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Gene Expression Data
Assay Details
Assay
Reference: J:231231 Abby E, et al., Implementation of meiosis prophase I programme requires a conserved retinoid-independent stabilizer of meiotic transcripts. Nat Commun. 2016;7:10324
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6716297
Gene symbol: Rpa2
Gene name: replication protein A2
Antibody: anti-RPA32/RPA2 (4E4, #2208)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen S6a Lepto Meioc+/+: postnatal (more )
Note: Leptotene. Double labeled: green - Rpa2; red - Sycp3 (assay MGI:6695313).
Structure Level Pattern Image Note
TS28: spermatocyte Present S6a Lepto Meioc+/+ Rpa2 , S6a Lepto Meioc+/+ Rpa2/Sycp3
Specimen S6a Lepto Meioc-/-: postnatal; Meioctm1a(KOMP)Wtsi/Meioctm1a(KOMP)Wtsi (more )
Note: Leptotene. Double labeled: green - Rpa2; red - Sycp3 (assay MGI:6695313).
Structure Level Pattern Image Note
TS28: spermatocyte Present S6a Lepto Meioc-/- Rpa2 , S6a Lepto Meioc-/- Rpa2/Sycp3 Rpa2-positive foci formed normally but persisted in mutants.
Specimen S6a Zygo Meioc+/+: postnatal (more )
Note: Zygotene. Double labeled: green - Rpa2; red - Sycp3 (assay MGI:6695313).
Structure Level Pattern Image Note
TS28: spermatocyte Present S6a Zygo Meioc+/+ Rpa2/Sycp3 , S6a Zygo Meioc+/+ Rpa2
Specimen S6a Zygo-like Meioc-/- top: postnatal; Meioctm1a(KOMP)Wtsi/Meioctm1a(KOMP)Wtsi (more )
Note: Zygotene-like. Double labeled: green - Rpa2; red - Sycp3 (assay MGI:6695313).
Structure Level Pattern Image Note
TS28: spermatocyte Present S6a Zygo-Like Meioc-/- Rpa2/Sycp3 top , S6a Zygo-Like Meioc-/- Rpa2 top Rpa2-positive foci formed normally but persisted in mutants.
Specimen S6a Pachy Meioc+/+: postnatal (more )
Note: Pachytene. Double labeled: green - Rpa2; red - Sycp3 (assay MGI:6695313).
Structure Level Pattern Image Note
TS28: spermatocyte Present S6a Pachy Meioc+/+ Rpa2/Sycp3 , S6a Pachy Meioc+/+ Rpa2
Specimen S6a Zygo-like Meioc-/- bottom: postnatal; Meioctm1a(KOMP)Wtsi/Meioctm1a(KOMP)Wtsi (more )
Note: Zygotene-like. Double labeled: green - Rpa2; red - Sycp3 (assay MGI:6695313).
Structure Level Pattern Image Note
TS28: spermatocyte Present S6a Zygo-Like Meioc-/- Rpa2 bottom , S6a Zygo-Like Meioc-/- Rpa2/Sycp3 bottom Rpa2-positive foci formed normally but persisted in mutants.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
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