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Gene Expression Data
Assay Details
Assay
Reference: J:147207 Wu M, et al., Zfp521 antagonizes Runx2, delays osteoblast differentiation in vitro, and promotes bone formation in vivo. Bone. 2009 Apr;44(4):528-36
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7398546
Gene symbol: Zfp521
Gene name: zinc finger protein 521
Antibody: anti-Zfp521
Assay notes: Two antibodies were described, but the one used in this assay was not detailed. Anti-Zfp521 MGI:7398543 may have been used in this assay.
Results
Specimen 1G (top): postnatal newborn (more )
Structure Cell Type Level Pattern Image Note
TS27: long bone epiphyseal plate proliferative zone chondrocyte Weak Regionally restricted 1G Merge (top) , 1G ZFP (top) Low expression in resting and proliferating chondrocytes.
TS27: long bone epiphyseal plate resting zone chondrocyte Weak Regionally restricted 1G Merge (top) , 1G ZFP (top) Low expression in resting and proliferating chondrocytes.
TS27: long bone epiphyseal plate growth plate cartilage chondrocyte Strong Regionally restricted 1G ZFP (top) , 1G Merge (top) Markedly higher expression in prehypertrophic growth plate cartilage chondrocytes.
TS27: metatarsus growth plate cartilage chondrocyte Strong Regionally restricted 1G ZFP (top) , 1G Merge (top) Markedly higher expression in prehypertrophic growth plate cartilage chondrocytes.
TS27: long bone epiphyseal plate hypertrophic zone hypertrophic chondrocyte Weak 1G ZFP (top) , 1G Merge (top) Low expression in hypertrophic chondrocyte.
Specimen 1G (center): postnatal month 6 (more )
Structure Cell Type Level Pattern Image Note
TS28: humerus osteoblast Strong Regionally restricted 1G Merge (center) , 1G ZFP (center) Strong expression in periosteum and endosteum, restricted to osteoblasts and 1 or 2 layers of pre-osteoblast cells.
TS28: humerus preosteoblast Strong Regionally restricted 1G Merge (center) , 1G ZFP (center) Strong expression in periosteum and endosteum, restricted to osteoblasts and 1 or 2 layers of pre-osteoblast cells.
TS28: periosteum Strong Regionally restricted 1G Merge (center) , 1G ZFP (center)
Specimen 1G (lower): postnatal month 6 (more )
Structure Cell Type Level Pattern Image Note
TS28: humerus osteocyte Present 1G Merge (lower) , 1G ZFP (lower)
TS28: humerus osteoclast Absent
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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