Gfi1b RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:314670 Thambyrajah R, et al., GFI1 proteins orchestrate the emergence of haematopoietic stem cells through recruitment of LSD1. Nat Cell Biol. 2016 Jan;18(1):21-32
Assay type:	RNA in situ
MGI Accession ID:	MGI:6827468
Gene symbol:	Gfi1b
Gene name:	growth factor independent 1B

Probe:	Gfi1b cDNA2
Probe preparation:	Antisense RNA

Results

Specimen S5b - het/het: embryonic day 10.5; Gfi1^tm2Tmo/Gfi1⁺, Gfi1b^tm1Tmo/Gfi1b⁺ (more

)
Note: Double labeled: red - Gfi1b RNA; brown - Pecam1 protein (assay MGI:6827467).

Structure	Level	Pattern	Image	Note
TS17: hematopoietic system	Present	Regionally restricted	S5B left	Expression is seen in intra-aortic haematopoietic clusters.
TS17: dorsal aorta	Absent		S5B left	No expression is seen in the endothelial lining of the dorsal aorta.

Specimen S5b - KO/het: embryonic day 10.5; Gfi1^tm2Tmo/Gfi1^tm2Tmo, Gfi1b^tm1Tmo/Gfi1b⁺ (more

)
Note: Double labeled: red - Gfi1b RNA; brown - Pecam1 protein (assay MGI:6827467).

Structure	Level	Pattern	Image	Note
TS17: hematopoietic system	Present	Regionally restricted	S5B center	Expression is seen in intra-aortic haematopoietic clusters.
TS17: dorsal aorta	Present	Regionally restricted	S5B right , S5B center	Expression is seen in the endothelial lining of the dorsal aorta.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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