Des Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:273624 Bourgeois JR, et al., Loss of the neurodevelopmental Joubert syndrome causing protein, Ahi1, causes motor and muscle development delays independent of central nervous system involvement. Dev Biol. 2019 Apr 1;448(1):36-47
Assay type:	Western blot
MGI Accession ID:	MGI:7255025
Gene symbol:	Des
Gene name:	desmin

Antibody:	anti-Desmin (D3)
Detection system:	Secondary antibody coupled to horseradish peroxidase

Results

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	53 kDa	46 kDa	Genetic Background	Mutant Allele(s)	Sex
+/+	E18.5	TS26: hindlimb	Present	Trace	involves: C57BL/6J * FVB/NJ		Not Specified
+/+	E18.5	TS26: hindlimb	Present	Trace	involves: C57BL/6J * FVB/NJ		Not Specified
+/+	E18.5	TS26: hindlimb	Present	Trace	involves: C57BL/6J * FVB/NJ		Not Specified
-/-	E18.5	TS26: hindlimb	Weak (a)	Trace (b)	involves: C57BL/6J * FVB/NJ	Ahi1^tm1Rujf/Ahi1^tm1Rujf	Not Specified
-/-	E18.5	TS26: hindlimb	Weak (a)	Trace (b)	involves: C57BL/6J * FVB/NJ	Ahi1^tm1Rujf/Ahi1^tm1Rujf	Not Specified
-/-	E18.5	TS26: hindlimb	Weak (a)	Trace (b)	involves: C57BL/6J * FVB/NJ	Ahi1^tm1Rujf/Ahi1^tm1Rujf	Not Specified

Notes:
(a) Reduced levels of 53 kDa compared with control.
(b) Authors report increased level of degradation products relative to total expression levels, as well as reduction in total expression levels.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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