Antibody:	20F8
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS24: embryo	Present		2A

Structure	Level	Pattern	Image	Note
TS24: embryo	Absent		2B

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Present		2C
TS24: cerebral cortex subventricular zone	Present		2C

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Absent		2D

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present	Regionally restricted	2E	Expression is in neurons and astrocytes and is absent in oligodendrocytes.

Structure	Level	Pattern	Image	Note
TS28: brain ependyma	Present		2F
TS28: choroid plexus	Present		2F

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present	Regionally restricted	2G	Expression is in neurons and astrocytes and is absent in oligodendrocytes.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	2H	Expression is in the larger motor neurons and Purkinje cells.

Structure	Level	Pattern	Image	Note
TS28: thymus cortex	Strong		3B
TS28: thymus medullary core	Present		3B
TS28: hassall's corpuscle	Absent		3B

Structure	Level	Pattern	Image	Note
TS28: alveolus epithelium	Present	Regionally restricted	3E	Expression is in the low columnar epithelial cells, Clara cells, and terminally differentiated ciliated cells.
TS28: respiratory bronchiole epithelium	Present	Regionally restricted	3E	Expression is in the low columnar epithelial cells, Clara cells, and terminally differentiated ciliated cells.
TS28: trachea epithelium	Present	Regionally restricted	3E	Expression is in the low columnar epithelial cells, Clara cells, and terminally differentiated ciliated cells.

Structure	Level	Pattern	Image	Note
TS28: islets of Langerhans	Very strong		3H
TS28: pancreatic duct	Weak	Regionally restricted	3H	Expression is in epithelial cells lining the pancreatic ducts.
TS28: pancreatic acinus	Absent		3H

Structure	Level	Pattern	Image	Note
TS28: juxtaglomerular complex	Strong		3K
TS28: distal convoluted tubule	Present		3K
TS28: renal proximal convoluted tubule	Present		3K
TS28: renal tubule	Present		3K
TS28: metanephros	Weak	Regionally restricted	3K	The majority of cells in the kidney had low expression.
TS28: glomerular tuft	Absent		3K

Structure	Level	Pattern	Image	Note
TS28: hair outer root sheath	Present		4B
TS28: sebaceous gland	Present		4B
TS28: hair bulb	Present	Regionally restricted	4B	Expression is in undifferentiated bulb cells.
TS28: epidermis basal layer	Present	Regionally restricted	4B	Expression is in squamous epithelial cells.

Structure	Level	Pattern	Image	Note
TS28: tongue epithelium	Present	Regionally restricted	4E	Expression is in the stratified squamous epithelial cells of the tongue, with the majority of expression confined to the basal layer of the germinativum.
TS28: serous gland	Present		4E
TS28: taste bud	Present		4E
TS28: dermis papillary layer	Absent		4E
TS28: filiform papillae	Absent		4E

Structure	Level	Pattern	Image	Note
TS28: endometrium epithelium	Strong		4H	Expression is cytoplasmic.
TS28: myometrium	Present		4H

Structure	Level	Pattern	Image	Note
TS28: mammary gland	Present		4K	Expression is in both the ductal and lobuloalveolar epithelial cells with lower levels in the myoepithelium and surrounding stroma.
TS28: mammary gland luminal epithelium	Present		4K
TS28: mammary gland duct	Present	Regionally restricted	4K	Expression is in epithelial cells.
TS28: mammary gland myoepithelium	Weak		4K

Structure	Level	Pattern	Image	Note
TS28: hair outer root sheath	Present		5B
TS28: sebaceous gland	Present		5B
TS28: epidermis basal layer	Present	Regionally restricted	5B	Expression is in squamous epithelial cells.
TS28: hair bulb	Present	Regionally restricted	5B	Expression is in undifferentiated bulb cells.

Structure	Level	Pattern	Image	Note
TS28: mammary gland	Very strong	Regionally restricted	5E	Expression is in the cap cells.
TS28: mammary duct terminal end bud	Present		5E

Structure	Level	Pattern	Image	Note
TS28: crypt of lieberkuhn	Strong		5H
TS28: small intestine smooth muscle	Present		5H

Structure	Level	Pattern	Image	Note
TS24: main olfactory bulb	Present	Regionally restricted		Expression is in the postmitotic and migrating neurons in the differentiating field.
TS24: spinal cord	Present	Regionally restricted		Expression is in the postmitotic and migrating neurons in the differentiating field.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Regionally restricted		Expression is in neurons and astrocytes.

Structure	Level	Pattern	Image	Note
TS28: endometrium	Strong
TS28: myometrium	Strong
TS28: mammary gland epithelium	Strong	Regionally restricted		Expression is in aveolar and ductal epithelium.
TS28: mammary duct terminal end bud	Strong	Regionally restricted		Strong in cap cells, weak in body cells.
TS28: corpus luteum	Weak
TS28: ovary follicle	Weak
TS28: mammary gland myoepithelium	Absent
TS28: surface epithelium of ovary	Absent

Structure	Cell Type	Level	Pattern	Image	Note
TS28: spermatocyte		Moderate
TS28: spermatogonium		Moderate
TS28: anterior prostate gland		Weak
TS28: dorsal prostatic epithelium		Weak
TS28: seminal vesicle		Weak
TS28: ventral prostatic epithelium		Weak
TS28: Leydig cell	Leydig cell	Absent
TS28: Sertoli cell	Sertoli cell	Absent

Structure	Level	Pattern	Image	Note
TS28: Brunner's gland	Strong
TS28: crypt of lieberkuhn	Strong
TS28: distal convoluted tubule	Strong
TS28: epidermis basal layer	Strong
TS28: islets of Langerhans	Strong
TS28: large intestine crypt of lieberkuhn	Strong
TS28: renal proximal convoluted tubule	Strong
TS28: sebaceous gland	Strong
TS28: brain	Strong	Regionally restricted		Expression is in neurons and astrocytes.
TS28: tongue epithelium	Strong	Regionally restricted		Expression is in the basal germinativum (squamous epithelium), absent in papilla.
TS28: bronchiole epithelium	Strong	Regionally restricted		Expression is in ciliated and Clara cells.
TS28: brain ependyma	Moderate
TS28: collecting duct	Moderate
TS28: colon muscularis	Moderate
TS28: hair bulb	Moderate
TS28: hair outer root sheath	Moderate
TS28: lymph node T cell domain	Moderate
TS28: small intestine smooth muscle	Moderate
TS28: spleen periarteriolar lymphatic sheath	Moderate
TS28: thymus cortex	Moderate	Regionally restricted		Expression is in cortical thymocytes.
TS28: salivary gland	Moderate	Regionally restricted		Expression is in tubuloalveolar ducts, absent in mucous cells and myoepithelial cells.
TS28: stomach	Moderate	Regionally restricted		Expression is in Chief cells, weak in parietal and foveolar cells, and is absent in mucous neck cells.
TS28: small intestine epithelium	Moderate	Regionally restricted		Expression is in crypst of Lieberkuhn, and Brunner's glands, absent in Goblet cells.
TS28: cerebellum Purkinje cell layer	Weak
TS28: pancreatic duct	Weak
TS28: thymus medullary core	Weak	Regionally restricted		Expression is in thymocytes.
TS28: cardiac muscle tissue	Absent
TS28: glomerular tuft	Absent
TS28: lymph node B cell domain	Absent
TS28: spleen primary B follicle	Absent
TS28: spleen secondary B follicle	Absent