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Gene Expression Data
Assay Details
Assay
Reference: J:278517 Li M, et al., The histone modification reader ZCWPW1 is required for meiosis prophase I in male but not in female mice. Sci Adv. 2019;5(8):eaax1101
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6727339
Gene symbol: Rpa2
Gene name: replication protein A2
Antibody: anti-RPA32/RPA2 [EPR2877Y] (ab76420)
Detection system: Alexa Fluor 488
Results
Specimen 4A: postnatal day 25 (more )
Note: Leptotene stage. Double labeled: green - Rpa2; red - Sycp3 (assay MGI:6727331).
Structure Level Pattern Image Note
TS28: spermatocyte Present 4A
Specimen 4B: postnatal day 25 (more )
Note: Zygotene stage. Double labeled: green - Rpa2; red - Sycp3 (assay MGI:6727331).
Structure Level Pattern Image Note
TS28: spermatocyte Present 4B
Specimen 4C: postnatal day 25 (more )
Note: Pachytene stage. Double labeled: green - Rpa2; red - Sycp3 (assay MGI:6727331).
Structure Level Pattern Image Note
TS28: spermatocyte Present 4C
Specimen 4D: postnatal day 25; Zcwpw1em1Kliu/Zcwpw1em1Kliu (more )
Note: Leptotene stage. Double labeled: green - Rpa2; red - Sycp3 (assay MGI:6727331).
Structure Level Pattern Image Note
TS28: spermatocyte Present 4D
Specimen 4E: postnatal day 25; Zcwpw1em1Kliu/Zcwpw1em1Kliu (more )
Note: Zygotene stage. Double labeled: green - Rpa2; red - Sycp3 (assay MGI:6727331).
Structure Level Pattern Image Note
TS28: spermatocyte Present 4E
Specimen 4F: postnatal day 25; Zcwpw1em1Kliu/Zcwpw1em1Kliu (more )
Note: Pachytene-like. Double labeled: green - Rpa2; red - Sycp3 (assay MGI:6727331).
Structure Level Pattern Image Note
TS28: spermatocyte Present 4F Number of foci of expression persisted in pachytene/pachytene-like spermatocytes.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory