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Gene Expression Data
Assay Details
Assay
Reference: J:252045 Cubillos-Rojas M, et al., The HERC2 ubiquitin ligase is essential for embryonic development and regulates motor coordination. Oncotarget. 2016 Aug 30;7(35):56083-56106
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7335056
Gene symbol: Herc2
Gene name: HECT and RLD domain containing E3 ubiquitin protein ligase 2
Antibody: anti-HERC2
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 9A: postnatal week 9 (more )
Note: Double labeled: green - Herc2; red - Calb1 (assay MGI:7335055).
Structure Cell Type Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Purkinje cell Present 9A right , 9A left Colocalized with Calb1 in a continuous cell layer.
Specimen 9B: postnatal week 9 (more )
Note: Double labeled: green - Herc2; red - Calb1 (assay MGI:7335055).
Structure Cell Type Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Purkinje cell Present 9B right , 9B left Colocalized with Calb1 in a continuous cell layer.
Specimen 9C: postnatal week 9; Herc2Gt(AR0530)Wtsi/Herc2+ (more )
Note: Double labeled: green - Herc2; red - Calb1 (assay MGI:7335055).
Structure Cell Type Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Purkinje cell Present Regionally restricted 9C right , 9C left Parasagittal bands of Purkinje cells loss in the vermis and paravermal zones and areas of extensive Purkinje cell loss in the cerebellar paraflocculus.
Specimen 9D: postnatal week 9; Herc2Gt(AR0530)Wtsi/Herc2+ (more )
Note: Double labeled: green - Herc2; red - Calb1 (assay MGI:7335055).
Structure Cell Type Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Purkinje cell Present Regionally restricted 9D left , 9D right Parasagittal bands of Purkinje cells loss in the vermis and paravermal zones.
Specimen 9E: postnatal week 9; Herc2Gt(AR0530)Wtsi/Herc2+ (more )
Note: Double labeled: green - Herc2; red - Calb1 (assay MGI:7335055).
Structure Cell Type Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Purkinje cell Present Regionally restricted 9E right , 9E left Extensive Purkinje cell loss in the cerebellar paraflocculus.
Specimen 12A: postnatal week 9 (more )
Note: Double labeled: green - Herc2; red - Sqstm1 (assay MGI:7335057).
Structure Cell Type Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Purkinje cell Present 12A left , 12A right Colocalized with Sqstm1/p62 in cell somata.
Specimen 12B: postnatal week 9; Herc2Gt(AR0530)Wtsi/Herc2+ (more )
Note: Double labeled: green - Herc2; red - Sqstm1 (assay MGI:7335057).
Structure Cell Type Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Purkinje cell Present 12B left , 12B right Colocalized with Sqstm1/p62 in dendrites, axonal torpedoes and cell somata. Absence of Purkinje cell bodies were seen.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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