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Gene Expression Data
Assay Details
Assay
Reference: J:157660 Dowdy CR, et al., Definitive hematopoiesis requires Runx1 C-terminal-mediated subnuclear targeting and transactivation. Hum Mol Genet. 2010 Mar 15;19(6):1048-57
Assay type: RT-PCR
MGI Accession ID: MGI:7432784
Gene symbol: Spi1
Gene name: Spi-1 proto-oncogene
Probe: PU.1-pK, PU.1-pL
Assay notes: Quantitative RT-PCR. Relative expression was normalized to Gapdh.
Results Image: 5A PU.1
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
+/+ E12.5 TS20: embryo Present 1 µg; total RNA involves: 129S4/SvJae * C57BL/6 Not Specified
+/Runx1Q307X E12.5 TS20: embryo Present 1 µg; total RNA involves: 129S4/SvJae * C57BL/6 Runx1tm1.1Gss/Runx1+Not Specified
Runx1Q307X/Runx1Q307X E12.5 TS20: embryo Present (a) 1 µg; total RNA involves: 129S4/SvJae * C57BL/6 Runx1tm1.1Gss/Runx1tm1.1GssNot Specified
Notes:
(a) Expression in this mutant has decreased compared to wild-type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
MGI 6.23 		The Jackson Laboratory