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Gene Expression Data
Assay Details
Assay
Reference: J:329966 Bell CJ, et al., Borcs6 is required for endo-lysosomal degradation during early development. Mol Reprod Dev. 2022 Aug;89(8):337-350
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7398485
Gene symbol: Rab7
Gene name: RAB7, member RAS oncogene family
Antibody: anti-Rab7 [D95F2] (9367)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 4a/c'/c''': embryonic day 6.5 (more )
Note: Triple labeled: red - Rab7; green - E-cadherin (Cdh1 assay MGI:7398481); white - Lamp1 (assay MGI:7398486).
Structure Level Pattern Image Note
TS9: primitive endoderm Weak Widespread 4a , 4c' , 4c''' Expression in lysosomal membrane. Faint expression throughout all cells.
Specimen 4b/d'/d''': embryonic day 6.5; Borcs6tm1.1(KOMP)Vlcg/Borcs6tm1.1(KOMP)Vlcg (more )
Note: Triple labeled: red - Rab7; green - E-cadherin (Cdh1 assay MGI:7398481); white - Lamp1 (assay MGI:7398486).
Structure Level Pattern Image Note
TS9: primitive endoderm Present Regionally restricted 4b , 4d''' , 4d' Expression in large, discrete vesicles primarily in visceral endoderm.
Specimen 4e/e''/g: embryonic day 6.5 (more )
Note: Double labeled: red - Rab7; white - Lamp1 (assay MGI:7398486).
Structure Level Pattern Image Note
TS9: primitive endoderm Present Regionally restricted 4e , 4e'' , 4g Higher levels of expression in proximal half of visceral endoderm thatn in distal half.
Specimen 4f/f''/h: embryonic day 6.5; Borcs6tm1.1(KOMP)Vlcg/Borcs6+ (more )
Note: Double labeled: red - Rab7; white - Lamp1 (assay MGI:7398486).
Structure Level Pattern Image Note
TS9: primitive endoderm Present Regionally restricted 4h , 4f'' , 4f Lysosomes disprersed evenly throughout visceral endoderm. No difference between proximal and distal parts. Large discrete patches non-overlapping with Lamp1 expression.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory