About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:180770 Bjerknes M, et al., Origin of the brush cell lineage in the mouse intestinal epithelium. Dev Biol. 2012 Feb 15;362(2):194-218
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6741050
Gene symbol: Dclk1
Gene name: doublecortin-like kinase 1
Antibody: Anti-DCAMKL1
Detection system: Secondary antibody coupled to Alexa Fluor 647
Results
Specimen 3A: postnatal (more )
Note: Double labeled: red - Dclk1; orange - Insm1 (assay MGI:6741065).
Structure Level Pattern Image Note
TS28: intestinal villus Present 3A-4 , 3A-2 Coexpressed with Insm1 in a subset of enteroendocrine cells.
TS28: intestine epithelium Present 3A-4 , 3A-2 Coexpressed with Insm1 in a subset of enteroendocrine cells.
Specimen 3B: postnatal (more )
Note: Double labeled: red - Dclk1; orange - Insm1 (assay MGI:6741065).
Structure Level Pattern Image Note
TS28: intestinal villus Present 3B-2 , 3B-4 Expressed in brush cells, which were Insm-.
TS28: intestine epithelium Present 3B-2 , 3B-4 Expressed in brush cells, which were Insm-.
Specimen 14 Villi-1: postnatal; Atoh1tm4.1Hzo/Atoh1+ (more )
Note: Triple labeled: red - Dclk1; orange - Insm1 (assay MGI:6741065); green - Atoh1 (assay MGI:67411117).
Structure Level Pattern Image Note
TS28: intestinal villus Present 14_1-3 , 14_1-1 Expressed in brush cells (Atoh1- Insm1-).
TS28: intestine epithelium Present 14_1-3 , 14_1-1 Expressed in brush cells (Atoh1- Insm1-).
Specimen 14 Villi-2: postnatal; Atoh1tm4.1Hzo/Atoh1+ (more )
Note: Triple labeled: red - Dclk1; orange - Insm1 (assay MGI:6741065); green - Atoh1 (assay MGI:67411117).
Structure Level Pattern Image Note
TS28: intestinal villus Present 14_2-1 , 14_2-3 Expressed in brush cells (Atoh1- Insm1-).
TS28: intestine epithelium Present 14_2-1 , 14_2-3 Expressed in brush cells (Atoh1- Insm1-).
Specimen 14 Crypts-1: postnatal; Atoh1tm4.1Hzo/Atoh1+ (more )
Note: Triple labeled: red - Dclk1; orange - Insm1 (assay MGI:6741065); green - Atoh1 (assay MGI:67411117).
Structure Level Pattern Image Note
TS28: crypt of lieberkuhn Weak 14_3-1 , 14_3-3 Expressed in immature brush cells (Atoh1+ Insm1-) in the lower crypt.
TS28: intestine epithelium Weak 14_3-1 , 14_3-3 Expressed in immature brush cells (Atoh1+ Insm1-) in the lower crypt.
Specimen 14 Crypts-2: postnatal; Atoh1tm4.1Hzo/Atoh1+ (more )
Note: Triple labeled: red - Dclk1; orange - Insm1 (assay MGI:6741065); green - Atoh1 (assay MGI:67411117).
Structure Level Pattern Image Note
TS28: crypt of lieberkuhn Present 14_4-3 , 14_4-1 Expressed in mature brush cells (Atoh1- Insm1-). Weakly expressed in immature brush cells (Atoh1+ Insm1-) in the lower crypt.
TS28: intestine epithelium Present 14_4-3 , 14_4-1 Expressed in mature brush cells (Atoh1- Insm1-). Weakly expressed in immature brush cells (Atoh1+ Insm1-) in the lower crypt.
Specimen 17A: embryonic day 18.5; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Triple labeled: red - Dclk1; green - Gfi1b (assay MGI:6741116); orange - Insm1 (assay MGI:67411065).
Structure Level Pattern Image Note
TS26: intestinal villus Present Regionally restricted 17A-1 , 17A-4 Co-expressed with Gfi1b in brush cells in the intervillus trough.
TS26: intestine epithelium Present Regionally restricted 17A-1 , 17A-4 Co-expressed with Gfi1b in brush cells in the intervillus trough.
Specimen 17C: embryonic day 18.5; Gfi1btm1Tmo/Gfi1b+ (more )
Note: Triple labeled: red - Dclk1; green - Gfi1b (assay MGI:6741116); orange - Insm1 (assay MGI:67411065).
Structure Level Pattern Image Note
TS26: intestinal villus Present 17C-3 , 17C-1 Co-expressed with Gfi1b in relatively mature brush cells.
TS26: intestine epithelium Present 17C-3 , 17C-1 Co-expressed with Gfi1b in relatively mature brush cells.
Specimen 18 villi +/-: embryonic day 18.5; Atoh1tm1Hzo/Atoh1+ (more )
Note: Double labeled: red - Dclk1; orange - Insm1 (assay MGI:6741065).
Structure Level Pattern Image Note
TS26: intestinal villus Present Regionally restricted 18_upper left Expressed in brush cells in the intervillus trough of the most proximal intestinal epithelium.
TS26: intestine epithelium Present Regionally restricted 18_upper left Expressed in brush cells in the intervillus trough of the most proximal intestinal epithelium.
Specimen 18 villi -/-: embryonic day 18.5; Atoh1tm1Hzo/Atoh1tm1Hzo (more )
Note: Double labeled: red - Dclk1; orange - Insm1 (assay MGI:6741065).
Structure Level Pattern Image Note
TS26: intestinal villus Absent 18_upper right
TS26: intestine epithelium Absent 18_upper right
Specimen 18 intervillus +/-: embryonic day 18.5; Atoh1tm1Hzo/Atoh1+ (more )
Note: Double labeled: red - Dclk1; orange - Insm1 (assay MGI:6741065).
Structure Level Pattern Image Note
TS26: intestinal villus Present Regionally restricted 18_lower left Expressed in brush cells in the intervillus trough of the most proximal intestinal epithelium.
TS26: intestine epithelium Present Regionally restricted 18_lower left Expressed in brush cells in the intervillus trough of the most proximal intestinal epithelium.
Specimen 18 intervillus -/-: embryonic day 18.5; Atoh1tm1Hzo/Atoh1tm1Hzo (more )
Note: Double labeled: red - Dclk1; orange - Insm1 (assay MGI:6741065).
Structure Level Pattern Image Note
TS26: intestinal villus Absent 18_lower right
TS26: intestine epithelium Absent 18_lower right
Specimen not shown: embryonic day 18.5; Atoh1tm1Hzo/Atoh1+ (more )
Structure Level Pattern Image Note
TS26: stomach epithelium Present Expressed in brush cells (Insm1-).
Specimen not shown: embryonic day 18.5; Atoh1tm1Hzo/Atoh1tm1Hzo (more )
Structure Level Pattern Image Note
TS26: stomach epithelium Present Expressed in brush cells (Insm1-).
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
05/07/2024
MGI 6.23 		The Jackson Laboratory