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Gene Expression Data
Assay Details
Assay
Reference: J:320610 Auguste D, et al., Disease-causing mutations of RhoGDIalpha induce Rac1 hyperactivation in podocytes. Small GTPases. 2016 Apr 2;7(2):107-21
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6888180
Gene symbol: Rac1
Gene name: Rac family small GTPase 1
Antibody: anti-active Rac1-GTP (#26903)
Detection system: Secondary antibody coupled to Alexa Fluor 555
Results
Specimen 10B E13.5: embryonic day 13.5 (more )
Note: Triple labeled: red - active Rac1; green - Wt1 (assay MGI:6888179); blue - DAPI.
Structure Cell Type Level Pattern Image Note
TS21: comma-shaped body metanephric podocyte Present Regionally restricted 10B E13.5 Active Rac1 , 10B E13.5 Merge Expression clearly detect at plasma membrane in podocyte precursors.
Specimen 10B E16.5: embryonic day 16.5 (more )
Note: Triple labeled: red - active Rac1; green - Wt1 (assay MGI:6888179); blue - DAPI.
Structure Level Pattern Image Note
TS24: capillary loop renal corpuscle Weak Regionally restricted 10B E16.5 Active Rac1 , 10B E16.5 Merge Less expression of active Rac1 at E16.5 than at E13.5. Expression limited to center of glomerulus.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory