Gfi1b In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:314670 Thambyrajah R, et al., GFI1 proteins orchestrate the emergence of haematopoietic stem cells through recruitment of LSD1. Nat Cell Biol. 2016 Jan;18(1):21-32
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:6828607
Gene symbol:	Gfi1b
Gene name:	growth factor independent 1B

Reporter:	GFP
Antibody:	anti-GFP (NB100-1614)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

Specimen 4Ai: embryonic day 10.5; Gfi1^tm2Tmo/Gfi1⁺, Gfi1b^tm1Tmo/Gfi1b⁺ (more

)
Note: Multi-labeled: green - reporters for Gfi1b and Gfi1 (assay MGI:6828606); red - Pecam1 (assay MGI:6827463); yellow - Kit (assay MGI:6828601). Also stained with DAPI (blue).

Structure	Level	Pattern	Image	Note
TS17: hematopoietic system	Present	Clusters	4Ai GFP , 4Ai Merged	Expression is seen in intra-aortic haematopoietic clusters. (We know from other studies that the green seen in the endothelium is due to the other reporter.)

Specimen 4Aii: embryonic day 10.5; Gfi1^tm2Tmo/Gfi1^tm2Tmo, Gfi1b^tm1Tmo/Gfi1b⁺ (more

)
Note: Multi-labeled: green - reporters for Gfi1b and Gfi1 (assay MGI:6828606); red - Pecam1 (assay MGI:6827463); yellow - Kit (assay MGI:6828601). Also stained with DAPI (blue).

Structure	Level	Pattern	Image	Note
TS17: hematopoietic system	Present	Clusters	4Aii Merged , 4Aii GFP	Expression is seen in intra-aortic haematopoietic clusters. (We know from other studies that the green seen in the endothelium is due to the other reporter.)

Specimen 4Aiii: embryonic day 10.5; Gfi1^tm2Tmo/Gfi1⁺, Gfi1b^tm1Tmo/Gfi1b^tm1Tmo (more

)
Note: Multi-labeled: green - reporters for Gfi1b and Gfi1 (assay MGI:6828606); red - Pecam1 (assay MGI:6827463); yellow - Kit (assay MGI:6828601). Also stained with DAPI (blue).

Structure	Level	Pattern	Image	Note
TS17: hematopoietic system	Present	Clusters	4Aiii Merged , 4Aiii GFP	Expression is seen in intra-aortic haematopoietic clusters. (We know from other studies that the green seen in the endothelium is due to the other reporter.)

Specimen 4Aiv: embryonic day 10.5; Gfi1^tm2Tmo/Gfi1^tm2Tmo, Gfi1b^tm1Tmo/Gfi1b^tm1Tmo (more

)
Note: Multi-labeled: green - reporters for Gfi1b and Gfi1 (assay MGI:6828606); red - Pecam1 (assay MGI:6827463); yellow - Kit (assay MGI:6828601). Also stained with DAPI (blue).

Structure	Level	Pattern	Image	Note
TS17: hematopoietic system	Absent		4Aiv Merged , 4Aiv GFP	There are no expressing intra-aortic haematopoietic clusters. (We know from other studies that the green seen in the endothelium is due to the other reporter.)

Specimen S5Aii: embryonic day 10.5; Gfi1b^tm1Tmo/Gfi1b^tm1Tmo (more

)
Note: Triple labeled: green - Gfi1b reporter; red - Pecam1 (assay MGI:6827463); yellow - Kit (assay MGI:6828601). Also stained with DAPI (blue).

Structure	Level	Pattern	Image	Note
TS17: hematopoietic system	Present	Clusters	S5Aii Merge , S5Aii GFI1B	Expression is seen in intra-aortic haematopoietic clusters.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 05/07/2024 MGI 6.23