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Gene Expression Data
Assay Details
Assay
Reference: J:321237 Edwards NA, et al., P66Shc, a key regulator of metabolism and mitochondrial ROS production, is dysregulated by mouse embryo culture. Mol Hum Reprod. 2016 Sep;22(9):634-47
Assay type: RT-PCR
MGI Accession ID: MGI:7328568
Gene symbol: Shc1
Gene name: src homology 2 domain-containing transforming protein C1
Probe: p66Shc-pA, p66Shc-pB
Probe preparation: labelled with SYBR green
Assay notes: Quantitative RT-PCR. Relative expression was normalized to Ppia and H2afz.
Results Image: 1A
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
Zygote E0.5 (a) TS1: 1-cell stage conceptus Present Not Specified; total RNA CD-1 Not Specified
2-cell E1.0 (a) TS2: 2-cell stage conceptus Present Not Specified; total RNA CD-1 Not Specified
8-cell E2.5 (a) TS3: 8-cell stage conceptus Present Not Specified; total RNA CD-1 Not Specified
Blastocyst E3.5 (a) TS5: blastocyst-stage conceptus Present (b) Not Specified; total RNA CD-1 Not Specified
Notes:
(a) Age assigned by curator based on morphological criteria supplied by authors.
(b) Relative mRNA abundance increases from eight cell to blastocyst-stage embryos.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
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