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Gene Expression Data
Assay Details
Assay
Reference: J:180770 Bjerknes M, et al., Origin of the brush cell lineage in the mouse intestinal epithelium. Dev Biol. 2012 Feb 15;362(2):194-218
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:6741116
Gene symbol: Gfi1b
Gene name: growth factor independent 1B
Reporter: GFP
Direct Detection
Results
Specimen 5-1: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Double labeled: green - Gfi1b; orange - lectin UEA-I.
Structure Level Pattern Image Note
TS28: intestinal villus Present 5_1-1 , 5_1-2 Expressed in brush cells (stained positively with the lectin UEA-I).
TS28: intestine epithelium Present 5_1-1 , 5_1-2 Expressed in brush cells (stained positively with the lectin UEA-I).
Specimen 5-2: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Double labeled: green - Gfi1b; orange - Krt18 (assay MGI:6741043).
Structure Level Pattern Image Note
TS28: intestinal villus Present 5_2-2 , 5_2-1 Co-expressed with Krt18 in brush cells.
TS28: intestine epithelium Present 5_2-2 , 5_2-1 Co-expressed with Krt18 in brush cells.
Specimen 5-3: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Double labeled: green - Gfi1b; orange - Trpm5 (assay MGI:6741046).
Structure Level Pattern Image Note
TS28: intestinal villus Present 5_3-1 , 5_3-2 Co-expressed with Trpm5 in brush cells.
TS28: intestine epithelium Present 5_3-1 , 5_3-2 Co-expressed with Trpm5 in brush cells.
Specimen 5-4: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Double labeled: green - Gfi1b; orange - Ptgs1 (assay MGI:6741048).
Structure Level Pattern Image Note
TS28: intestinal villus Present 5_4-1 , 5_4-2 Co-expressed with Ptgs1 in brush cells.
TS28: intestine epithelium Present 5_4-1 , 5_4-2 Co-expressed with Ptgs1 in brush cells.
Specimen 5-5: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Double labeled: green - Gfi1b; orange - Dclk1 (assay MGI:6741049).
Structure Level Pattern Image Note
TS28: intestinal villus Present 5_5-1 , 5_5-2 Co-expressed with Dclk1 in brush cells.
TS28: intestine epithelium Present 5_5-1 , 5_5-2 Co-expressed with Dclk1 in brush cells.
Specimen 5-6: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Double labeled: green - Gfi1b; orange - Gfi1b (assay MGI:6741052).
Structure Level Pattern Image Note
TS28: intestinal villus Present 5_6-2 , 5_6-1 Expressed in brush cells.
TS28: intestine epithelium Present 5_6-2 , 5_6-1 Expressed in brush cells.
Specimen 6 left: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Double labeled: green - Gfi1b; orange - Insm1 (assay MGI:6741065).
Structure Level Pattern Image Note
TS28: intestinal villus Present 6_1 , 6_3 Expressed in brush cells (Insm1-).
TS28: intestine epithelium Present 6_1 , 6_3 Expressed in brush cells (Insm1-).
Specimen 6 right: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Double labeled: green - Gfi1b; orange - Atoh1 (assay MGI:6741099).
Structure Level Pattern Image Note
TS28: intestinal villus Present 6_4 , 6_2 Expressed in brush cells (Atoh1-).
TS28: intestine epithelium Present 6_4 , 6_2 Expressed in brush cells (Atoh1-).
Specimen 7A: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Structure Level Pattern Image Note
TS28: intestinal villus Present 7A Expressed in an immature brush cell just above the crypt base.
TS28: intestine epithelium Present 7A Expressed in an immature brush cell just above the crypt base.
Specimen 7B: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Double labeled: green - Gfi1b; orange - Atoh1 (assay MGI:6741099).
Structure Level Pattern Image Note
TS28: intestinal villus Present 7B Expressed in immature brush cells (occasionally seen in mitosis, Atoh1-) in the lower crypt.
TS28: intestine epithelium Present 7B Expressed in immature brush cells (occasionally seen in mitosis, Atoh1-) in the lower crypt.
Specimen 9-1: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Double labeled: green - Gfi1b; orange - Chga (assay MGI:6741101).
Structure Level Pattern Image Note
TS28: intestinal villus Present 9_1-1 , 9_1-2 Expressed in brush cells (Chga-).
TS28: intestine epithelium Present 9_1-1 , 9_1-2 Expressed in brush cells (Chga-).
Specimen 9-2: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Double labeled: green - Gfi1b; orange - lectin UEA-I.
Structure Level Pattern Image Note
TS28: intestinal villus Present 9_2-2 , 9_2-1 Expressed in brush cells (UEA-1+).
TS28: intestine epithelium Present 9_2-2 , 9_2-1 Expressed in brush cells (UEA-1+).
Specimen 9-3: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Double labeled: green - Gfi1b; orange - Krt18 (assay MGI:6741043).
Structure Level Pattern Image Note
TS28: intestinal villus Present 9_3-1 , 9_3-2 Co-expressed with Krt18 in brush cells.
TS28: intestine epithelium Present 9_3-1 , 9_3-2 Co-expressed with Krt18 in brush cells.
Specimen 9-4: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Double labeled: green - Gfi1b; orange - Trpm5 (assay MGI:6741046).
Structure Level Pattern Image Note
TS28: intestinal villus Present 9_4-2 , 9_4-1 Expressed in brush cells (Trpm5+).
TS28: intestine epithelium Present 9_4-2 , 9_4-1 Expressed in brush cells (Trpm5+).
Specimen 9-5: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Double labeled: green - Gfi1b; orange - Dclk1 (assay MGI:6741049).
Structure Level Pattern Image Note
TS28: intestinal villus Present 9_5-1 , 9_5-2 Co-expressed with Dclk1 in brush cells.
TS28: intestine epithelium Present 9_5-1 , 9_5-2 Co-expressed with Dclk1 in brush cells.
Specimen 9-6: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Double labeled: green - Gfi1b; orange - Gfi1b (assay MGI:6741052).
Structure Level Pattern Image Note
TS28: intestinal villus Present 9_6-1 , 9_6-2 Expressed in brush cells.
TS28: intestine epithelium Present 9_6-1 , 9_6-2 Expressed in brush cells.
Specimen 11A: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Triple labeled: green - Gfi1b; orange - Dclk1 (assay MGI:6741049); red - Mki67 (assay MGI:6741111).
Structure Level Pattern Image Note
TS28: intestinal villus Present 11A-1 , 11A-2 Expressed in the immature proliferating cells that have not begun to accumulate Dclk1.
TS28: intestine epithelium Present 11A-1 , 11A-2 Expressed in the immature proliferating cells that have not begun to accumulate Dclk1.
Specimen 11B: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Triple labeled: green - Gfi1b; orange - Dclk1 (assay MGI:6741049); red - Mki67 (assay MGI:6741111).
Structure Level Pattern Image Note
TS28: intestinal villus Present 11B-2 , 11B-1 Co-expressed with Dclk1 in brush cells (Mki67-).
TS28: intestine epithelium Present 11B-2 , 11B-1 Co-expressed with Dclk1 in brush cells (Mki67-).
Specimen 11C: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Triple labeled: green - Gfi1b; orange - Dclk1 (assay MGI:6741049); red - Mki67 (assay MGI:6741111).
Structure Level Pattern Image Note
TS28: intestinal villus Present 11C-1 , 11C-2 Co-expressed with Dclk1 in brush cells (rare examples of Mki67+ Dclk1+).
TS28: intestine epithelium Present 11C-1 , 11C-2 Co-expressed with Dclk1 in brush cells (rare examples of Mki67+ Dclk1+).
Specimen 13A: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Triple labeled: green - Gfi1b; orange - Neurog3 (assay MGI:6741112); red - Mki67 (assay MGI:6741111).
Structure Level Pattern Image Note
TS28: intestinal villus Present 13A-2 , 13A-1 Mki67+ Gfi1b+ Neurog3- brush cells were shown.
TS28: intestine epithelium Present 13A-2 , 13A-1 Mki67+ Gfi1b+ Neurog3- brush cells were shown.
Specimen 13B: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Triple labeled: green - Gfi1b; orange - Neurog3 (assay MGI:6741112); red - Mki67 (assay MGI:6741111).
Structure Level Pattern Image Note
TS28: intestinal villus Present 13B-1 , 13B-2 Mki67+ Gfi1b+ Neurog3- brush cells were shown.
TS28: intestine epithelium Present 13B-1 , 13B-2 Mki67+ Gfi1b+ Neurog3- brush cells were shown.
Specimen 13C: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Triple labeled: green - Gfi1b; red - Mki67 (assay MGI:6741111); orange - Atoh1 (assay MGI:6741099).
Structure Level Pattern Image Note
TS28: intestinal villus Present 13C-2 , 13C-1 Mki67+ Gfi1b+ Atoh1- brush cells were shown.
TS28: intestine epithelium Present 13C-2 , 13C-1 Mki67+ Gfi1b+ Atoh1- brush cells were shown.
Specimen 13D: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Triple labeled: green - Gfi1b; red - Mki67 (assay MGI:6741111); orange - Atoh1 (assay MGI:6741099).
Structure Level Pattern Image Note
TS28: intestinal villus Present 13D-1 , 13D-2 Mki67+ Gfi1b+ Atoh1- brush cells were shown.
TS28: intestine epithelium Present 13D-1 , 13D-2 Mki67+ Gfi1b+ Atoh1- brush cells were shown.
Specimen 13E: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Triple labeled: green - Gfi1b; red - Mki67 (assay MGI:6741111); orange - Atoh1 (assay MGI:6741099).
Structure Level Pattern Image Note
TS28: intestinal villus Present 13E-2 , 13E-1 Mki67- Gfi1b+ Atoh1- brush cells were shown.
TS28: intestine epithelium Present 13E-2 , 13E-1 Mki67- Gfi1b+ Atoh1- brush cells were shown.
Specimen 16B: postnatal; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Double labeled: green - Gfi1b; orange - Hes1 (assay MGI:6741115).
Structure Level Pattern Image Note
TS28: crypt of lieberkuhn Present 16B-1 , 16B-2 Expressed in an increasing gradient of differentiation (increasing gradient of cell size and content) as brush lineage cells migrated up the crypt.
TS28: intestine epithelium Present 16B-1 , 16B-2 Expressed in an increasing gradient of differentiation (increasing gradient of cell size and content) as brush lineage cells migrated up the crypt.
Specimen 17A: embryonic day 18.5; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Triple labeled: green - Gfi1b; orange - Insm1 (assay MGI:6741065); red - Dclk1 (assay MGI:67411050).
Structure Level Pattern Image Note
TS26: intestinal villus Present Regionally restricted 17A-1 , 17A-2 , 17A-3 Co-expressed with Dclk1 in brush cells in the intervillus trough in the proximal intestinal epithelium, only rarely in more distal regions.
TS26: intestine epithelium Present Regionally restricted 17A-1 , 17A-2 , 17A-3 Co-expressed with Dclk1 in brush cells in the intervillus trough in the proximal intestinal epithelium, only rarely in more distal regions.
Specimen 17B: embryonic day 18.5; Gfi1btm1Tmo/Gfi1b+ (more )
Structure Level Pattern Image Note
TS26: intestinal villus Present 17B Occasionally, expressed in relatively mature brush cells.
TS26: intestine epithelium Present 17B Occasionally, expressed in relatively mature brush cells.
Specimen 17C: embryonic day 18.5; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Triple labeled: green - Gfi1b; orange - Insm1 (assay MGI:6741065); red - Dclk1 (assay MGI:67411050).
Structure Level Pattern Image Note
TS26: intestinal villus Present 17C-2 , 17C-1 Co-expressed with Dclk1 in relatively mature brush cells.
TS26: intestine epithelium Present 17C-2 , 17C-1 Co-expressed with Dclk1 in relatively mature brush cells.
Specimen not shown: embryonic day 18.5; Gfi1btm1Tmo/Gfi1b+ (more )
Structure Level Pattern Image Note
TS26: stomach epithelium Present
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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