)1Flor/0
Pum1tm1.1Eyx/Pum1tm1.1Eyx
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Age:
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postnatal day 3
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Sex:
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Male
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Conditional mutant.
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Specimen
1A P14 Control:
(close )
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Genetic Background:
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involves: 129 * C57BL/6 * SJL
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Mutant Allele(s):
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Pum1tm1.1Eyx/Pum1tm1.1Eyx
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Age:
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postnatal day 14
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Sex:
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Male
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: spermatocyte |
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Present |
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1A P14 Control
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| TS28: Sertoli cell |
Sertoli cell |
Present |
Regionally restricted |
1A P14 Control
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Expression less prominent in P14 and adult testes than fetal and neonatal P3.
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Specimen
1A P14 AcP1KO:
(close )
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Genetic Background:
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involves: 129 * C57BL/6 * SJL
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Mutant Allele(s):
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Plekha5Tg(AMH-cre)1Flor/0
Pum1tm1.1Eyx/Pum1tm1.1Eyx
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Age:
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postnatal day 14
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Sex:
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Male
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Conditional mutant.
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Specimen
1A Adult Control:
(close )
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Genetic Background:
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involves: 129 * C57BL/6 * SJL
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Mutant Allele(s):
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Pum1tm1.1Eyx/Pum1tm1.1Eyx
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: spermatocyte |
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Present |
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1A Adult Control
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| TS28: Sertoli cell |
Sertoli cell |
Present |
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1A Adult Control
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Expression less prominent in P14 and adult testes than fetal and neonatal P3.
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Specimen
1A Adult AcP1KO:
(close )
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Genetic Background:
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involves: 129 * C57BL/6 * SJL
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Mutant Allele(s):
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Plekha5Tg(AMH-cre)1Flor/0
Pum1tm1.1Eyx/Pum1tm1.1Eyx
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Conditional mutant.
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Specimen
S2 PUM P14 Control:
(close )
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Genetic Background:
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involves: 129 * C57BL/6 * SJL
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Mutant Allele(s):
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Pum1tm1.1Eyx/Pum1tm1.1Eyx
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Age:
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postnatal day 14
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Sex:
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Male
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: Sertoli cell |
Sertoli cell |
Present |
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S2_PUM1 P14 Control
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Specimen
S2 PUM1 P14 AcP1KO:
(close )
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Genetic Background:
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involves: 129 * C57BL/6 * SJL
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Mutant Allele(s):
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Plekha5Tg(AMH-cre)1Flor/0
Pum1tm1.1Eyx/Pum1tm1.1Eyx
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Age:
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postnatal day 14
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Sex:
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Male
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Conditional mutant.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: Sertoli cell |
Sertoli cell |
Absent |
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S2_PUM1 P14 AcP1KO
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Specimen
S2 PUM1 Adult Control:
(close )
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Genetic Background:
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involves: 129 * C57BL/6 * SJL
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Mutant Allele(s):
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Pum1tm1.1Eyx/Pum1tm1.1Eyx
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: Sertoli cell |
Sertoli cell |
Present |
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S2_PUM1 Adult Control
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Specimen
S2 PUM1 Adult AcP1KO:
(close )
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Genetic Background:
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involves: 129 * C57BL/6 * SJL
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Mutant Allele(s):
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Plekha5Tg(AMH-cre)1Flor/0
Pum1tm1.1Eyx/Pum1tm1.1Eyx
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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Conditional mutant.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: Sertoli cell |
Sertoli cell |
Absent |
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S2_PUM1 Adult AcP1KO
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)
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