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Gene Expression Data
Assay Details
Assay
Reference: J:150616 Gutknecht L, et al., Spatio-temporal expression of tryptophan hydroxylase isoforms in murine and human brain: convergent data from Tph2 knockout mice. Eur Neuropsychopharmacol. 2009 Apr;19(4):266-82
Assay type: RT-PCR
MGI Accession ID: MGI:6840948
Gene symbol: Tph1
Gene name: tryptophan hydroxylase 1
Probe: Tph1-pE, Tph1-pF
Probe preparation: labelled with SYBR green
Assay notes: Quantitative RT-PCR. Tissues were obtained from mice perfused with PBS to remove the majority blood components, or from mice without previous perfusion (control). No significant differences were found between perfused versus control tissues. Absolute copy numbers were normalized against two out of four most stable reference genes.
Results Image: 2_Tph1
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
perfused cortex P adult TS28: cerebral cortex Trace 0.5 µg; total RNA C57BL/6J Not Specified
control cortex P adult TS28: cerebral cortex Trace 0.5 µg; total RNA C57BL/6J Not Specified
perfused striatum P adult TS28: corpus striatum Trace 0.5 µg; total RNA C57BL/6J Not Specified
control striatum P adult TS28: corpus striatum Trace 0.5 µg; total RNA C57BL/6J Not Specified
perfused hippoc. P adult TS28: hippocampus Trace 0.5 µg; total RNA C57BL/6J Not Specified
control hippoc. P adult TS28: hippocampus Trace 0.5 µg; total RNA C57BL/6J Not Specified
perfused cereb. P adult TS28: cerebellum Trace 0.5 µg; total RNA C57BL/6J Not Specified
control cereb. P adult TS28: cerebellum Trace 0.5 µg; total RNA C57BL/6J Not Specified
perfused raphe P adult TS28: raphe nucleus Trace 0.5 µg; total RNA C57BL/6J Not Specified
control raphe P adult TS28: raphe nucleus Trace 0.5 µg; total RNA C57BL/6J Not Specified
perfused pin.gl. P adult TS28: pineal gland Very strong 0.5 µg; total RNA C57BL/6J Not Specified
control pin.gl. P adult TS28: pineal gland Very strong 0.5 µg; total RNA C57BL/6J Not Specified
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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