About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:252045 Cubillos-Rojas M, et al., The HERC2 ubiquitin ligase is essential for embryonic development and regulates motor coordination. Oncotarget. 2016 Aug 30;7(35):56083-56106
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7335056
Gene symbol: Herc2
Gene name: HECT and RLD domain containing E3 ubiquitin protein ligase 2
Antibody: anti-HERC2
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 9A: postnatal week 9 (more )
Note: Double labeled: green - Herc2; red - Calb1 (assay MGI:7335055).
Structure Cell Type Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Purkinje cell Present 9A right , 9A left Colocalized with Calb1 in a continuous cell layer.
Specimen 9B: postnatal week 9 (more )
Note: Double labeled: green - Herc2; red - Calb1 (assay MGI:7335055).
Structure Cell Type Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Purkinje cell Present 9B right , 9B left Colocalized with Calb1 in a continuous cell layer.
Specimen 9C: postnatal week 9; Herc2Gt(AR0530)Wtsi/Herc2+ (more )
Note: Double labeled: green - Herc2; red - Calb1 (assay MGI:7335055).
Structure Cell Type Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Purkinje cell Present Regionally restricted 9C right , 9C left Parasagittal bands of Purkinje cells loss in the vermis and paravermal zones and areas of extensive Purkinje cell loss in the cerebellar paraflocculus.
Specimen 9D: postnatal week 9; Herc2Gt(AR0530)Wtsi/Herc2+ (more )
Note: Double labeled: green - Herc2; red - Calb1 (assay MGI:7335055).
Structure Cell Type Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Purkinje cell Present Regionally restricted 9D left , 9D right Parasagittal bands of Purkinje cells loss in the vermis and paravermal zones.
Specimen 9E: postnatal week 9; Herc2Gt(AR0530)Wtsi/Herc2+ (more )
Note: Double labeled: green - Herc2; red - Calb1 (assay MGI:7335055).
Structure Cell Type Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Purkinje cell Present Regionally restricted 9E right , 9E left Extensive Purkinje cell loss in the cerebellar paraflocculus.
Specimen 12A: postnatal week 9 (more )
Note: Double labeled: green - Herc2; red - Sqstm1 (assay MGI:7335057).
Structure Cell Type Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Purkinje cell Present 12A left , 12A right Colocalized with Sqstm1/p62 in cell somata.
Specimen 12B: postnatal week 9; Herc2Gt(AR0530)Wtsi/Herc2+ (more )
Note: Double labeled: green - Herc2; red - Sqstm1 (assay MGI:7335057).
Structure Cell Type Level Pattern Image Note
TS28: cerebellum Purkinje cell layer Purkinje cell Present 12B left , 12B right Colocalized with Sqstm1/p62 in dendrites, axonal torpedoes and cell somata. Absence of Purkinje cell bodies were seen.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
04/23/2024
MGI 6.23 		The Jackson Laboratory