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Gene Expression Data
Assay Details
Assay
Reference: J:290862 Fulmer D, et al., Desert hedgehog-primary cilia cross talk shapes mitral valve tissue by organizing smooth muscle actin. Dev Biol. 2020 Jul 1;463(1):26-38
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6718503
Gene symbol: Acta2
Gene name: actin alpha 2, smooth muscle, aorta
Antibody: Anti-smooth muscle alpha-Actin (clone 1A4)
Detection system: Secondary antibody
Results
Specimen 5A E10.5: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: atrium cardiac muscle Strong 5A upper left
TS17: primitive ventricle cardiac muscle Strong 5A upper left
TS17: atrioventricular canal cardiac muscle Absent 5A upper right , 5A upper left
Specimen 5A E13.5: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: atrium cardiac muscle Strong 5A middle left
TS21: ventricle cardiac muscle Strong 5A middle left
TS21: atrioventricular canal cardiac muscle Present 5A middle right , 5A middle left
TS21: mitral valve Present Regionally restricted 5A middle right , 5A middle left Expressed in a core of cells localized to the fusion region of the superior and inferior cushions as well as a few interstitial cells within the tip of the anterior and posterior leaflets.
Specimen 5A E15.5: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: mitral valve Present Regionally restricted 5A lower right , 5A lower left Expressed in a core of cells localized to the fusion region of the superior and inferior cushions as well as a few interstitial cells within the tip of the anterior and posterior leaflets.
TS23: mitral valve leaflet Present Regionally restricted 5A lower right , 5A lower left Expression became more pronounced within interstitial cells localized to the distal tips.
Specimen 5B: postnatal day 0 (more )
Structure Level Pattern Image Note
TS27: mitral valve Present 5B Expression continued to increase within interstitial cells localized to the distal tips.
Specimen 5C control 3D: postnatal day 0 (more )
Structure Level Pattern Image Note
TS27: mitral valve Strong Regionally restricted 5C upper left Expressed within interstitial cells localized to the distal tips. Expressed primarily in regions of apposition between the anterior and posterior mitral leaflets.
Specimen 5C control: postnatal day 0 (more )
Structure Level Pattern Image Note
TS27: mitral valve Strong Regionally restricted 5C lower left Expressed within interstitial cells localized to the distal tips. Expressed primarily in regions of apposition between the anterior and posterior mitral leaflets.
Specimen 5C Dhh cKO 3D: postnatal day 0; Dhhtm1Ics/Dhhtm1Ics, Nfatc1tm1.1(cre)Bz/Nfatc1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS27: mitral valve Present 5C upper right Reduced expression compared with the control.
Specimen 5C Dhh cKO 2: postnatal day 0; Dhhtm1Ics/Dhhtm1Ics, Nfatc1tm1.1(cre)Bz/Nfatc1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS27: mitral valve Present 5C lower right Reduced expression compared with the control.
Specimen 5D control: postnatal day 0; Ift88tm1Bky/Ift88+ (more )
Structure Level Pattern Image Note
TS27: mitral valve Present 5D left upper , 5D left lower
Specimen 5D Ift88 cKO: postnatal day 0; Ift88tm1Bky/Ift88tm1Bky, Nfatc1tm1.1(cre)Bz/Nfatc1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS27: mitral valve Present 5D right lower , 5D right upper Reduced expression compared with the control.
Specimen S12 WT: postnatal day 0 (more )
Note: Double labeled: red - Acta2; green - Vcan (assay MGI:6718608).
Structure Level Pattern Image Note
TS27: mitral valve Present S12_upper middle , S12_upper right Expression did not overlap with Vcan.
Specimen S12 Het: postnatal day 0; Dhhtm1Ics/Dhh+, Nfatc1tm1.1(cre)Bz/Nfatc1+ (more )
Note: Conditional mutant. Double labeled: red - Acta2; green - Vcan (assay MGI:6718608).
Structure Level Pattern Image Note
TS27: mitral valve Present S12_middle right , S12_middle middle Distribution and organization were affected compared to controls.
Specimen S12 cKO: postnatal day 0; Dhhtm1Ics/Dhhtm1Ics, Nfatc1tm1.1(cre)Bz/Nfatc1+ (more )
Note: Conditional mutant. Double labeled: red - Acta2; green - Vcan (assay MGI:6718608).
Structure Level Pattern Image Note
TS27: mitral valve Present S12_lower right , S12_lower middle Distribution and organization were affected compared to controls.
Specimen S13 WT: postnatal day 0 (more )
Note: Double labeled: red - Acta2; green - Col1a1 (assay MGI:6718609).
Structure Level Pattern Image Note
TS27: mitral valve Present S13_upper middle , S13_upper right Expression did not completely overlap with Col1a1. Although some overlap was detected, Acta2 appeared to largely be excluded from Col1a1 expressing cells.
Specimen S13 Het: postnatal day 0; Dhhtm1Ics/Dhh+, Nfatc1tm1.1(cre)Bz/Nfatc1+ (more )
Note: Conditional mutant. Double labeled: red - Acta2; green - Col1a1 (assay MGI:6718609).
Structure Level Pattern Image Note
TS27: mitral valve Present S13_middle middle , S13_middle right Expressed in a reciprocal pattern with Col1a1 compared to controls.
Specimen S13 cKO: postnatal day 0; Dhhtm1Ics/Dhhtm1Ics, Nfatc1tm1.1(cre)Bz/Nfatc1+ (more )
Note: Conditional mutant. Double labeled: red - Acta2; green - Col1a1 (assay MGI:6718609).
Structure Level Pattern Image Note
TS27: mitral valve Present S13_lower right , S13_lower middle Expressed in a reciprocal pattern with Col1a1 compared to controls.
Specimen S14: embryonic day 15.5; Gt(ROSA)26Sortm1(EYFP)Cos/Gt(ROSA)26Sor+, Tg(Wt1-cre)#Jbeb/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS23: mitral valve Present S14_SMA , S14_merge Expressed in interstitial cells, which were not derived from the epicardial lineage.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory