Pum1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:333149 Zhao T, et al., Sertoli cell PUMILIO proteins modulate mouse testis size through translational control of cell cycle regulators. Biol Reprod. 2022 Jul 25;107(1):135-147
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7437573
Gene symbol:	Pum1
Gene name:	pumilio RNA-binding family member 1

Antibody:	anti-Pumilio1 {EPR3795] (ab92545)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	Fixation was with Hartman's fixative.

Results

Specimen 1A E17.5 Control: embryonic day 17.5; Pum1^tm1.1Eyx/Pum1^tm1.1Eyx (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS25: germ cell of testis		Present		1A E17.5 Control	Expression is seen in spermatocytes.
TS25: Sertoli cell	Sertoli cell	Strong	Regionally restricted	1A E17.5 Control	Highly expressed in cytoplasm of Sertoli cells.

Specimen 1A E17.5 AcP1KO: embryonic day 17.5; Plekha5^{Tg(AMH-cre)1Flor}/0, Pum1^tm1.1Eyx/Pum1^tm1.1Eyx (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS25: germ cell of testis		Present		1A E17.5 AcP1KO	Expression is seen in spermatocytes.
TS25: Sertoli cell	Sertoli cell	Absent		1A E17.5 AcP1KO

Specimen 1A P3 Control: postnatal day 3; Pum1^tm1.1Eyx/Pum1^tm1.1Eyx (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS27: spermatocyte		Present		1A P3 Control
TS27: Sertoli cell	Sertoli cell	Strong	Regionally restricted	1A P3 Control	Highly expressed in cytoplasm of Sertoli cells.

Specimen 1A P3 AcP1KO: postnatal day 3; Plekha5^{Tg(AMH-cre)1Flor}/0, Pum1^tm1.1Eyx/Pum1^tm1.1Eyx (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: spermatocyte		Present		1A P3 AcP1KO
TS27: Sertoli cell	Sertoli cell	Absent		1A P3 AcP1KO

Specimen 1A P14 Control: postnatal day 14; Pum1^tm1.1Eyx/Pum1^tm1.1Eyx (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: spermatocyte		Present		1A P14 Control
TS28: Sertoli cell	Sertoli cell	Present	Regionally restricted	1A P14 Control	Expression less prominent in P14 and adult testes than fetal and neonatal P3.

Specimen 1A P14 AcP1KO: postnatal day 14; Plekha5^{Tg(AMH-cre)1Flor}/0, Pum1^tm1.1Eyx/Pum1^tm1.1Eyx (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: spermatocyte		Present		1A P14 AcP1KO
TS28: Sertoli cell	Sertoli cell	Absent		1A P14 AcP1KO

Specimen 1A Adult Control: postnatal adult; Pum1^tm1.1Eyx/Pum1^tm1.1Eyx (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: spermatocyte		Present		1A Adult Control
TS28: Sertoli cell	Sertoli cell	Present		1A Adult Control	Expression less prominent in P14 and adult testes than fetal and neonatal P3.

Specimen 1A Adult AcP1KO: postnatal adult; Plekha5^{Tg(AMH-cre)1Flor}/0, Pum1^tm1.1Eyx/Pum1^tm1.1Eyx (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: spermatocyte		Present		1A Adult AcP1KO
TS28: Sertoli cell	Sertoli cell	Absent		1A Adult AcP1KO

Specimen S2 PUM P14 Control: postnatal day 14; Pum1^tm1.1Eyx/Pum1^tm1.1Eyx (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: Sertoli cell	Sertoli cell	Present		S2_PUM1 P14 Control

Specimen S2 PUM1 P14 AcP1KO: postnatal day 14; Plekha5^{Tg(AMH-cre)1Flor}/0, Pum1^tm1.1Eyx/Pum1^tm1.1Eyx (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: Sertoli cell	Sertoli cell	Absent		S2_PUM1 P14 AcP1KO

Specimen S2 PUM1 Adult Control: postnatal adult; Pum1^tm1.1Eyx/Pum1^tm1.1Eyx (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: Sertoli cell	Sertoli cell	Present		S2_PUM1 Adult Control

Specimen S2 PUM1 Adult AcP1KO: postnatal adult; Plekha5^{Tg(AMH-cre)1Flor}/0, Pum1^tm1.1Eyx/Pum1^tm1.1Eyx (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: Sertoli cell	Sertoli cell	Absent		S2_PUM1 Adult AcP1KO

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