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Gene Expression Data
Assay Details
Assay
Reference: J:237701 Saare M, et al., Expression Pattern and Localization Dynamics of Guanine Nucleotide Exchange Factor RIC8 during Mouse Oogenesis. PLoS One. 2015;10(6):e0129131
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6864141
Gene symbol: Gpsm2
Gene name: G-protein signalling modulator 2 (AGS3-like, C. elegans)
Antibody: anti-LGN
Detection system: Alexa Fluor
Results
Specimen 3H/I: postnatal adult (more )
Note: germinal vesicle stage. Triple labeled: red - LGN (Gpsm2); green - RIC8 (Ric8a assay MGI:6864135); blue - DAPI.
Structure Level Pattern Image Note
TS28: primary oocyte Weak Regionally restricted 3H , 3I Expression weakly detectable in cytoplasm.
Specimen 3h/i: postnatal adult (more )
Note: Triple labeled: red - LGN (Gpsm2); green - RIC8 (Ric8a assay MGI:6864135); blue - DAPI.
Structure Level Pattern Image Note
TS28: primary oocyte Ambiguous 3h , 3i No co-localization with RIC8 (Ric8a).
Specimen 4B/C: postnatal adult (more )
Note: metaphase of meiosis I. Triple labeled: red - LGN (Gpsm2); green - RIC8 (Ric8a assay MGI:6864135); blue - DAPI.
Structure Level Pattern Image Note
TS28: oocyte Present Regionally restricted 4B , 4C High extent of co-localization with RIC8 (Ric8a) at meiotic spindle apparatus.
Specimen 4E/F: postnatal adult (more )
Note: anaphase/telophase of meiosis I. Triple labeled: red - LGN (Gpsm2); green - RIC8 (Ric8a assay MGI:6864135); blue - DAPI.
Structure Level Pattern Image Note
TS28: oocyte Present Regionally restricted 4E , 4F Expression associated with meiotic spindle during anaphase and telophase. Expression localized more to chromosomes.
Specimen 4H/I: postnatal adult (more )
Note: metaphase of meiosis II. Triple labeled: red - LGN (Gpsm2); green - RIC8 (Ric8a assay MGI:6864135); blue - DAPI.
Structure Level Pattern Image Note
TS28: secondary oocyte Present Regionally restricted 4I , 4H Extent of colocalization with RIC8 (Ric8a) similar to MI oocytes.
Specimen 6E/F: embryonic day 0.5 (more )
Note: pronuclear stage. Triple labeled: red - LGN (Gpsm2); green - RIC8 (Ric8a assay MGI:6864135); blue - DAPI.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present Regionally restricted 6F , 6E Expression colocalized with RIC8 (Ric8a) in middle of spindle, but not at plus or minus ends
TS1: second polar body Present 6F , 6E Expression discernable in remains of second polar body .
Specimen 6e/f: embryonic day 0.5 (more )
Note: Triple labeled: red - LGN (Gpsm2); green - RIC8 (Ric8a assay MGI:6864135); blue - DAPI.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Ambiguous 6e , 6f Expression not detectable in pronuclei.
Specimen not shown: embryonic day 0.5 (more )
Note: metaphase I of first mitosis.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present Regionally restricted Co-localized with RIC8 (Ric8a) and Galphai1/2 (Gnai1, Gnai2) in mitotic spndle and cell cortex.
Specimen 7H/I: embryonic day 1.0 (more )
Note: Triple labeled: red - LGN (Gpsm2); green - RIC8 (Ric8a assay MGI:6864135); blue - DAPI.
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present Regionally restricted 7H , 7I In cell cortex, typcially co-localized with RIC8 (Ric8a) and Galphai1/2 (Gnai1, Gnai2). No expression in region between blastomeres.
Specimen 7h/i: embryonic day 1.0 (more )
Note: Triple labeled: red - LGN (Gpsm2); green - RIC8 (Ric8a assay MGI:6864135); blue - DAPI.
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present Regionally restricted 7i , 7h In apical cortex region of blastomeres, extensive ovarlap with RIC8 (Ric8a).
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory