Antibody:	anti-Coro1B
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS17: embryo	Present	Widespread	2A	Expressed at a range of expression levels.

Structure	Level	Pattern	Image	Note
TS19: brain	Present		2B
TS19: branchial arch	Present		2B
TS19: heart	Present		2B
TS19: neural tube	Present		2B
TS19: septum transversum	Present		2B

Structure	Level	Pattern	Image	Note
TS20: brain	Strong		2C
TS20: endocardial cushion	Strong		2C
TS20: spinal cord	Strong		2C

Structure	Level	Pattern	Image	Note
TS23: central nervous system	Present		2D	Expressed at a relative lower expression level compared with other tissues/organs.
TS23: gut epithelium	Present		2D
TS23: limb	Present		2D
TS23: liver	Present		2D
TS23: lower jaw	Present		2D
TS23: metanephros	Present		2D
TS23: nasal epithelium	Present		2D
TS23: thymus primordium	Present		2D
TS23: tongue	Present		2D

Structure	Level	Pattern	Image	Note
TS15: heart	Present		2E , 2F
TS15: atrioventricular canal cardiac muscle	Present		2E , 2F	Expressed at a higher level.

Structure	Level	Pattern	Image	Note
TS17: atrioventricular canal cardiac muscle	Present		2G
TS17: endocardial lining	Present		2G
TS17: endocardial tube	Present		2G
TS17: outflow tract cardiac muscle	Present		2G
TS17: visceral pericardium	Present		2G

Structure	Level	Pattern	Image	Note
TS19: atrioventricular endocardial cushion	Present		2H
TS19: endocardial lining	Present		2H
TS19: visceral pericardium	Present		2H

Structure	Level	Pattern	Image	Note
TS20: atrioventricular endocardial cushion	Strong		2L , 2K
TS20: endocardial lining	Strong		2L , 2K
TS20: visceral pericardium	Strong		2L , 2K
TS20: cardiac muscle tissue	Absent		2L , 2K

Structure	Level	Pattern	Image	Note
TS21: visceral pericardium	Present		7I

Structure	Level	Pattern	Image	Note
TS21: visceral pericardium	Present		7J	Modestly reduced expression compared with the wild-type.