Gene Expression Data
Assay Details
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Assay
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Reference:
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J:315091
Roques M, et al., Endoglin expression level discriminates long-term hematopoietic from short-term clonogenic progenitor cells in the aorta. Haematologica. 2012 Jul;97(7):975-9
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6867057
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Gene symbol:
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Eng
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Gene name:
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endoglin
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Results
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Specimen
1A:
embryonic day 9.5
(more  )
Note: Triple labeled: green - CD105 (Eng); red - CD31 (Pecam1 assay MGI:6867058); blue - DAPI.
Specimen
1A:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 9.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - CD105 (Eng); red - CD31 (Pecam1 assay MGI:6867058); blue - DAPI.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS15: cardiovascular system endothelium |
blood vessel endothelial cell |
Present |
Regionally restricted |
1A
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Expression in all vascular endothelial cells (ECs), co-expressed with CD31 (Pecam1).
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Specimen
1B:
embryonic day 9.5
(more )
Note: Triple labeled: green - CD105 (Eng); red - CD144 (Cdh5 assay MGI:6867059); blue - DAPI.
Specimen
1B:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 9.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - CD105 (Eng); red - CD144 (Cdh5 assay MGI:6867059); blue - DAPI.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS15: cardiovascular system endothelium |
blood vessel endothelial cell |
Present |
Regionally restricted |
1B
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Expression in all vascular endothelial cells (ECs), co-expressed with CD105 (Cdh5).
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Specimen
1C:
embryonic day 10.5
(more )
Note: Triple labeled: green - CD105 (Eng); red - CD31 (Pecam1 assay MGI:6867058); blue - DAPI.
Specimen
1C:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 10.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - CD105 (Eng); red - CD31 (Pecam1 assay MGI:6867058); blue - DAPI.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS17: blood vessel endothelium |
blood vessel endothelial cell |
Present |
Regionally restricted |
1C
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Expression in all vascular endothelial cells (ECs), co-expressed with CD31 (Pecam1). Co-expression similar to that seen at E9.
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Specimen
1D:
embryonic day 10.5
(more )
Note: Triple labeled: green - CD105 (Eng); red - CD144 (Cdh5 assay MGI:6867059); blue - DAPI.
Specimen
1D:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 10.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - CD105 (Eng); red - CD144 (Cdh5 assay MGI:6867059); blue - DAPI.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS17: blood vessel endothelium |
blood vessel endothelial cell |
Present |
Regionally restricted |
1D
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Expression in all vascular endothelial cells (ECs), co-expressed with CD105 (Cdh5). Co-expression similar to that seen at E9.
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Specimen
1E:
embryonic day 10.5
(more )
Note: Triple labeled: green - CD105 (Eng); red - c-kit (Kit assay MGI:6867060); blue - DAPI.
Specimen
1E:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 10.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Triple labeled: green - CD105 (Eng); red - c-kit (Kit assay MGI:6867060); blue - DAPI.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS17: blood vessel endothelium |
aortic endothelial cell |
Present |
Regionally restricted |
1E
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| TS17: dorsal aorta |
AGM hematopoietic stem cell |
Present |
Regionally restricted |
1E
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Co-expression with c-kit (Kit) at the level of hematopoietic clusters.
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Specimen
1F:
embryonic day 11.5
(more )
Specimen
1F:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 11.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS19: blood vessel endothelium |
blood vessel endothelial cell |
Present |
Regionally restricted |
1F
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Expression in most if not all vessels. Expression restricted to endothelium.
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| TS19: liver |
fetal liver hematopoietic progenitor cell |
Present |
Regionally restricted |
1F
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Expression in hematopoietic stem cells (HSCs) of fetal liver.
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| TS19: aorta-gonad-mesonephros |
AGM hematopoietic stem cell |
Present |
Regionally restricted |
1F
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Expression in hematopoietic stem cells (HSCs) of the aorta-gonad-mesonephros (AGM) region. Expression in hematopoietic clusterx in aortic floor
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Specimen
1G:
embryonic day 11.5
(more )
Specimen
1G:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 11.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS19: dorsal aorta |
AGM hematopoietic stem cell |
Present |
Regionally restricted |
1G
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Variable levels of expression in hematopoietic cluster-composing cells.
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Analysis Tools