Cyp4a12a RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:308925 Chikada H, et al., Establishment and analysis of a mouse model that regulates sex-related differences in liver drug metabolism. Lab Invest. 2018 Nov;98(11):1500-1511
Assay type:	RT-PCR
MGI Accession ID:	MGI:7263220
Gene symbol:	Cyp4a12a
Gene name:	cytochrome P450, family 4, subfamily a, polypeptide 12a

Probe:	Cyp4a12a/12b-pA, Cyp4a12a/12b-pB
Assay notes:	Quantitative RT-PCR. Relative expression was normalized to Hprt.Expression of male WT mouse livers was set to 1.0.

Results

Image: 2_Cyp4a12a/12b

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
MWT	P w 8-11	TS28: liver	Strong (a)	Not Specified; total RNA	involves: C57BL/6		Male	Wild-type specimens were cre-negative, exact background was not detailed.
FWT	P w 8-11	TS28: liver	Present	Not Specified; total RNA	involves: C57BL/6		Female	Wild-type specimens were cre-negative, exact background was not detailed.
MLKO	P w 8-11	TS28: liver	Trace (b)	Not Specified; total RNA	involves: C57BL/6 * DBA	Bcl6^tm1.1Mtto/Bcl6^tm1.1Mtto, Speer6-ps1^{Tg(Alb-cre)21Mgn}/0	Male	Conditional mutant.
FLKO	P w 8-11	TS28: liver	Trace (b)	Not Specified; total RNA	involves: C57BL/6 * DBA	Bcl6^tm1.1Mtto/Bcl6^tm1.1Mtto, Speer6-ps1^{Tg(Alb-cre)21Mgn}/0	Female	Conditional mutant.

Notes:
(a) Expression in male livers was significantly higher than in female livers.
(b) Expression was significantly downregulated compared to wild-type. Expression between male and female mutant mice was similar.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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