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Gene Expression Data
Assay Details
Assay
Reference: J:324823 Mitchel K, et al., Hoxa5 Activity Across the Lateral Somitic Frontier Regulates Development of the Mouse Sternum. Front Cell Dev Biol. 2022;10:806545
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7309976
Gene symbol: Hoxa5
Gene name: homeobox A5
Antibody: Hoxa5 antibody
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 1A: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: somite Present Regionally restricted 1A Expressed broadly in somites, with the exception of prospective skeletal muscle.
TS19: future arm mesenchyme Present Regionally restricted 1A Expressed in the mesenchyme of the proximal forelimb.
TS19: trunk mesenchyme Present Regionally restricted 1A Expressed in the axillary region, where the sternal progenitors were found.
TS19: body wall mesenchyme Present Single cells 1A Few positive cells in ventral body wall mesenchyme.
Specimen 5N: embryonic day 14.5 (more )
Note: interclavicular mesenchyme
Structure Level Pattern Image Note
TS22: sternum Weak Regionally restricted 5N' , 5N Expressed in the paired ventral condensations.
Specimen 5O: embryonic day 14.5 (more )
Note: interclavicular mesenchyme
Structure Level Pattern Image Note
TS22: sternum Weak Regionally restricted 5O , 5O' Expressed in the paired ventral condensations.
Specimen 5P: embryonic day 14.5 (more )
Note: intercostoclavicular mesenchyme
Structure Level Pattern Image Note
TS22: sternum Present Regionally restricted 5P , 5P' Increased expression within the intercostoclavicular mesenchyme of the ventral sternum.
TS22: trunk mesenchyme Present Regionally restricted 5P , 5P' Expressed in a domain lateral to but continuous with the developing sternum.
Specimen 5Q: embryonic day 14.5 (more )
Note: intercostoclavicular mesenchyme
Structure Level Pattern Image Note
TS22: sternum Present Regionally restricted 5Q , 5Q' Increased expression within the intercostoclavicular mesenchyme of the ventral sternum.
TS22: trunk mesenchyme Present Regionally restricted 5Q' , 5Q Expressed in a domain lateral to but continuous with the developing sternum.
Specimen 5R: embryonic day 14.5 (more )
Note: intercostoclavicular mesenchyme
Structure Level Pattern Image Note
TS22: sternum Present Regionally restricted 5R , 5R' Increased expression within the intercostoclavicular mesenchyme at the lateral edges of the ventral sternum.
TS22: trunk mesenchyme Present Regionally restricted 5R , 5R' Expressed in a domain lateral to but continuous with the developing sternum.
Specimen 5S: embryonic day 14.5 (more )
Note: intercostoclavicular mesenchyme
Structure Level Pattern Image Note
TS22: sternum Present Regionally restricted 5S' , 5S Increased expression within the intercostoclavicular mesenchyme of the ventral sternum.
TS22: trunk mesenchyme Present Regionally restricted 5S' , 5S Expressed in a domain lateral to but continuous with the developing sternum.
Specimen 5T: embryonic day 14.5 (more )
Note: Rib 1
Structure Level Pattern Image Note
TS22: sternum Present Regionally restricted 5T' , 5T Expressed in the ventral presternum.
TS22: T1 rib cartilage condensation Present 5T , 5T'
TS22: trunk mesenchyme Present Regionally restricted 5T , 5T' Expressed in a domain lateral to but continuous with the developing sternum.
Specimen 5U: embryonic day 14.5 (more )
Note: Rib 1
Structure Level Pattern Image Note
TS22: sternum Present Regionally restricted 5U , 5U' Expressed in the ventral presternum.
TS22: trunk mesenchyme Present Regionally restricted 5U' , 5U Expressed in a domain lateral to but continuous with the developing sternum.
Specimen 5V: embryonic day 14.5 (more )
Note: Rib 1
Structure Level Pattern Image Note
TS22: trunk mesenchyme Present Regionally restricted 5V' , 5V Expressed in a dorsal domain surrounding infrahyoid muscles. Expressed in a domain lateral to but continuous with the developing sternum.
TS22: sternum Present Regionally restricted 5V , 5V' Expressed in the ventral presternum.
Specimen 5W: embryonic day 14.5 (more )
Note: Rib 1
Structure Level Pattern Image Note
TS22: trunk mesenchyme Present Regionally restricted 5W , 5W' Expressed in a dorsal domain surrounding infrahyoid muscles and a domain lateral to but continuous with the developing sternum.
TS22: sternum Present Regionally restricted 5W , 5W' Expressed in the ventral presternum.
TS22: perichondrium Present 5W' , 5W
Specimen 5X: embryonic day 14.5 (more )
Note: Intercostal
Structure Level Pattern Image Note
TS22: trunk connective tissue Present Regionally restricted 5X , 5X' Expressed in a dorsal domain surrounding infrahyoid muscles, likely marking the connective tissue.
TS22: sternum Present Regionally restricted 5X' , 5X No expression in sternum posterior to the T1 site.
Specimen 5Y: embryonic day 14.5 (more )
Note: Rib 2
Structure Level Pattern Image Note
TS22: trunk connective tissue Present Regionally restricted 5Y , 5Y' Expressed in a dorsal domain surrounding infrahyoid muscles, likely marking the connective tissue.
TS22: perichondrium Present 5Y' , 5Y
TS22: sternum Present Regionally restricted 5Y' , 5Y No expression in sternum posterior to the T1 site.
Specimen 5Z: embryonic day 14.5 (more )
Note: Rib 3
Structure Level Pattern Image Note
TS22: mesothelium Present Regionally restricted 5Z , 5Z' Expressed in the body wall mesothelium.
TS22: perichondrium Present 5Z' , 5Z
TS22: sternum Present Regionally restricted 5Z , 5Z' No expression in sternum posterior to the T1 site.
Specimen 6A/J: embryonic day 13.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Meox1tm1(cre)Jpa/Meox1+ (more )
Note: Conditional mutant. Interclavicular mesenchyme. Triple labeled: green - Hoxa5; blue - Sox9 (assay MGI:7309991); red - Cre reporter.
Structure Level Pattern Image Note
TS21: pectoral-thoracic pre-cartilage condensation Present Regionally restricted 6J , 6A" , 6A , 6A' Co-expressed with Sox9 only in the paired ventral condensations of the presternum. None of these presternum elements showed contribution from Meox1-Cre labelled somite derived cells.
Specimen 6B/K: embryonic day 13.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Meox1tm1(cre)Jpa/Meox1+ (more )
Note: Conditional mutant. Interclavicular mesenchyme. Triple labeled: green - Hoxa5; blue - Sox9 (assay MGI:7309991); red - Cre reporter.
Structure Level Pattern Image Note
TS21: pectoral-thoracic pre-cartilage condensation Present Regionally restricted 6B , 6B" , 6K , 6B' Co-expressed with Sox9 only in the paired ventral condensations of the presternum. None of these presternum elements showed contribution from Meox1-Cre labelled somite derived cells.
Specimen 6C/L: embryonic day 13.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Meox1tm1(cre)Jpa/Meox1+ (more )
Note: Conditional mutant. Intercostoclavicular mesenchyme. Triple labeled: green - Hoxa5; blue - Sox9 (assay MGI:7309991); red - Cre reporter.
Structure Level Pattern Image Note
TS21: pectoral-thoracic pre-cartilage condensation Present Regionally restricted 6C" , 6L , 6C' , 6C Co-expressed with Sox9 within the intercostoclavicular mesenchyme at the lateral edges of the ventral presternum, with the Hoxa5+ only domain extending more broadly ventral to Sox9. None of these presternum elements showed contribution from Meox1-Cre labelled somite derived cells.
Specimen 6D/M: embryonic day 13.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Meox1tm1(cre)Jpa/Meox1+ (more )
Note: Conditional mutant. Intercostoclavicular mesenchyme. Triple labeled: green - Hoxa5; blue - Sox9 (assay MGI:7309991); red - Cre reporter.
Structure Level Pattern Image Note
TS21: pectoral-thoracic pre-cartilage condensation Present Regionally restricted 6M , 6D" , 6D , 6D' Co-expressed with Sox9 within the intercostoclavicular mesenchyme at the lateral edges of the ventral presternum, with the Hoxa5+ only domain extending more broadly ventral to Sox9. None of these presternum elements showed contribution from Meox1-Cre labelled somite derived cells.
Specimen 6E/N: embryonic day 13.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Meox1tm1(cre)Jpa/Meox1+ (more )
Note: Conditional mutant. Intercostoclavicular mesenchyme. Triple labeled: green - Hoxa5; blue - Sox9 (assay MGI:7309991); red - Cre reporter.
Structure Level Pattern Image Note
TS21: pectoral-thoracic pre-cartilage condensation Present Regionally restricted 6E" , 6N , 6E' , 6E Co-expressed with Sox9 within the intercostoclavicular mesenchyme at the lateral edges of the ventral presternum, with the Hoxa5 domain extending more broadly ventral to Sox9. None of these presternum elements showed contribution from Meox1-Cre labelled somite derived cells.
Specimen 6F/O: embryonic day 13.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Meox1tm1(cre)Jpa/Meox1+ (more )
Note: Conditional mutant. Rib1. Triple labeled: green - Hoxa5; blue - Sox9 (assay MGI:7309991); red - Cre reporter.
Structure Level Pattern Image Note
TS21: T1 rib cartilage condensation Present 6O , 6F" , 6F' , 6F Co-expressed with Sox9, spanning the site of rib 1-to-presternum fusion.
TS21: perichondrium Present 6F , 6O , 6F" , 6F'
TS21: pectoral-thoracic pre-cartilage condensation Present Regionally restricted 6F" , 6O , 6F , 6F' Co-expressed with Sox9 in the presternum. Rib 1 appeared to make contact with the rounded, medial region. Sternal bar fusion was also incomplete. None of these presternum elements showed contribution from Meox1-Cre labelled somite derived cells.
Specimen 6G/P: embryonic day 13.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Meox1tm1(cre)Jpa/Meox1+ (more )
Note: Conditional mutant. Rib1. Triple labeled: green - Hoxa5; blue - Sox9 (assay MGI:7309991); red - Cre reporter.
Structure Level Pattern Image Note
TS21: T1 rib cartilage condensation Present 6G" , 6P , 6G , 6G' Co-expressed with Sox9, spanning the site of rib 1-to-presternum fusion.
TS21: perichondrium Present 6G" , 6G' , 6P , 6G
TS21: pectoral-thoracic pre-cartilage condensation Present Regionally restricted 6P , 6G' , 6G , 6G" Co-expressed with Sox9 in the presternum. Rib 1 appeared to make contact with the rounded, medial region. Sternal bar fusion was also incomplete. None of these presternum elements showed contribution from Meox1-Cre labelled somite derived cells.
Specimen 6H/Q: embryonic day 13.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Meox1tm1(cre)Jpa/Meox1+ (more )
Note: Conditional mutant. Rib1. Triple labeled: green - Hoxa5; blue - Sox9 (assay MGI:7309991); red - Cre reporter.
Structure Level Pattern Image Note
TS21: T1 rib cartilage condensation Present 6Q , 6H' , 6H" , 6H Co-expressed with Sox9, spanning the site of rib 1-to-presternum fusion.
TS21: perichondrium Present 6H , 6H' , 6H" , 6Q
TS21: pectoral-thoracic pre-cartilage condensation Present Regionally restricted 6H , 6H' , 6Q , 6H" Co-expressed with Sox9 in the presternum. Rib 1 appeared to make contact with the rounded, medial region. Sternal bar fusion was also incomplete. None of these presternum elements showed contribution from Meox1-Cre labelled somite derived cells.
Specimen 6I/R: embryonic day 13.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Meox1tm1(cre)Jpa/Meox1+ (more )
Note: Conditional mutant. Rib2/3.Triple labeled: green - Hoxa5; blue - Sox9 (assay MGI:7309991); red - Cre reporter.
Structure Level Pattern Image Note
TS21: perichondrium Present 6I' , 6I , 6I" , 6R
TS21: T2 rib cartilage condensation Present 6I" , 6R , 6I , 6I' Co-expressed with Sox9.
TS21: trunk connective tissue Present Regionally restricted 6I , 6I' , 6I" , 6R Expressed in the connective tissue of the infrahyoid muscles, dorsal to the sternum.
Specimen 7I: embryonic day 12.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Meox1tm1(cre)Jpa/Meox1+ (more )
Note: Conditional mutant. Double labeled: green - Hoxa5; red - Cre reporter.
Structure Level Pattern Image Note
TS20: trunk mesenchyme Present Regionally restricted 7I" , 7I Expressed in a domain laterally adjacent to but not overlapping the Sox9 positive mesenchyme.
Specimen 7J: embryonic day 12.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Meox1tm1(cre)Jpa/Meox1+ (more )
Note: Conditional mutant. Interclavicular mesenchyme. Double labeled: green - Hoxa5; red - Cre reporter.
Structure Level Pattern Image Note
TS20: trunk mesenchyme Present Regionally restricted 7J" , 7J Expressed in a domain (Meox1-Cre lineage/RFP-negative) laterally adjacent to but not overlapping the Sox9 positive interclavicular mesenchyme.
Specimen 7K: embryonic day 12.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Meox1tm1(cre)Jpa/Meox1+ (more )
Note: Conditional mutant. Interclavicular mesenchyme. Double labeled: green - Hoxa5; red - Cre reporter.
Structure Level Pattern Image Note
TS20: trunk mesenchyme Present Regionally restricted 7K" , 7K Expressed in a domain (Meox1-Cre lineage/RFP-negative) laterally adjacent to but not overlapping the Sox9 positive interclavicular mesenchyme.
Specimen 7L: embryonic day 12.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Meox1tm1(cre)Jpa/Meox1+ (more )
Note: Conditional mutant. Intercostoclavicular mesenchyme. Double labeled: green - Hoxa5; red - Cre reporter.
Structure Level Pattern Image Note
TS20: pectoral-thoracic pre-cartilage condensation Strong Regionally restricted 7L , 7L" Expressed in the ventral elements, but remained excluded from the dorsal elements.
Specimen 7M: embryonic day 12.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Meox1tm1(cre)Jpa/Meox1+ (more )
Note: Conditional mutant. Intercostoclavicular mesenchyme. Double labeled: green - Hoxa5; red - Cre reporter.
Structure Level Pattern Image Note
TS20: pectoral-thoracic pre-cartilage condensation Present Regionally restricted 7M" , 7M Expressed in the ventral elements, but remained excluded from the dorsal elements. expression is excluded from the most medial cells of the ventral elements, but overlapped with Sox9 in the lateral part of these condensations.
TS20: trunk mesenchyme Present Regionally restricted 7M , 7M" Expressed in a lateral plate mesoderm-derived lateral domain that was Sox9 negative, although appear continuous with the Sox9 positive domain.
Specimen 7N: embryonic day 12.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Meox1tm1(cre)Jpa/Meox1+ (more )
Note: Conditional mutant. Rib 1. Double labeled: green - Hoxa5; red - Cre reporter.
Structure Level Pattern Image Note
TS20: pectoral-thoracic pre-cartilage condensation Present Regionally restricted 7N , 7N" Expressed in the ventral elements, but remained excluded from the dorsal elements. expression is excluded from the most medial cells of the ventral elements, but overlapped with Sox9 in the lateral part of these condensations.
TS20: T1 rib pre-cartilage condensation Present 7N" , 7N Meox1-Cre lineage/RFP-positive.
TS20: trunk mesenchyme Present Regionally restricted 7N" , 7N Expressed in a lateral domain that was Sox9 negative and throughout the region of rib 1 attachment, although appear continuous with the Sox9 positive domain.
Specimen 7O: embryonic day 12.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Meox1tm1(cre)Jpa/Meox1+ (more )
Note: Conditional mutant. Rib 1. Double labeled: green - Hoxa5; red - Cre reporter.
Structure Level Pattern Image Note
TS20: pectoral-thoracic pre-cartilage condensation Present Regionally restricted 7O , 7O" Expressed in the ventral elements, but remained excluded from the dorsal elements. expression is excluded from the most medial cells of the ventral elements, but overlapped with Sox9 in the lateral part of these condensations.
TS20: T1 rib pre-cartilage condensation Present 7O , 7O" Meox1-Cre lineage/RFP-positive.
TS20: trunk mesenchyme Present Regionally restricted 7O" , 7O Expressed in a lateral domain that was Sox9 negative and throughout the region of rib 1 attachment, although appear continuous with the Sox9 positive domain.
Specimen 7P: embryonic day 12.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Meox1tm1(cre)Jpa/Meox1+ (more )
Note: Conditional mutant. Rib 2. Double labeled: green - Hoxa5; red - Cre reporter.
Structure Level Pattern Image Note
TS20: trunk connective tissue Present Regionally restricted 7P" , 7P Expressed in a dorsal domain surrounding infrahyoid muscles, likely marking the connective tissue.
TS20: T2 rib pre-cartilage condensation Present 7P" , 7P Meox1-Cre lineage/RFP-positive.
Specimen 8F: embryonic day 12.0; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant. Interclavicular mesenchyme. Double labeled: green - Hoxa5; red - Cre reporter.
Structure Level Pattern Image Note
TS20: trunk mesenchyme Present Regionally restricted 8F" , 8F Expressed lateral to Sox9 domain.
Specimen 8G: embryonic day 12.0; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant. Interclavicular mesenchyme. Double labeled: green - Hoxa5; red - Cre reporter.
Structure Level Pattern Image Note
TS20: trunk mesenchyme Present Regionally restricted 8G , 8G" Expressed lateral to Sox9 domain.
Specimen 8H: embryonic day 12.0; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant. Intercostoclavicular mesenchyme. Double labeled: green - Hoxa5; red - Cre reporter.
Structure Level Pattern Image Note
TS20: trunk mesenchyme Present Regionally restricted 8H , 8H" Expressed lateral to Sox9 domain.
TS20: pectoral-thoracic pre-cartilage condensation Present Regionally restricted 8H , 8H" Also expressed in the lateral-most edges of the SOX9 domain.
Specimen 8I: embryonic day 12.0; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant. Intercostoclavicular mesenchyme. Double labeled: green - Hoxa5; red - Cre reporter.
Structure Level Pattern Image Note
TS20: trunk mesenchyme Present Regionally restricted 8I , 8I" Expressed lateral to Sox9 domain.
TS20: pectoral-thoracic pre-cartilage condensation Present Regionally restricted 8I" , 8I Also expressed in the lateral-most edges of the SOX9 domain.
Specimen 8J: embryonic day 12.0; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant. Rib 1. Double labeled: green - Hoxa5; red - Cre reporter.
Structure Level Pattern Image Note
TS20: trunk mesenchyme Present Regionally restricted 8J" , 8J Expressed in a population of lateral plate mesoderm-derived cells (Prrx1-Cre lineage/RFP+) lining the medial edge of the incoming rib.
Specimen 8L/M: embryonic day 11.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant. Double labeled: green - Hoxa5; red - Cre reporter.
Structure Level Pattern Image Note
TS19: trunk mesenchyme Present Regionally restricted 8M , 8L Expressed in lateral plate mesoderm-derived domain (Prrx1-Cre lineage/RFP+) anterior to the forelimb.
Specimen 8N: embryonic day 11.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant. Double labeled: green - Hoxa5; blue - Ebf3 (assay MGI:7309998).
Structure Level Pattern Image Note
TS19: trunk mesenchyme Present Regionally restricted 8N Coexpressed with Ebf3 in a subdomain of Hoxa5-positive cells.
Specimen 8P/Q: embryonic day 11.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant. Double labeled: green - Hoxa5; red - Cre reporter.
Structure Level Pattern Image Note
TS19: trunk mesenchyme Present Regionally restricted 8P , 8Q Expressed ventral to the forelimb, a region previously shown to contain sternal bar progenitors.
Specimen 8R: embryonic day 11.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant. Double labeled: green - Hoxa5; blue - Ebf3 (assay MGI:7309998).
Structure Level Pattern Image Note
TS19: trunk mesenchyme Present Regionally restricted 8R Coexpressed with Ebf3 in a subdomain of Hoxa5-positive cells.
Specimen S2B: embryonic day 14.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant. Interclavicular mesenchyme. Double labeled: green - Hoxa5; red - Cre reporter.
Structure Level Pattern Image Note
TS22: sternum Weak S2B , S2B' , S2B"' Co-expressed with Prrx1 lineage cells (lateral plate mesoderm lineage) in the paired ventral condensations.
Specimen S2D: embryonic day 14.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant. Intercostoclavicular mesenchyme. Double labeled: green - Hoxa5; red - Cre reporter.
Structure Level Pattern Image Note
TS22: sternum Present Regionally restricted S2D , S2D"' , S2D' Co-expressed with Prrx1 lineage cells (lateral plate mesoderm lineage) within the intercostoclavicular mesenchyme of the ventral sternum.
Specimen S2F: embryonic day 14.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant. Intercostoclavicular mesenchyme. Double labeled: green - Hoxa5; red - Cre reporter.
Structure Level Pattern Image Note
TS22: sternum Present Regionally restricted S2F , S2F' , S2F"' Co-expressed with Prrx1 lineage cells (lateral plate mesoderm lineage) within the intercostoclavicular mesenchyme of the ventral sternum.
TS22: trunk mesenchyme Present Regionally restricted S2F' , S2F , S2F"' Co-expressed with Prrx1 lineage cells (lateral plate mesoderm lineage) in a domain lateral to but continuous with the developing sternum.
Specimen S2H: embryonic day 14.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant. Rib 1. Double labeled: green - Hoxa5; red - Cre reporter.
Structure Level Pattern Image Note
TS22: sternum Present Regionally restricted S2H' , S2H , S2H"' Co-expressed with Prrx1 lineage cells (lateral plate mesoderm lineage) in the ventral presternum.
TS22: T1 rib cartilage condensation Present S2H' , S2H"' , S2H Not co-expressed with Prrx1 lineage cells (lateral plate mesoderm lineage).
Specimen S2J: embryonic day 14.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant. Rib 1. Double labeled: green - Hoxa5; red - Cre reporter.
Structure Level Pattern Image Note
TS22: sternum Present Regionally restricted S2J' , S2J , S2J"' Co-expressed with Prrx1 lineage cells (lateral plate mesoderm lineage) in the ventral presternum.
TS22: T1 rib cartilage condensation Present S2J , S2J' , S2J"' Not co-expressed with Prrx1 lineage cells (lateral plate mesoderm lineage).
Specimen S2L: embryonic day 14.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant. Rib 2. Double labeled: green - Hoxa5; red - Cre reporter.
Structure Level Pattern Image Note
TS22: trunk connective tissue Present Regionally restricted S2L' , S2L"' , S2L Co-expressed with Prrx1 lineage cells (lateral plate mesoderm lineage) in a dorsal domain surrounding infrahyoid muscles, likely marking the connective tissue.
TS22: perichondrium Present S2L"' , S2L , S2L'
Specimen S3A/G: embryonic day 14.5 (more )
Note: Interclavicular mesenchyme. Double labeled: green - Hoxa5; red - Sox9 (assay MGI:7309996).
Structure Level Pattern Image Note
TS22: sternum Weak Regionally restricted S3A , S3A' , S3G Co-expressed with Sox9 only in the paired ventral condensations.
Specimen S3B/H: embryonic day 14.5 (more )
Note: Interclavicular mesenchyme. Double labeled: green - Hoxa5; red - Sox9 (assay MGI:7309996).
Structure Level Pattern Image Note
TS22: sternum Weak Regionally restricted S3H , S3B , S3B' Co-expressed with Sox9 only in the paired ventral condensations.
Specimen S3C/I: embryonic day 14.5 (more )
Note: Intercostoclavicular mesenchyme. Double labeled: green - Hoxa5; red - Sox9 (assay MGI:7309996).
Structure Level Pattern Image Note
TS22: sternum Present Regionally restricted S3I , S3C' , S3C Co-expressed with Sox9 within the intercostoclavicular mesenchyme at the lateral edges of the ventral sternum, with the Hoxa5 domain extending more broadly ventral to Sox9.
TS22: trunk mesenchyme Present Regionally restricted S3I , S3C , S3C' Expressed in a domain lateral to but continuous with the developing sternum.
Specimen S3D/J: embryonic day 14.5 (more )
Note: Intercostoclavicular mesenchyme. Double labeled: green - Hoxa5; red - Sox9 (assay MGI:7309996).
Structure Level Pattern Image Note
TS22: sternum Present Regionally restricted S3D , S3D' , S3J Co-expressed with Sox9 within the intercostoclavicular mesenchyme of the ventral sternum, with the Hoxa5 domain extending more broadly ventral to Sox9.
TS22: trunk mesenchyme Present Regionally restricted S3D' , S3D , S3J Expressed in a domain lateral to but continuous with the developing sternum.
Specimen S3E/K: embryonic day 14.5 (more )
Note: Rib 1. Double labeled: green - Hoxa5; red - Sox9 (assay MGI:7309996).
Structure Level Pattern Image Note
TS22: sternum Present Regionally restricted S3E , S3E' , S3K Expressed in the ventral presternum.
TS22: T1 rib cartilage condensation Present S3K , S3E' , S3E Co-expressed with Sox9, spanning the site of rib 1-to-sternum fusion.
TS22: perichondrium Present S3E , S3K , S3E'
TS22: trunk connective tissue Present Regionally restricted S3E , S3K , S3E' Expressed in the connective tissue of the infrahyoid muscles, dorsal to the sternum.
Specimen S3F/L: embryonic day 14.5 (more )
Note: Rib 2. Double labeled: green - Hoxa5; red - Sox9 (assay MGI:7309996).
Structure Level Pattern Image Note
TS22: sternum Present Regionally restricted S3F , S3F' , S3L No expression in sternum posterior to the T1 site.
TS22: trunk connective tissue Present Regionally restricted S3F' , S3F , S3L Expressed in a dorsal domain surrounding infrahyoid muscles, likely marking the connective tissue.
TS22: T2 rib cartilage condensation Present S3F , S3F' , S3L Co-expressed with Sox9.
TS22: perichondrium Present S3F , S3L , S3F'
Specimen S4L: embryonic day 13.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant. Interclavicular mesenchyme. Double labeled: green - Hoxa5; red - Cre reporter.
Structure Level Pattern Image Note
TS21: pectoral-thoracic pre-cartilage condensation Weak Regionally restricted S4L , S4L' , S4L"' Largely absent from the presternum.
Specimen S4N: embryonic day 13.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant. Intercostoclavicular mesenchyme. Double labeled: green - Hoxa5; red - Cre reporter.
Structure Level Pattern Image Note
TS21: pectoral-thoracic pre-cartilage condensation Present Regionally restricted S4N"' , S4N , S4N' Expressed in the intercostoclavicular mesenchyme of the ventral presternum.
TS21: trunk mesenchyme Present Regionally restricted S4N , S4N"' , S4N' Co-expressed with Prrx1 lineage in a domain lateral to but continuous with the developing sternum.
Specimen S4P: embryonic day 13.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant. Rib 1. Double labeled: green - Hoxa5; red - Cre reporter.
Structure Level Pattern Image Note
TS21: pectoral-thoracic pre-cartilage condensation Present Regionally restricted S4P' , S4P , S4P"' Expressed in the intercostoclavicular mesenchyme of the ventral presternum anterior to T1. Rib1 made contact with the Hoxa5-expressing ventral-lateral presternum.
TS21: T1 rib cartilage condensation Present S4P' , S4P , S4P"'
Specimen S4R: embryonic day 13.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant. Rib 1. Double labeled: green - Hoxa5; red - Cre reporter.
Structure Level Pattern Image Note
TS21: pectoral-thoracic pre-cartilage condensation Present Regionally restricted S4R , S4R"' , S4R' Rib 1 contact with the Hoxa5+ unfused sternal bars. Expression was also present dorsally surrounding the point of rib 1 contact.
TS21: trunk mesenchyme Present Regionally restricted S4R"' , S4R , S4R' Co-expressed with Prrx1 lineage in a domain lateral to but continuous with the developing sternum.
TS21: T1 rib cartilage condensation Present S4R' , S4R"' , S4R
Specimen S4T: embryonic day 13.5; Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant. Rib 1/2. Double labeled: green - Hoxa5; red - Cre reporter.
Structure Level Pattern Image Note
TS21: T1 rib cartilage condensation Present S4T , S4T"' , S4T'
TS21: T2 rib cartilage condensation Present S4T , S4T"' , S4T'
TS21: pectoral-thoracic pre-cartilage condensation Present Regionally restricted S4T' , S4T"' , S4T Co-expressed with Prrx1 lineage cells (lateral plate mesoderm lineage) in the ventral presternum. Sternal Hoxa5 marked the points of rib contact.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory