Gene Expression Data
Assay Details
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Assay
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Reference:
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J:308929
De Vincenti AP, et al., Lrig1 and Lrig3 cooperate to control Ret receptor signaling, sensory axonal growth and epidermal innervation. Development. 2021 Aug 15;148(16):dev197020
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7258077
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Gene symbol:
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Gfra2
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Gene name:
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glial cell line derived neurotrophic factor family receptor alpha 2
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Antibody:
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Anti-GFR alpha2 (AF429)
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Detection system:
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Secondary antibody coupled to Cy3
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Assay notes:
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Zamboni's fixative was used. A mixture of antibodies was used to detect both Gfra1 and Gfra2. Both genes are annotated (Gfra1 is shown in assay MGI:7258073).
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Results
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Specimen
4A WT:
postnatal adult
(more  )
Specimen
4A WT:
(close  )
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Genetic Background:
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involves: 129
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: foot skin |
Present |
Regionally restricted |
4A GFRa1/2 left
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Expression is in nonpeptidergic neuron fibers in the glabrous hind paw skin.
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Specimen
4A Lrig1WT/Lrig3KO:
(close )
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Genetic Background:
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involves: 129
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Mutant Allele(s):
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Lrig3tm1.1Hhed/Lrig3tm1.1Hhed
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
4A Lrig1HT/Lrig3KO:
(close )
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Genetic Background:
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involves: 129
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Mutant Allele(s):
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Lrig3tm1.1Hhed/Lrig3tm1.1Hhed
Lrig1tm1.1Hhed/Lrig1+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: foot skin |
Present |
Regionally restricted |
4A GFRa1/2 right
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The density of positive nonpeptidergic neuron fibers in the glabrous hind paw skin increased (37% per unit length) in this mutant compared with wild-type and Lrig1WT/Lrig3KO mice.
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Analysis Tools