Mtnr1b Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:256805 Tao J, et al., Circadian Rhythm Regulates Development of Enamel in Mouse Mandibular First Molar. PLoS One. 2016;11(8):e0159946
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7327338
Gene symbol:	Mtnr1b
Gene name:	melatonin receptor 1B

Antibody:	MEL-1A/B-R Antibody (H-120): sc-30017
Detection system:	Secondary antibody coupled to horseradish peroxidase
Assay notes:	The specificity of the antibody used was not detailed; both family members have been annotated.

Results

2A-E19.5 ©

2B-E19.5 ©

Specimen 2A/B E19.5: embryonic day 19.5 (more

)

Structure	Level	Image
TS26: inner enamel epithelium	Weak	2A-E19.5 , 2B-E19.5
TS26: molar dental papilla	Weak	2A-E19.5 , 2B-E19.5
TS26: stellate reticulum	Weak	2A-E19.5 , 2B-E19.5
TS26: stratum intermedium	Weak	2A-E19.5 , 2B-E19.5
TS26: mandible	Weak	2A-E19.5

2A-D0 ©

2B-D0 ©

Specimen 2A/B D0: postnatal day 0 (more

)

Structure	Level	Image
TS27: inner enamel epithelium	Weak	2A-D0 , 2B-D0
TS27: molar dental papilla	Weak	2A-D0 , 2B-D0
TS27: stellate reticulum	Weak	2A-D0 , 2B-D0
TS27: stratum intermedium	Weak	2A-D0 , 2B-D0
TS27: alveolar process	Weak	2A-D0
TS27: mandible	Weak	2A-D0

2A-D1 ©

2B-D1 ©

Specimen 2A/B D1: postnatal day 1 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS27: molar dental papilla	odontoblast	Trace		2B-D1 , 2A-D1	Expression was barely detectable.
TS27: enamel epithelium		Trace		2B-D1 , 2A-D1	Expression was barely detectable.

2A-D2 ©

2B-D2 ©

Specimen 2A/B D2: postnatal day 2 (more

)

Structure	Cell Type	Level	Image
TS27: enamel epithelium	ameloblast	Strong	2B-D2 , 2A-D2
TS27: molar dental papilla	odontoblast	Present	2A-D2 , 2B-D2
TS27: alveolar process		Present	2A-D2
TS27: mandible		Present	2A-D2

2A-D3 ©

2B-D3 ©

Specimen 2A/B D3: postnatal day 3 (more

)

Structure	Cell Type	Level	Image
TS27: enamel epithelium	ameloblast	Strong	2B-D3 , 2A-D3
TS27: molar dental papilla	odontoblast	Strong	2B-D3 , 2A-D3
TS27: alveolar process		Strong	2A-D3
TS27: mandible		Strong	2A-D3

2A-D4 ©

2B-D4 ©

Specimen 2A/B D4: postnatal day 4 (more

)

Structure	Cell Type	Level	Image
TS28: enamel epithelium	ameloblast	Strong	2B-D4 , 2A-D4
TS28: molar dental papilla	odontoblast	Strong	2A-D4 , 2B-D4
TS28: alveolar process		Strong	2A-D4
TS28: mandible		Strong	2A-D4

2A-D5 ©

2B-D5 ©

Specimen 2A/B D5: postnatal day 5 (more

)

Structure	Cell Type	Level	Image	Note
TS28: enamel epithelium	ameloblast	Present	2B-D5 , 2A-D5	Reduced expression compared with that of P2-P4.
TS28: alveolar process		Present	2A-D5	Reduced expression compared with that of P2-P4.
TS28: mandible		Present	2A-D5	Reduced expression compared with that of P2-P4.
TS28: molar dental papilla		Present	2A-D5	Reduced expression compared with that of P2-P4.

2A-D6 ©

2B-D6 ©

Specimen 2A/B D6: postnatal day 6 (more

)

Structure	Cell Type	Level	Image	Note
TS28: enamel epithelium	ameloblast	Present	2A-D6 , 2B-D6	Reduced expression compared with that of P2-P4.
TS28: alveolar process		Present	2A-D6	Reduced expression compared with that of P2-P4.
TS28: mandible		Present	2A-D6	Reduced expression compared with that of P2-P4.
TS28: molar dental papilla		Present	2A-D6	Reduced expression compared with that of P2-P4.

5A-D3 ©

5B-D3 ©

Specimen 5A/B D3: postnatal day 3 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS27: enamel epithelium	ameloblast	Strong		5A-D3 , 5B-D3
TS27: molar dental papilla	odontoblast	Strong		5A-D3 , 5B-D3

5A-D4 ©

5B-D4 ©

Specimen 5A/B D4: postnatal day 4 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: enamel epithelium	ameloblast	Strong		5A-D4 , 5B-D4
TS28: molar dental papilla	odontoblast	Strong		5B-D4 , 5A-D4

8A MTs ©

8B MTs ©

Specimen 8A/B D0: postnatal day 0 (more

)

Structure	Cell Type	Level	Image
TS27: alveolar process		Present	8A MTs
TS27: mandible		Present	8A MTs
TS27: inner enamel epithelium		Present	8B MTs , 8A MTs
TS27: stratum intermedium		Present	8B MTs , 8A MTs
TS27: molar dental papilla	odontoblast	Present	8B MTs , 8A MTs

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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