Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:243896
Chevalier M, et al., The embryonic development of hindbrain respiratory networks is unaffected by mutation of the planar polarity protein Scribble. Neuroscience. 2017 Aug 15;357:160-171
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:6755482
|
|
Gene symbol:
|
Tacr1
|
|
Gene name:
|
tachykinin receptor 1
|
|
Antibody:
|
anti-NK1R
|
|
Detection system:
|
Secondary antibody coupled to Alexa Fluor 568
|
|
|
Results
|
Specimen
3B:
embryonic day 16.5
(more  )
Note: Double labeled: red - Tacr1; green - Isl1 (assay MGI:6755205 and Isl2 (MGI:6755557).
Specimen
3B:
(close  )
|
Genetic Background:
|
involves: C57BL/6J * NMRI
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: red - Tacr1; green - Isl1 (assay MGI:6755205 and Isl2 (MGI:6755557).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: medulla oblongata |
Present |
Regionally restricted |
3B right
,
3B left
|
Expression is in the embryonic parafacial respiratory group and the pre-botzinger complex.
|
Specimen
3C:
(close )
|
Genetic Background:
|
involves: C57BL/6J * NMRI
|
|
Mutant Allele(s):
|
ScribCrc/Scrib+
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: red - Tacr1; green - Isl1 (assay MGI:6755205 and Isl2 (MGI:6755557).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: medulla oblongata |
Present |
|
3C left
,
3C right
|
Expression is in the embryonic parafacial respiratory group and the pre-botzinger complex.
|
Specimen
3D:
(close )
|
Genetic Background:
|
involves: C57BL/6J * NMRI
|
|
Mutant Allele(s):
|
ScribCrc/ScribCrc
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: red - Tacr1; green - Isl1 (assay MGI:6755205 and Isl2 (MGI:6755557).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: brainstem nucleus |
Present |
Regionally restricted |
3D right
,
3D left
|
Expression is in a group of cells at the pial surface of the hindbrain, but not in direct proximity to the facial nucleus and is abnormally located in a slightly more dorsal and medial position. The rostro-caudal and medio-lateral extensions of this group of cells in the mutant were restricted compared to wild-type.
|
| TS24: medulla oblongata |
Present |
Regionally restricted |
3D right
,
3D left
|
Expression is in a group of cells at the pial surface of the hindbrain, but not in direct proximity to the facial nucleus and is abnormally located in a slightly more dorsal and medial position. The rostro-caudal and medio-lateral extensions of this group of cells in the mutant were restricted compared to wild-type.
|
Specimen
5B:
embryonic day 16.5
(more )
Specimen
5B:
(close )
|
Genetic Background:
|
involves: C57BL/6J * NMRI
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: brainstem nucleus |
Present |
Regionally restricted |
5B
|
Expression is in the pre-botzinger complex.
|
| TS24: medulla oblongata |
Present |
Regionally restricted |
5B
|
Expression is in the pre-botzinger complex.
|
Specimen
5C:
embryonic day 16.5; ScribCrc/Scrib +
(more )
Specimen
5C:
(close )
|
Genetic Background:
|
involves: C57BL/6J * NMRI
|
|
Mutant Allele(s):
|
ScribCrc/Scrib+
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: brainstem nucleus |
Present |
Regionally restricted |
5C
|
Expression is in the pre-botzinger complex.
|
| TS24: medulla oblongata |
Present |
Regionally restricted |
5C
|
Expression is in the pre-botzinger complex.
|
Specimen
5D:
(close )
|
Genetic Background:
|
involves: C57BL/6J * NMRI
|
|
Mutant Allele(s):
|
ScribCrc/ScribCrc
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: brainstem nucleus |
Present |
Regionally restricted |
5D
|
Expression is in a region that was bilaterally distributed and located in the ventral part of the hindbrain corresponding to the pre-botzinger complex.
|
| TS24: medulla oblongata |
Present |
Regionally restricted |
5D
|
Expression is in a region that was bilaterally distributed and located in the ventral part of the hindbrain corresponding to the pre-botzinger complex.
|
|
Analysis Tools