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Gene Expression Data
Assay Details
Assay
Reference: J:85207 Shirahama-Noda K, et al., Biosynthetic processing of cathepsins and lysosomal degradation are abolished in asparaginyl endopeptidase-deficient mice. J Biol Chem. 2003 Aug 29;278(35):33194-9
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7334881
Gene symbol: Lgmn
Gene name: legumain
Antibody: anti-AEP
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 4A: postnatal adult (more )
Structure Level Pattern Image Note
TS28: renal cortex Present Regionally restricted 4A (right) Expression in restricted region of cortex.
TS28: renal proximal tubule Present Punctate 4A (right) Prominent expression in proximal tubules. Expression localized to punctate structures in apical region within cell.
TS28: renal distal tubule Weak 4A (right)
TS28: glomerular tuft Absent 4A (right)
Specimen not shown: postnatal adult (more )
Structure Level Pattern Image Note
TS28: renal medulla Absent
TS28: collecting duct Absent No expression in collecting or urinary tubules.
Specimen 4B: postnatal adult (more )
Note: Double labeled: green - AEP (Lgmn); green - Ctsd (assay MGI:7334882).
Structure Level Pattern Image Note
TS28: renal proximal tubule Present Regionally restricted 4B (middle) , 4B (left) Expression mostly in apical side of cells.
Specimen 4C: postnatal adult (more )
Note: Double labeled: green - AEP (Lgmn); green - Lamp2 (assay MGI:7334883).
Structure Level Pattern Image Note
TS28: renal proximal tubule Present Regionally restricted 4C (left) , 4C (middle) Co-expression with Lamp2 in late endosomes.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory