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Gene Expression Data
Assay Details
Assay
Reference: J:327232 Wu JPH, et al., The Transcription Factor Pou3f1 Sheds Light on the Development and Molecular Diversity of Glutamatergic Cerebellar Nuclear Neurons in the Mouse. Front Mol Neurosci. 2022;15:921901
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7336060
Gene symbol: Pou3f1
Gene name: POU domain, class 3, transcription factor 1
Antibody: anti-OCT6 (clone KT110) (MABN738)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 2D: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: cerebellum Present Regionally restricted 2D Between E12.5 and E15.5 expression in cells in nuclear transitory zone and nowhere else in developing cerebellum.

Specimen 2E: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: cerebellum white matter Present Regionally restricted 2E Expression in clusters of cells in cerebellar white matter in the forming nuclear region.

Specimen 4E,E': embryonic day 15.5 (more )
Structure Cell Type Level Pattern Image Note
TS23: cerebellum cerebellum glutamatergic neuron Present Regionally restricted 4E' , 4E Expression in nuclear transitory zone. Expression in glutamatergic cerebellar nuclear (CN) neurons.

Specimen 4F,F': embryonic day 15.5; Atoh1tm2Hzo/Atoh1tm2Hzo (more )
Structure Level Pattern Image Note
TS23: cerebellum Trace Single cells 4F , 4F' Expression completely absent in nuclear transitory zone. Residual Pou3f1 expression attributable to very few residual Atoh1-expressing cells.

Specimen 5B,B': embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: cerebellum white matter Present 5B , 5B' Expression in cells residing in cerebellar white matter.

Specimen 5C,C': embryonic day 18.5; Pax6Sey-Neu/Pax6Sey-Neu (more )
Structure Level Pattern Image Note
TS26: cerebellum white matter Present 5C' , 5C Expression persists in Sey mutant.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
04/23/2024
MGI 6.23
The Jackson Laboratory