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Gene Expression Data
Assay Details
Assay
Reference: J:278517 Li M, et al., The histone modification reader ZCWPW1 is required for meiosis prophase I in male but not in female mice. Sci Adv. 2019;5(8):eaax1101
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6727342
Gene symbol: Dmc1
Gene name: DNA meiotic recombinase 1
Antibody: anti-Dmc1 (H-100; sc-22768)
Detection system: Alexa Fluor 488
Results
Specimen 4O: postnatal day 25 (more )
Note: Leptotene stage. Double labeled: green - Dmc1; red - Sycp3 (assay MGI:6727331).
Structure Level Pattern Image Note
TS28: spermatocyte Present 4O
Specimen 4P: postnatal day 25 (more )
Note: Zygotene stage. Double labeled: green - Dmc1; red - Sycp3 (assay MGI:6727331).
Structure Level Pattern Image Note
TS28: spermatocyte Present 4P
Specimen 4Q: postnatal day 25 (more )
Note: Pachytene stage. Double labeled: green - Dmc1; red - Sycp3 (assay MGI:6727331).
Structure Level Pattern Image Note
TS28: spermatocyte Present 4Q
Specimen 4R: postnatal day 25; Zcwpw1em1Kliu/Zcwpw1em1Kliu (more )
Note: Leptotene stage. Double labeled: green - Dmc1; red - Sycp3 (assay MGI:6727331).
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 4R Number of foci of experssion comparable to that of +/+ spermatocytes at leptotene stage.
Specimen 4S: postnatal day 25; Zcwpw1em1Kliu/Zcwpw1em1Kliu (more )
Note: Zygotene stage. Double labeled: green - Dmc1; red - Sycp3 (assay MGI:6727331).
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 4S Number of foci of expression comparable to that of +/+ spermatocytes at early zygotene stage.
Specimen Late zygotene not shown: postnatal day 25; Zcwpw1em1Kliu/Zcwpw1em1Kliu (more )
Structure Level Pattern Image Note
TS28: spermatocyte Present Significantly higher number of foci of expression in spermatocytes at late zygotene stage compared to +/+ spermatocytes.
Specimen 4T: postnatal day 25; Zcwpw1em1Kliu/Zcwpw1em1Kliu (more )
Note: Pachytene-like. Double labeled: green - Dmc1; red - Sycp3 (assay MGI:6727331).
Structure Level Pattern Image Note
TS28: spermatocyte Present Regionally restricted 4T Significantly higher number of foci of expression in spermatocytes at pachytene-like stages compared to +/+ spermatocytes.
Specimen 5R: embryonic day 17.5 (more )
Note: Zygotene stage. Double labeled: green - Dmc1; red - Sycp3 (assay MGI:6727331).
Structure Level Pattern Image Note
TS25: oocyte Present 5R
Specimen 5S: embryonic day 17.5; Zcwpw1em1Kliu/Zcwpw1em1Kliu (more )
Note: Zygotene stage. Double labeled: green - Dmc1; red - Sycp3 (assay MGI:6727331).
Structure Level Pattern Image Note
TS25: oocyte Present Regionally restricted 5S Numbers of foci of expression comparable to +/+ oocytes at zygotene stage.
Specimen 5T: embryonic day 17.5 (more )
Note: Pachytene stage. Double labeled: green - Dmc1; red - Sycp3 (assay MGI:6727331).
Structure Level Pattern Image Note
TS25: oocyte Present 5T
Specimen 5U: embryonic day 17.5; Zcwpw1em1Kliu/Zcwpw1em1Kliu (more )
Note: Pachytene stage. Double labeled: green - Dmc1; red - Sycp3 (assay MGI:6727331).
Structure Level Pattern Image Note
TS25: oocyte Present Regionally restricted 5U Numbers of foci of expression comparable to +/+ oocytes at pachytene stage.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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10/22/2024
MGI 6.24 		The Jackson Laboratory