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Gene Expression Data
Assay Details
Assay
Reference: J:283394 Hughes LJ, et al., Yap/Taz are required for establishing the cerebellar radial glia scaffold and proper foliation. Dev Biol. 2020 Jan 1;457(1):150-162
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7435351
Gene symbol: Crb3
Gene name: crumbs family member 3
Antibody: anti-pan-Crb
Detection system: Secondary antibody coupled to Cy3
Assay notes: The specificity of the antibody used was not detailed; all family members have been annotated.
Results
Specimen 8G: embryonic day 16.5 (more )
Note: Double labeled: red - Crbs; green - Ctnnb1 (assay MGI:7434581).
Structure Cell Type Level Pattern Image Note
TS24: cerebellum ventricular layer neuroepithelial stem cell Present Regionally restricted 8G Expressed in the apical junctional complex of neuroepithelium at the ventricular surface continuously.
Specimen 8H: embryonic day 16.5; Tg(Nes-cre)1Atp/0, Wwtr1tm1.1Eno/Wwtr1tm1.1Eno, Yap1tm1.1Eno/Yap1tm1.1Eno (more )
Note: Conditional mutant. Double labeled: red - Crbs; green - Ctnnb1 (assay MGI:7434581).
Structure Cell Type Level Pattern Image Note
TS24: cerebellum ventricular layer neuroepithelial stem cell Absent 8H
Specimen 8K: embryonic day 14.5 (more )
Note: Double labeled: red - Crbs; green - Yap1 (assay MGI:7434448).
Structure Cell Type Level Pattern Image Note
TS22: cerebellum ventricular layer neuroepithelial stem cell Present Regionally restricted 8K Expressed in the apical junctional complex of neuroepithelium at the ventricular surface continuously.
Specimen 8L: embryonic day 14.5; Tg(GFAP-cre)25Mes/0, Wwtr1tm1.1Eno/Wwtr1tm1.1Eno, Yap1tm1.1Eno/Yap1tm1.1Eno (more )
Note: Conditional mutant. Double labeled: red - Crbs; green - Yap1 (assay MGI:7434448).
Structure Cell Type Level Pattern Image Note
TS22: cerebellum ventricular layer neuroepithelial stem cell Present Regionally restricted 8L Loss of apical localization of Crbs correlated with the Yap1-reduced area.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
MGI 6.23 		The Jackson Laboratory