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Gene Expression Data
Assay Details
Assay
Reference: J:265712 Dziedzic SA, et al., ABIN-1 regulates RIPK1 activation by linking Met1 ubiquitylation with Lys63 deubiquitylation in TNF-RSC. Nat Cell Biol. 2018 Jan;20(1):58-68
Assay type: Western blot
MGI Accession ID: MGI:6761334
Gene symbol: Mlkl
Gene name: mixed lineage kinase domain-like
Antibody: Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab196436)
Detection system: Secondary antibody coupled to horseradish peroxidase
Assay notes: Tissues were harvested in NP-40 lysis buffer, then treated with 6M urea to show the insoluble fraction, which is known to occur when cells undergo necroptosis. The antibody used recognizes the phosphorylated form of the protein.
Results Image: 7a lower p-MLKL
 Sample Information Bands Other Sample Information
Lane AgeStructure 50 kDa Genetic BackgroundMutant Allele(s)Sex
Head +/- 1 E16.5 TS24: head Ambiguous involves: C57BL/6J Tnip1tm1.1Ama/Tnip1+Not Specified
Head +/- 2 E16.5 TS24: head Ambiguous involves: C57BL/6J Tnip1tm1.1Ama/Tnip1+Not Specified
Head +/+ 1 E16.5 TS24: head Weak involves: C57BL/6J Not Specified
Head -/- 1 E16.5 TS24: head Present involves: C57BL/6J Tnip1tm1.1Ama/Tnip1tm1.1AmaNot Specified
Head +/+ 2 E16.5 TS24: head Weak involves: C57BL/6J Not Specified
Head -/- 2 E16.5 TS24: head Present involves: C57BL/6J Tnip1tm1.1Ama/Tnip1tm1.1AmaNot Specified
Head +/- 3 E16.5 TS24: head Weak involves: C57BL/6J Tnip1tm1.1Ama/Tnip1+Not Specified
Head +/- 4 E16.5 TS24: head Weak involves: C57BL/6J Tnip1tm1.1Ama/Tnip1+Not Specified
Body +/- 1 E16.5 TS24: limb
TS24: tail
TS24: trunk 		Weak involves: C57BL/6J Tnip1tm1.1Ama/Tnip1+Not Specified
Body +/- 2 E16.5 TS24: limb
TS24: tail
TS24: trunk 		Weak involves: C57BL/6J Tnip1tm1.1Ama/Tnip1+Not Specified
Body +/+ 1 E16.5 TS24: limb
TS24: tail
TS24: trunk 		Weak involves: C57BL/6J Not Specified
Body -/- 1 E16.5 TS24: limb
TS24: tail
TS24: trunk 		Present involves: C57BL/6J Tnip1tm1.1Ama/Tnip1tm1.1AmaNot Specified
Body +/+ 2 E16.5 TS24: limb
TS24: tail
TS24: trunk 		Weak involves: C57BL/6J Not Specified
Body -/- 2 E16.5 TS24: limb
TS24: tail
TS24: trunk 		Present involves: C57BL/6J Tnip1tm1.1Ama/Tnip1tm1.1AmaNot Specified
Body +/- 3 E16.5 TS24: limb
TS24: tail
TS24: trunk 		Weak involves: C57BL/6J Tnip1tm1.1Ama/Tnip1+Not Specified
Body +/- 4 E16.5 TS24: limb
TS24: tail
TS24: trunk 		Weak involves: C57BL/6J Tnip1tm1.1Ama/Tnip1+Not Specified
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
04/23/2024
MGI 6.23 		The Jackson Laboratory