Dbh RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:228903 Irie M, et al., Cognitive Function Related to the Sirh11/Zcchc16 Gene Acquired from an LTR Retrotransposon in Eutherians. PLoS Genet. 2015 Sep;11(9):e1005521
Assay type:	RT-PCR
MGI Accession ID:	MGI:6887472
Gene symbol:	Dbh
Gene name:	dopamine beta hydroxylase

Probe:	Dbh-F1, Dbh-R1
Probe preparation:	labelled with SYBR green
Assay notes:	Quantitative RT-PCR. Relative expression was normalized to Actb.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
Mesencephalon WT	P	TS28: midbrain	Present	1 µg; total RNA	C57BL/6J		Not Specified
Mesencephalon KO	P	TS28: midbrain	Present (a)	1 µg; total RNA	B6.Cg-Rtl4^tm1.1Fuis	Rtl4^tm1.1Fuis/Rtl4^tm1.1Fuis	Not Specified
Brainstem WT	P	TS28: brainstem	Present (b)	1 µg; total RNA	C57BL/6J		Not Specified
Brainstem KO	P	TS28: brainstem	Present (a)	1 µg; total RNA	B6.Cg-Rtl4^tm1.1Fuis	Rtl4^tm1.1Fuis/Rtl4^tm1.1Fuis	Not Specified

Notes:
(a) There was no significant difference in expression level of the mutant compared to wild type.
(b) The brainstem exhibits an approximately 32 fold greater expression level than the cerebrum (graphed in Fig. 4B left).

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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